PMID- 29998870 OWN - NLM STAT- MEDLINE DCOM- 20181211 LR - 20220331 IS - 0971-5916 (Print) IS - 0971-5916 (Linking) VI - 147 IP - 4 DP - 2018 Apr TI - Erythrocyte membrane fatty acid profile & serum cytokine levels in patients with non-alcoholic fatty liver disease. PG - 352-360 LID - 10.4103/ijmr.IJMR_1065_16 [doi] AB - BACKGROUND & OBJECTIVES: Fatty acids may affect the expression of genes, and this process is influenced by sex hormones. Cytokines are involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), so this study was aimed to assess the association of erythrocyte membrane fatty acids with three cytokines and markers of hepatic injury in NAFLD patients and to explore whether these associations were the same in both sexes. METHODS: In this cross-sectional study, 62 consecutive patients (32 men and 30 women) with NAFLD during the study period. Tumour necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), aspartate aminotransferase and alanine aminotransferase were measured in a fasting serum sample, and Fibroscan was conducted for each individual. Gas chromatography was used to measure erythrocyte membrane fatty acids. Univariate and multiple linear regressions were used to analyze data. RESULTS: In men, IL-6 had a significant (P <0.05) positive association with total omega-3 polyunsaturated fatty acids (PUFAs). In women, TNF-alpha had a significant positive association with total omega-3 (P <0.05) and omega-6 (P <0.01) PUFAs, IL-6 had a significant (P <0.05) positive association with total monounsaturated fatty acids and MCP-1 had a significant positive association with total trans-fatty acids (P <0.05). No significant associations were observed between erythrocyte membrane fatty acids and liver enzymes or Fibroscan report in both sexes. In this study, women were significantly older than men [51 (42.75-55) vs 35.5 (29-52), P <0.01], so the associations were adjusted for age and other confounders. INTERPRETATION & CONCLUSIONS: Erythrocyte membrane fatty acid profile was not associated with serum liver enzymes or Fibroscan reports in NAFLD patients, but it had significant associations with serum TNF-alpha, IL-6 and MCP-1 and these associations were probably sex dependent. FAU - Amirkalali, Bahareh AU - Amirkalali B AD - Gastrointestinal & Liver Disease Research Center, Iran University of Medical Sciences, Tehran, Iran. FAU - Sohrabi, Masoud Reza AU - Sohrabi MR AD - Gastrointestinal & Liver Disease Research Center, Iran University of Medical Sciences, Tehran, Iran. FAU - Esrafily, Ali AU - Esrafily A AD - Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. FAU - Jalali, Mahmoud AU - Jalali M AD - Department of Nutrition & Biochemistry, School of Nutritional Sciences & Dietetics, Tehran University of Medical Sciences, Tehran, Iran. FAU - Gholami, Ali AU - Gholami A AD - Department of Epidemiology, School of Public Health, Iran University of Medical Sciences, Tehran; Department of Public Health, School of Public Health, Neyshabur University of Medical Sciences, Neyshabur, Iran. FAU - Hosseinzadeh, Payam AU - Hosseinzadeh P AD - Gastrointestinal & Liver Disease Research Center, Iran University of Medical Sciences, Tehran, Iran. FAU - Keyvani, Hossein AU - Keyvani H AD - Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. FAU - Shidfar, Farzad AU - Shidfar F AD - Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. FAU - Zamani, Farhad AU - Zamani F AD - Gastrointestinal & Liver Disease Research Center, Iran University of Medical Sciences, Tehran, Iran. LA - eng PT - Journal Article PL - India TA - Indian J Med Res JT - The Indian journal of medical research JID - 0374701 RN - 0 (Cytokines) RN - 0 (Fatty Acids) RN - 0 (Fatty Acids, Omega-3) SB - IM CIN - Indian J Med Res. 2018 Apr;147(4):334-336. PMID: 29998867 MH - Cross-Sectional Studies MH - Cytokines MH - Erythrocyte Membrane/*chemistry MH - Fatty Acids/*metabolism MH - Fatty Acids, Omega-3 MH - Female MH - Humans MH - Iran MH - Liver MH - Male MH - *Non-alcoholic Fatty Liver Disease PMC - PMC6057244 OTO - NOTNLM OT - Cytokine - erythrocyte membrane - fatty acid - hepatic injury - NAFLD - non-alcoholic fatty liver COIS- None EDAT- 2018/07/13 06:00 MHDA- 2018/12/12 06:00 PMCR- 2018/04/01 CRDT- 2018/07/13 06:00 PHST- 2018/07/13 06:00 [entrez] PHST- 2018/07/13 06:00 [pubmed] PHST- 2018/12/12 06:00 [medline] PHST- 2018/04/01 00:00 [pmc-release] AID - IndianJMedRes_2018_147_4_352_236350 [pii] AID - IJMR-147-352 [pii] AID - 10.4103/ijmr.IJMR_1065_16 [doi] PST - ppublish SO - Indian J Med Res. 2018 Apr;147(4):352-360. doi: 10.4103/ijmr.IJMR_1065_16.