PMID- 29997237
OWN - NLM
STAT- MEDLINE
DCOM- 20190826
LR  - 20190826
IS  - 1470-8736 (Electronic)
IS  - 0143-5221 (Linking)
VI  - 132
IP  - 14
DP  - 2018 Jul 31
TI  - Differential long noncoding RNA expressions in peripheral blood mononuclear cells 
      for detection of acute ischemic stroke.
PG  - 1597-1614
LID - 10.1042/CS20180411 [doi]
AB  - Long noncoding RNAs (lncRNAs) have been highlighted to be involved in the 
      pathological process of ischemic stroke (IS). The purpose of the present study 
      was to investigate the expression profile of lncRNAs in peripheral blood 
      mononuclear cells (PBMCs) of acute IS patients and to explore their utility as 
      biomarkers of IS. Distinctive expression patterns of PBMC lncRNAs were identified 
      by an lncRNA microarray and individual quantitative real-time PCR (qRT-PCR) in 
      four independent sets for 206 IS, 179 healthy controls (HCs), and 55 patients 
      with transient ischemic attack (TIA). A biomarker panel (lncRNA-based combination 
      index) was established using logistic regression. LncRNA microarray analysis 
      showed 70 up-regulated and 128 down-regulated lncRNAs in IS patients. Individual 
      qRT-PCR validation demonstrated that three lncRNAs (linc-DHFRL1-4, SNHG15, and 
      linc-FAM98A-3) were significantly up-regulated in IS patients compared with HCs 
      and TIA patients. Longitudinal analysis of lncRNA expression up to 90 days after 
      IS showed that linc-FAM98A-3 normalized to control levels by day 7, while SNHG15 
      remained increased, indicating the ability of lncRNAs to monitor IS dynamics. 
      Receiver-operating characteristic (ROC) curve analysis showed that the 
      lncRNA-based combination index outperformed serum brain-derived neurotrophic 
      factor (BDNF) and neurone-specific enolase (NSE) in distinguishing IS patients 
      from TIA patients and HCs with areas under ROC curve of more than 0.84. 
      Furthermore, the combination index increased significantly after treatment and 
      was correlated with neurological deficit severity of IS. The panel of these 
      altered lncRNAs was associated with acute IS and could serve as a novel 
      diagnostic method.
CI  - (c) 2018 The Author(s). Published by Portland Press Limited on behalf of the 
      Biochemical Society.
FAU - Deng, Qi-Wen
AU  - Deng QW
AD  - Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, 
      Southeast University, Nanjing 210009, China.
AD  - Department of Neurology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing 210006, China.
FAU - Li, Shuo
AU  - Li S
AD  - Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, 
      Southeast University, Nanjing 210009, China.
FAU - Wang, Huan
AU  - Wang H
AD  - Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, 
      Southeast University, Nanjing 210009, China.
FAU - Sun, Hui-Ling
AU  - Sun HL
AD  - General Clinical Research Center, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing 210006, China.
FAU - Zuo, Lei
AU  - Zuo L
AD  - Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, 
      Southeast University, Nanjing 210009, China.
FAU - Gu, Zheng-Tian
AU  - Gu ZT
AD  - Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, 
      Southeast University, Nanjing 210009, China.
FAU - Lu, Guo
AU  - Lu G
AD  - Department of Neurology, Dezhou People's Hospital, Dezhou 253014, China.
FAU - Sun, Cai-Zhi
AU  - Sun CZ
AD  - Department of Emergency, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing 210006, China.
FAU - Zhang, Han-Qing
AU  - Zhang HQ
AD  - Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, 
      Southeast University, Nanjing 210009, China.
FAU - Yan, Fu-Ling
AU  - Yan FL
AD  - Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, 
      Southeast University, Nanjing 210009, China yanfuling218@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180731
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (RNA, Long Noncoding)
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain Ischemia/complications/diagnosis/*genetics
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Female
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology/*metabolism
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - RNA, Long Noncoding/blood/*genetics
MH  - Stroke/diagnosis/etiology/*genetics
OTO - NOTNLM
OT  - PBMC
OT  - diagnosis
OT  - ischemic stroke
OT  - lncRNA
OT  - microarray
OT  - transient ischemic attack
EDAT- 2018/07/13 06:00
MHDA- 2019/08/27 06:00
CRDT- 2018/07/13 06:00
PHST- 2018/05/15 00:00 [received]
PHST- 2018/07/02 00:00 [revised]
PHST- 2018/07/09 00:00 [accepted]
PHST- 2018/07/13 06:00 [pubmed]
PHST- 2019/08/27 06:00 [medline]
PHST- 2018/07/13 06:00 [entrez]
AID - CS20180411 [pii]
AID - 10.1042/CS20180411 [doi]
PST - epublish
SO  - Clin Sci (Lond). 2018 Jul 31;132(14):1597-1614. doi: 10.1042/CS20180411. Print 
      2018 Jul 31.