PMID- 30003389
OWN - NLM
STAT- MEDLINE
DCOM- 20181231
LR  - 20181231
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 43
IP  - 9
DP  - 2018 Sep
TI  - Association of Induced Hyperhomocysteinemia with Alzheimer's Disease-Like 
      Neurodegeneration in Rat Cortical Neurons After Global Ischemia-Reperfusion 
      Injury.
PG  - 1766-1778
LID - 10.1007/s11064-018-2592-x [doi]
AB  - Alzheimer's disease (AD) is a progressive and irreversible neurodegenerative 
      disorder that results in massive hippocampal and neocortical neuronal loss 
      leading to dementia and eventual death. The exact cause of Alzheimer's disease is 
      not fully explored, although a number of risk factors have been recognized, 
      including high plasma concentration of homocysteine (Hcy). Hyperhomocysteinemia 
      (hHcy) is considered a strong, independent risk factor for stroke and dementia. 
      However, the molecular background underlying these mechanisms linked with hHcy 
      and ischemic stroke is not fully understood. Paper describes rat model of global 
      forebrain ischemia combined with the experimentally induced hHcy. Global 
      ischemia-reperfusion injury (IRI) was developed by 4-vessels occlusion lasting 
      for 15 min followed by reperfusion period of 72 h. hHcy was induced by 
      subcutaneous injection of 0.45 micromol/g of Hcy in duration of 14 days. The results 
      showed remarkable neural cell death induced by hHcy in the brain cortex and 
      neurodegeneration is further aggravated by global IRI. We demonstrated 
      degeneration of cortical neurons, alterations in number and morphology of tissue 
      astrocytes and dysregulation of oxidative balance with increased membrane protein 
      oxidation. Complementary to, an immunohistochemical analysis of tau protein and 
      beta-amyloid peptide showed that combination of hHcy with the IRI might lead to the 
      progression of AD-like pathological features. Conclusively, these findings 
      suggest that combination of risk factor hHcy with IRI aggravates 
      neurodegeneration processes and leads to development of AD-like pathology in 
      cerebral cortex.
FAU - Kovalska, Maria
AU  - Kovalska M
AD  - Jessenius Faculty of Medicine in Martin, Institute of Histology and Embryology, 
      Comenius University in Bratislava, Martin, Slovakia.
FAU - Tothova, Barbara
AU  - Tothova B
AD  - BioMed, Division of Oncology, Jessenius Faculty of Medicine in Martin, Comenius 
      University in Bratislava, Martin, Slovakia.
AD  - Jessenius Faculty of Medicine in Martin, Institute of Medical Biochemistry, 
      Comenius University in Bratislava, Martin, Slovakia.
FAU - Kovalska, Libusa
AU  - Kovalska L
AD  - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 
      Clinic of Stomatology and Maxillofacial Surgery, Martin, Slovakia.
FAU - Tatarkova, Zuzana
AU  - Tatarkova Z
AD  - Jessenius Faculty of Medicine in Martin, Institute of Medical Biochemistry, 
      Comenius University in Bratislava, Martin, Slovakia.
FAU - Kalenska, Dagmar
AU  - Kalenska D
AD  - Jessenius Faculty of Medicine in Martin, Institute of Medical Biochemistry, 
      Comenius University in Bratislava, Martin, Slovakia.
FAU - Tomascova, Anna
AU  - Tomascova A
AD  - Jessenius Faculty of Medicine in Martin, Institute of Medical Biochemistry, 
      Comenius University in Bratislava, Martin, Slovakia.
FAU - Adamkov, Marian
AU  - Adamkov M
AD  - Jessenius Faculty of Medicine in Martin, Institute of Histology and Embryology, 
      Comenius University in Bratislava, Martin, Slovakia.
FAU - Lehotsky, Jan
AU  - Lehotsky J
AUID- ORCID: 0000-0002-2408-5745
AD  - Jessenius Faculty of Medicine in Martin, Institute of Medical Biochemistry, 
      Comenius University in Bratislava, Martin, Slovakia. lehotsky@jfmed.uniba.sk.
AD  - BioMed, Division of Neurosciences, Jessenius Faculty of Medicine in Martin, 
      Comenius University in Bratislava, Martin, Slovakia. lehotsky@jfmed.uniba.sk.
AD  - Jessenius Faculty of Medicine, Institute of Medical Biochemistry and BioMed, 
      Comenius University, Mala Hora 4D, 036 01, Martin, Slovakia. 
      lehotsky@jfmed.uniba.sk.
LA  - eng
GR  - VEGA 128/16/Slovak Grant Agency/
GR  - 15/0107/Agentura na Podporu Vyskumu a Vyvoja/
PT  - Journal Article
DEP - 20180712
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0LVT1QZ0BA (Homocysteine)
SB  - IM
MH  - Alzheimer Disease/chemically induced/*pathology
MH  - Animals
MH  - Cerebral Cortex/drug effects/*pathology
MH  - Homocysteine/*toxicity
MH  - Hyperhomocysteinemia/chemically induced/*pathology
MH  - Male
MH  - Nerve Degeneration/chemically induced/*pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/chemically induced/*pathology
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Brain ischemia
OT  - Homocysteine
OT  - beta-Amyloid peptide
EDAT- 2018/07/14 06:00
MHDA- 2019/01/01 06:00
CRDT- 2018/07/14 06:00
PHST- 2018/03/16 00:00 [received]
PHST- 2018/07/05 00:00 [accepted]
PHST- 2018/06/25 00:00 [revised]
PHST- 2018/07/14 06:00 [pubmed]
PHST- 2019/01/01 06:00 [medline]
PHST- 2018/07/14 06:00 [entrez]
AID - 10.1007/s11064-018-2592-x [pii]
AID - 10.1007/s11064-018-2592-x [doi]
PST - ppublish
SO  - Neurochem Res. 2018 Sep;43(9):1766-1778. doi: 10.1007/s11064-018-2592-x. Epub 
      2018 Jul 12.