PMID- 30003355
OWN - NLM
STAT- MEDLINE
DCOM- 20190321
LR  - 20190321
IS  - 1613-2246 (Electronic)
IS  - 0021-5155 (Linking)
VI  - 62
IP  - 5
DP  - 2018 Sep
TI  - Frequencies of human leukocyte antigen alleles and haplotypes among Japanese 
      patients with age-related macular degeneration.
PG  - 568-575
LID - 10.1007/s10384-018-0611-8 [doi]
AB  - PURPOSE: Stem cell therapy is a potential treatment for retinal disorders. We are 
      currently exploring treating HLA matched patients of age-related macular 
      degeneration (AMD) by using allogenic retinal pigment epithelium cells derived 
      from induced pluripotent stem cells (iPS-RPE) from human leukocyte antigen (HLA) 
      homozygote donors. The purpose of this study was to investigate the frequency of 
      HLA class I and II alleles and haplotypes in Japanese patients with AMD. STUDY 
      DESIGN: Cross-sectional observation clinical study. METHODS: A total of 138 
      consecutive patients diagnosed with neovascular AMD (mean age, 76.0 +/- 7.8 years, 
      105 men) and 300 controls were included in the study. The frequencies of HLA-A, 
      -B, -C, -DRB1, -DQB1, and -DPB1 alleles were determined using illumina MiSeq 
      platform. Frequencies of HLA alleles at six loci in patients with AMD were 
      compared with those of the controls. RESULTS: The alleles with the highest 
      prevalence at each locus were A*24:02 (29.7%), B*52:01 (15.5%), C*12:02 (16.1%), 
      DRB1*09:01 (19.1%), DQB1*06:01 (23.2%), and DPB1* 05:01 (40.5%). There were no 
      significant associations between the HLA alleles and AMD. The most common 
      haplotype was A*24:02-B*52:01-C*12:02-DRB1*15:02-DQB1*06:01-DPB1*09:01, with a 
      9.8% genetic frequency among all haplotypes, detected in 18.8% of the patients. 
      CONCLUSION: The genotype of HLA in patients with AMD was not different from that 
      in the Japanese control population. Thus, therapy with iPS-RPEof the most 
      frequent HLA haplotype could be a feasible alternative for AMD in a wider 
      population.
FAU - Takagi, Seiji
AU  - Takagi S
AUID- ORCID: 0000-0001-6267-1641
AD  - Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8, 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan. tigerseiji@gmail.com.
AD  - Department of Ophthalmology, Kobe City Medical Center General Hospital, 2-2 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan. tigerseiji@gmail.com.
AD  - Department of Ophthalmology, Toho University Ohashi Medical Center, 2-6-17 
      Ohashi, Meguro-ku, Tokyo, 153-8515, Japan. tigerseiji@gmail.com.
FAU - Mandai, Michiko
AU  - Mandai M
AD  - Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8, 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Department of Ophthalmology, Kobe City Medical Center General Hospital, 2-2 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 
      2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
FAU - Hirami, Yasuhiko
AU  - Hirami Y
AD  - Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8, 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Department of Ophthalmology, Kobe City Medical Center General Hospital, 2-2 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 
      2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
FAU - Sugita, Sunao
AU  - Sugita S
AD  - Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8, 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Department of Ophthalmology, Kobe City Medical Center General Hospital, 2-2 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 
      2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
FAU - Takahashi, Masayo
AU  - Takahashi M
AD  - Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8, 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Department of Ophthalmology, Kobe City Medical Center General Hospital, 2-2 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 
      2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
FAU - Kurimoto, Yasuo
AU  - Kurimoto Y
AD  - Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8, 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Department of Ophthalmology, Kobe City Medical Center General Hospital, 2-2 
      Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 
      2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
LA  - eng
PT  - Journal Article
PT  - Observational Study
DEP - 20180712
PL  - Japan
TA  - Jpn J Ophthalmol
JT  - Japanese journal of ophthalmology
JID - 0044652
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Aged
MH  - Alleles
MH  - Cross-Sectional Studies
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - HLA Antigens/*genetics
MH  - Haplotypes
MH  - Humans
MH  - Incidence
MH  - Japan/epidemiology
MH  - Male
MH  - Retinal Pigment Epithelium/*cytology
MH  - Stem Cell Transplantation/*methods
MH  - *Visual Acuity
MH  - Wet Macular Degeneration/epidemiology/*genetics/therapy
OTO - NOTNLM
OT  - Age-related macular degeneration
OT  - Haplotype frequency
OT  - Human leukocyte antigen
OT  - Induced pluripotent stem cells
OT  - Japan
EDAT- 2018/07/14 06:00
MHDA- 2019/03/22 06:00
CRDT- 2018/07/14 06:00
PHST- 2017/12/04 00:00 [received]
PHST- 2018/06/06 00:00 [accepted]
PHST- 2018/07/14 06:00 [pubmed]
PHST- 2019/03/22 06:00 [medline]
PHST- 2018/07/14 06:00 [entrez]
AID - 10.1007/s10384-018-0611-8 [pii]
AID - 10.1007/s10384-018-0611-8 [doi]
PST - ppublish
SO  - Jpn J Ophthalmol. 2018 Sep;62(5):568-575. doi: 10.1007/s10384-018-0611-8. Epub 
      2018 Jul 12.