PMID- 29992645
OWN - NLM
STAT- MEDLINE
DCOM- 20191114
LR  - 20231213
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Linking)
VI  - 48
IP  - 12
DP  - 2018 Dec
TI  - Gly m 5/Gly m 8 fusion component as a potential novel candidate molecule for 
      diagnosing soya bean allergy in Japanese children.
PG  - 1726-1734
LID - 10.1111/cea.13231 [doi]
AB  - BACKGROUND: Soya bean is a major food allergen in children. Component-resolved 
      diagnostics has improved the accuracy of diagnosing immunoglobulin E 
      (IgE)-mediated food allergies. OBJECTIVE: We aimed to develop a novel component 
      for the diagnosis of soya bean allergy using recombinant technology. METHODS: 
      Japanese paediatric patients with suspected soya bean allergy (n = 91) were 
      included, and symptomatic (n = 40) and asymptomatic (n = 51) cases were divided 
      through oral food challenge testing. Specific IgE (sIgE) antibodies to each 
      recombinant allergen component were analysed by enzyme-linked immunosorbent 
      assay, and the diagnostic performances of the components were assessed by area 
      under the receiver operating characteristic curves (AUC). RESULTS: Among the 
      recombinant components, sIgE antibody levels to Gly m 8 showed the highest AUC 
      (0.706). A combination of Gly m 8 and alpha' subunit of Gly m 5, improved the 
      diagnostic performance of the single components. Moreover, the N-terminal 
      extension region of alpha' subunit of Gly m 5, which has low cross-reactivity among 
      the vicilins, showed higher diagnostic performance (AUC 0.695) than the 
      full-length alpha' subunit of Gly m 5 (AUC 0.613). Based on these findings, we 
      developed a fusion protein of Gly m 8 plus the extension region of alpha' subunit of 
      Gly m 5; this fusion protein was very efficient for diagnosing soya bean allergy 
      (AUC 0.801). CONCLUSION: A fusion protein of Gly m 8 and the extension region of 
      alpha' subunit of Gly m 5 could potentially diagnose soya bean allergy in paediatric 
      patients. Fusion proteins may be useful for producing novel allergen components 
      with improved diagnostic value.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Maruyama, Nobuyuki
AU  - Maruyama N
AUID- ORCID: 0000-0002-1369-9629
AD  - Laboratory of Food Quality Design and Development, Graduate School of 
      Agriculture, Kyoto University, Kyoto, Japan.
FAU - Sato, Sakura
AU  - Sato S
AD  - Clinical Research Center for Allergy and Rheumatology, Sagamihara National 
      Hospital, Kanagawa, Japan.
FAU - Cabanos, Cerrone
AU  - Cabanos C
AD  - Laboratory of Food Quality Design and Development, Graduate School of 
      Agriculture, Kyoto University, Kyoto, Japan.
FAU - Tanaka, Akira
AU  - Tanaka A
AD  - Thermo Fisher Diagnostics, Tokyo, Japan.
FAU - Ito, Komei
AU  - Ito K
AD  - Department of Allergy, Aichi Children's Health and Medical Center, Obu, Japan.
FAU - Ebisawa, Motohiro
AU  - Ebisawa M
AD  - Clinical Research Center for Allergy and Rheumatology, Sagamihara National 
      Hospital, Kanagawa, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180813
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Globulins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Seed Storage Proteins)
RN  - 0 (Soybean Proteins)
RN  - 0 (beta-conglycinin protein, Glycine max)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Antigens, Plant/*immunology
MH  - Child
MH  - Cross Reactions/immunology
MH  - Female
MH  - Food Hypersensitivity/*diagnosis/epidemiology/*immunology
MH  - Globulins/*immunology
MH  - Humans
MH  - Immunoassay
MH  - Immunoglobulin E/blood/immunology
MH  - Japan/epidemiology
MH  - Male
MH  - Public Health Surveillance
MH  - Recombinant Proteins/immunology
MH  - Reproducibility of Results
MH  - Seed Storage Proteins/*immunology
MH  - Sensitivity and Specificity
MH  - Soybean Proteins/*immunology
MH  - Glycine max/*adverse effects/genetics/immunology
OTO - NOTNLM
OT  - component-resolved diagnostics
OT  - epitope
OT  - molecular allergology
OT  - soya bean
EDAT- 2018/07/12 06:00
MHDA- 2019/11/15 06:00
CRDT- 2018/07/12 06:00
PHST- 2017/10/06 00:00 [received]
PHST- 2018/05/14 00:00 [accepted]
PHST- 2018/07/12 06:00 [pubmed]
PHST- 2019/11/15 06:00 [medline]
PHST- 2018/07/12 06:00 [entrez]
AID - 10.1111/cea.13231 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2018 Dec;48(12):1726-1734. doi: 10.1111/cea.13231. Epub 2018 
      Aug 13.