PMID- 30001730
OWN - NLM
STAT- MEDLINE
DCOM- 20190619
LR  - 20191210
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 16
IP  - 1
DP  - 2018 Jul 13
TI  - Tanshinone IIA promotes the differentiation of bone marrow mesenchymal stem cells 
      into neuronal-like cells in a spinal cord injury model.
PG  - 193
LID - 10.1186/s12967-018-1571-y [doi]
LID - 193
AB  - BACKGROUND: Spinal cord injury (SCI) is one of the most severe central nervous 
      system injuries. Currently, transplanting bone marrow mesenchymal stem cells 
      (BMSCs) is considered a therapeutic option for SCI. Tanshinone IIA (TIIA) is one 
      of the extracts obtained from Salvia miltiorrhiza Bunge, which has been shown to 
      have some protective effects against SCI. The present research was aimed to 
      explore whether TIIA would influence the fate of transplanted BMSCs in a rat 
      model of SCI, especially with regard to their differentiation into neuronal 
      cells. METHODS: Bone marrow mesenchymal stem cells were obtained from immature 
      rats and identified using flow cytometry. After SCI, 1.0 x 10(7) cells labeled 
      with PKH67 were transfused into the injured spinal cord. TIIA was first injected 
      into the tail vein (30 mg/kg) 1 h before surgery. From day 1 to day 7 post-SCI, 
      TIIA was injected (20 mg/kg) per day at the same time. Recovery of locomotor 
      function and histological regeneration of the spinal cord were compared among the 
      groups, with the differentiation and distribution of BMSCs determined 
      anatomically and biochemically by the expression of neural cell markers. RESULTS: 
      Locomotor assessments showed that the rats in the BMSCs + TIIA group exhibited 
      higher scores (19.33 +/- 0.58) than those in the other groups (13.67 +/- 1.53, 
      17.67 +/- 0.58, 18.00 +/- 1.73). The area of the cavity in the BMSCs + TIIA rats was 
      smaller than that in the other groups (1.30 +/- 0.56, 10.39 +/- 1.59, 6.84 +/- 1.18, 
      4.36 +/- 0.69). Co-expression of glial fibrillary acid protein was observed in 
      transplanted BMSCs, with a reduced rate in the BMSCs + TIIA group relative to 
      that in the SCI group. In contrast, the expression levels of Nestin, 
      neuron-specific nuclear protein (NeuN) and neurofilament protein 200 (NF200) were 
      greatest in the transplanted cells in the BMSCs + TIIA group. CONCLUSIONS: 
      Tanshinone IIA treatment enhances the therapeutic effects of BMSC transplant on 
      SCI, likely by promoting the differentiation of neuronal cells.
FAU - Zhang, Xue-Mei
AU  - Zhang XM
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Ma, Jiao
AU  - Ma J
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Sun, Yang
AU  - Sun Y
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Yu, Bing-Qian
AU  - Yu BQ
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Jiao, Zhuo-Min
AU  - Jiao ZM
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Wang, Duo
AU  - Wang D
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Yu, Mei-Yu
AU  - Yu MY
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Li, Jin-Yue
AU  - Li JY
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China.
FAU - Fu, Jin
AU  - Fu J
AD  - Department of Neurology, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246 XueFu Road, Nangang District, Harbin, 150001, People's 
      Republic of China. fujin198@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180713
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (Abietanes)
RN  - 0 (Biomarkers)
RN  - 03UUH3J385 (tanshinone)
SB  - IM
MH  - Abietanes/*pharmacology
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Image Processing, Computer-Assisted
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Motor Activity/drug effects
MH  - Myelin Sheath/drug effects/pathology/ultrastructure
MH  - Neuroglia/drug effects/metabolism
MH  - Neurons/*cytology/drug effects
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/drug effects
MH  - Spinal Cord Injuries/pathology/physiopathology/*therapy
PMC - PMC6044071
OTO - NOTNLM
OT  - Bone marrow mesenchymal stem cells
OT  - Differentiation
OT  - Spinal cord injury
OT  - Tanshinone IIA
EDAT- 2018/07/14 06:00
MHDA- 2019/06/20 06:00
PMCR- 2018/07/13
CRDT- 2018/07/14 06:00
PHST- 2018/04/12 00:00 [received]
PHST- 2018/07/07 00:00 [accepted]
PHST- 2018/07/14 06:00 [entrez]
PHST- 2018/07/14 06:00 [pubmed]
PHST- 2019/06/20 06:00 [medline]
PHST- 2018/07/13 00:00 [pmc-release]
AID - 10.1186/s12967-018-1571-y [pii]
AID - 1571 [pii]
AID - 10.1186/s12967-018-1571-y [doi]
PST - epublish
SO  - J Transl Med. 2018 Jul 13;16(1):193. doi: 10.1186/s12967-018-1571-y.