PMID- 29993359
OWN - NLM
STAT- MEDLINE
DCOM- 20190528
LR  - 20210702
IS  - 1465-2080 (Electronic)
IS  - 1350-0872 (Linking)
VI  - 164
IP  - 9
DP  - 2018 Sep
TI  - Cinnamaldehyde disrupts biofilm formation and swarming motility of Pseudomonas 
      aeruginosa.
PG  - 1087-1097
LID - 10.1099/mic.0.000692 [doi]
AB  - Bacterial biofilms can cause serious health care complications associated with 
      increased morbidity and mortality. There is an urge to discover and develop new 
      biofilm inhibitors from natural products or by modifying natural compounds or 
      understanding the modes of action of existing compounds. Cinnamaldehyde (CAD), 
      one of the major components of cinnamon oil, has been demonstrated to act as an 
      antimicrobial agent against a number of Gram-negative and Gram-positive 
      pathogens, including Pseudomonas aeruginosa, Helicobacter pylori and Listeria 
      monocytogenes. Despite the mechanism of action of CAD against the model organism 
      P. aeruginosa being undefined, based on its antimicrobial properties, we 
      hypothesized that it may disrupt preformed biofilms of P. aeruginosa. The minimum 
      inhibitory concentration (MIC) of CAD for planktonic P. aeruginosa was determined 
      to be 11.8 mM. Membrane depolarization assays demonstrated disruption of the 
      transmembrane potential of P. aeruginosa. CAD at 5.9 mM (0.5 MIC) disrupted 
      preformed biofilms by 75.6 % and 3 mM CAD (0.25 MIC) reduced the intracellular 
      concentrations of the secondary messenger, bis-(3'-5')-cyclic dimeric guanosine 
      monophosphate (c-di-GMP), which controls P. aeruginosa biofilm formation. The 
      swarming motility of P. aeruginosa was also reduced by CAD in a 
      concentration-dependent manner. Collectively, these findings show that sub-MICs 
      of CAD can disrupt biofilms and other surface colonization phenotypes through the 
      modulation of intracellular signalling processes.
FAU - Topa, Sanjida Halim
AU  - Topa SH
AD  - 1​Department of Chemistry and Biotechnology, School of Science, Swinburne 
      University of Technology, Hawthorn, Victoria, Australia.
FAU - Subramoni, Sujatha
AU  - Subramoni S
AD  - 2​Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang 
      Technological University, Nanyang Avenue, Singapore.
FAU - Palombo, Enzo A
AU  - Palombo EA
AD  - 1​Department of Chemistry and Biotechnology, School of Science, Swinburne 
      University of Technology, Hawthorn, Victoria, Australia.
FAU - Kingshott, Peter
AU  - Kingshott P
AD  - 1​Department of Chemistry and Biotechnology, School of Science, Swinburne 
      University of Technology, Hawthorn, Victoria, Australia.
FAU - Rice, Scott A
AU  - Rice SA
AD  - 2​Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang 
      Technological University, Nanyang Avenue, Singapore.
AD  - 3​The School of Biological Sciences, Nanyang Technological University, Nanyang 
      Avenue, Singapore.
AD  - 4​The ithree Institute, The University of Technology Sydney, Sydney, Australia.
FAU - Blackall, Linda L
AU  - Blackall LL
AD  - 1​Department of Chemistry and Biotechnology, School of Science, Swinburne 
      University of Technology, Hawthorn, Victoria, Australia.
AD  - 5​School of BioSciences, The University of Melbourne, Parkville, Victoria, 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180712
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Anti-Bacterial Agents)
RN  - 7864XYD3JJ (Acrolein)
RN  - SR60A3XG0F (cinnamaldehyde)
SB  - IM
MH  - Acrolein/*analogs & derivatives/pharmacology
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Biofilms/*drug effects/growth & development
MH  - Locomotion/*drug effects
MH  - Membrane Potentials/drug effects
MH  - Microbial Sensitivity Tests
MH  - Pseudomonas aeruginosa/*drug effects/physiology
MH  - Secondary Metabolism/drug effects
OTO - NOTNLM
OT  - Cinnamaldehyde
OT  - Pseudomonas aeruginosa
OT  - biofilm inhibition
OT  - c-di-GMP
OT  - natural products
EDAT- 2018/07/12 06:00
MHDA- 2019/05/29 06:00
CRDT- 2018/07/12 06:00
PHST- 2018/07/12 06:00 [pubmed]
PHST- 2019/05/29 06:00 [medline]
PHST- 2018/07/12 06:00 [entrez]
AID - 10.1099/mic.0.000692 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2018 Sep;164(9):1087-1097. doi: 10.1099/mic.0.000692. 
      Epub 2018 Jul 12.