PMID- 30001408
OWN - NLM
STAT- MEDLINE
DCOM- 20190108
LR  - 20190108
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 7
DP  - 2018
TI  - Identification of changes in dendritic cell subsets that correlate with disease 
      severity in dengue infection.
PG  - e0200564
LID - 10.1371/journal.pone.0200564 [doi]
LID - e0200564
AB  - Dengue virus (DENV) is the most prevalent arthropod-borne viral disease in 
      humans. DENV causes a spectrum of illness ranging from mild to potentially severe 
      complications. Dendritic cells (DCs) play a critical role in initiating and 
      regulating highly effective antiviral immune response that include linking innate 
      and adaptive immune responses. This study was conducted to comparatively 
      characterize in detail the relative proportion, phenotypic changes, and 
      maturation profile of subsets of both myeloid DCs (mDCs) and plasmacytoid DCs 
      (pDCs) in children with dengue fever (DF), dengue hemorrhagic fever (DHF) and for 
      purposes of control healthy individuals. The mDCs (Lin-CD11c+CD123lo), the pDCs 
      (Lin-CD11c-CD123+) and the double negative (DN) subset 
      (Lin-/HLA-DR+/CD11c-CD123-) were analyzed by polychromatic flow cytometry. The 
      data were first analyzed on blood samples collected from DENV-infected patients 
      at various times post-infection. Results showed that the relative proportion of 
      mDCs were significantly decreased which was associated with an increase in 
      disease severity in samples from DENV-infected patients. While there was no 
      significant difference in the relative proportion of pDCs between healthy and 
      DENV-infected patients, there was a marked increase in the DN subset. Analysis of 
      the kinetics of changes of pDCs showed that there was an increase but only during 
      the early febrile phase. Additionally, samples from patients during acute disease 
      showed marked decreases in the relative proportion of CD141+ and CD16+ mDC 
      subsets that were the major mDC subsets in healthy individuals. In addition, 
      there was a significant decrease in the level of CD33-expressing mDCs in DENV 
      patients. While the pDCs showed an up-regulation of maturation profile during 
      acute DENV infection, the mDCs showed an alteration of maturation status. This 
      study suggests that different relative proportion and phenotypic changes as well 
      as alteration of maturation profile of DC subsets may play a critical role in the 
      dengue pathogenesis and disease outcome.
FAU - Lertjuthaporn, Sakaorat
AU  - Lertjuthaporn S
AD  - Graduate Program in Immunology, Department of Immunology, Faculty of Medicine 
      Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Khowawisetsut, Ladawan
AU  - Khowawisetsut L
AUID- ORCID: 0000-0003-1585-495X
AD  - Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Bangkok, Thailand.
FAU - Keawvichit, Rassamon
AU  - Keawvichit R
AD  - Graduate Program in Immunology, Department of Immunology, Faculty of Medicine 
      Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Polsrila, Korakot
AU  - Polsrila K
AD  - Department of Research and Development, Faculty of Medicine Siriraj Hospital, 
      Mahidol University, Bangkok, Thailand.
FAU - Chuansumrit, Ampaiwan
AU  - Chuansumrit A
AD  - Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol 
      University, Bangkok, Thailand.
FAU - Chokephaibulkit, Kulkanya
AU  - Chokephaibulkit K
AD  - Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Bangkok, Thailand.
FAU - Thitilertdecha, Premrutai
AU  - Thitilertdecha P
AUID- ORCID: 0000-0002-1853-4073
AD  - Research Group in Immunobiology and Therapeutic Sciences, Faculty of Medicine 
      Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Onlamoon, Nattawat
AU  - Onlamoon N
AD  - Research Group in Immunobiology and Therapeutic Sciences, Faculty of Medicine 
      Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Ansari, Aftab A
AU  - Ansari AA
AD  - Department of Pathology and Laboratory Medicine, Emory University School of 
      Medicine, Atlanta, Georgia, United States of America.
FAU - Pattanapanyasat, Kovit
AU  - Pattanapanyasat K
AUID- ORCID: 0000-0003-1037-6610
AD  - Graduate Program in Immunology, Department of Immunology, Faculty of Medicine 
      Siriraj Hospital, Mahidol University, Bangkok, Thailand.
AD  - Department of Research and Development, Faculty of Medicine Siriraj Hospital, 
      Mahidol University, Bangkok, Thailand.
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180712
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Adolescent
MH  - Antigens, CD/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Dendritic Cells/*immunology/pathology
MH  - Dengue/*immunology/pathology
MH  - Dengue Virus/*immunology
MH  - Female
MH  - Humans
MH  - Male
MH  - Myeloid Cells/*immunology/pathology
PMC - PMC6042784
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/07/13 06:00
MHDA- 2019/01/09 06:00
PMCR- 2018/07/12
CRDT- 2018/07/13 06:00
PHST- 2018/03/15 00:00 [received]
PHST- 2018/06/28 00:00 [accepted]
PHST- 2018/07/13 06:00 [entrez]
PHST- 2018/07/13 06:00 [pubmed]
PHST- 2019/01/09 06:00 [medline]
PHST- 2018/07/12 00:00 [pmc-release]
AID - PONE-D-18-07932 [pii]
AID - 10.1371/journal.pone.0200564 [doi]
PST - epublish
SO  - PLoS One. 2018 Jul 12;13(7):e0200564. doi: 10.1371/journal.pone.0200564. 
      eCollection 2018.