PMID- 29996008
OWN - NLM
STAT- MEDLINE
DCOM- 20181119
LR  - 20181119
IS  - 1096-9888 (Electronic)
IS  - 1076-5174 (Linking)
VI  - 53
IP  - 10
DP  - 2018 Oct
TI  - Development of an ASE-GC-MS/MS method for detecting dinitolmide and its 
      metabolite 3-ANOT in eggs.
PG  - 976-985
LID - 10.1002/jms.4267 [doi]
AB  - An accelerated solvent extraction coupled with gas chromatography-tandem mass 
      spectrometry (ASE-GC-MS/MS) method for detecting dinitolmide residue and its 
      metabolite (3-amino-2-methyl-5-nitrobenzamide, 3-ANOT) in eggs was developed and 
      optimized. The samples were extracted using ASE with acetonitrile as the 
      extractant and were purified by passage through a neutral alumina solid-phase 
      extraction column. Then, the samples were analyzed using the GC-MS/MS method. The 
      optimized method parameters were validated according to the requirements set 
      forth by the European Union and the Food and Drug Administration. The average 
      recoveries of dinitolmide and 3-ANOT from eggs (egg white, egg yolk, and whole 
      egg) at the limit of quantification (LOQ), 0.5 maximum residue limit (MRL), 1 
      MRL, and 2 MRL were 82.74% to 87.49%, the relative standard deviations (RSDs) 
      were less than 4.63%, and the intra-day RSDs and the inter-day RSDs were 2.96% to 
      5.21% and 3.94% to 6.34%, respectively. The limits of detection and the LOQ were 
      0.8 to 2.8 mug/kg and 3.0 to 10.0 mug/kg, respectively. The decision limits (CC(alpha) 
      ) were 3001.69 to 3006.48 mug/kg, and the detection capabilities (CC(beta) ) were 
      3001.74 to 3005.22 mug/kg. Finally, the new method was successfully applied to the 
      quantitative determination of dinitolmide and 3-ANOT in 50 commercial eggs from 
      local supermarkets.
CI  - (c) 2018 John Wiley & Sons, Ltd.
FAU - Zhao, Xia
AU  - Zhao X
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Wang, Bo
AU  - Wang B
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Xie, Kaizhou
AU  - Xie K
AUID- ORCID: 0000-0002-2788-4323
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Liu, Yanan
AU  - Liu Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Zhang, Yangyang
AU  - Zhang Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Wang, Yajuan
AU  - Wang Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Liu, Chujun
AU  - Liu C
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Guo, Yawen
AU  - Guo Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Bu, Xiaona
AU  - Bu X
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Zhang, Genxi
AU  - Zhang G
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
FAU - Dai, Guojun
AU  - Dai G
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, 
      China.
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design 
      of Jiangsu Province, Yangzhou, 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, 225009, China.
LA  - eng
GR  - CARS-41-G23/China Agriculture Research System/
GR  - Yangzhou University high-end talent support program/
GR  - PAPD/Priority Academic Program Development of Jiangsu Higher Education 
      Institutions/
GR  - CX(18)3022/Project of Jiangsu Agricultural Science and Technology Independent 
      Innovation Fund/
PT  - Journal Article
DEP - 20180815
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
RN  - AOX68RY4TV (Dinitolmide)
SB  - IM
MH  - Dinitolmide/*analysis/chemistry/metabolism
MH  - Eggs/*analysis
MH  - Food Contamination/*analysis
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Limit of Detection
MH  - Linear Models
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - 3-ANOT
OT  - ASE
OT  - GC-MS/MS
OT  - dinitolmide
OT  - egg
EDAT- 2018/07/12 06:00
MHDA- 2018/11/20 06:00
CRDT- 2018/07/12 06:00
PHST- 2018/05/03 00:00 [received]
PHST- 2018/06/30 00:00 [revised]
PHST- 2018/07/02 00:00 [accepted]
PHST- 2018/07/12 06:00 [pubmed]
PHST- 2018/11/20 06:00 [medline]
PHST- 2018/07/12 06:00 [entrez]
AID - 10.1002/jms.4267 [doi]
PST - ppublish
SO  - J Mass Spectrom. 2018 Oct;53(10):976-985. doi: 10.1002/jms.4267. Epub 2018 Aug 
      15.