PMID- 30004605 OWN - NLM STAT- MEDLINE DCOM- 20191127 LR - 20231213 IS - 1549-4918 (Electronic) IS - 1066-5099 (Linking) VI - 36 IP - 10 DP - 2018 Oct TI - Repurposing the Cord Blood Bank for Haplobanking of HLA-Homozygous iPSCs and Their Usefulness to Multiple Populations. PG - 1552-1566 LID - 10.1002/stem.2865 [doi] AB - Although autologous induced pluripotent stem cells (iPSCs) can potentially be useful for treating patients without immune rejection, in reality it will be extremely expensive and labor-intensive to make iPSCs to realize personalized medicine. An alternative approach is to make use of human leukocyte antigen (HLA) haplotype homozygous donors to provide HLA matched iPSC products to significant numbers of patients. To establish a haplobank of iPSCs, we repurposed the cord blood bank by screening approximately 4,200 high resolution HLA typed cord blood samples, and selected those homozygous for the 10 most frequent HLA-A,-B,-DRB1 haplotypes in the Korean population. Following the generation of 10 iPSC lines, we conducted a comprehensive characterization, including morphology, expression of pluripotent markers and cell surface antigens, three-germ layer formation, vector clearance, mycoplasma/microbiological/viral contamination, endotoxin, and short tandem repeat (STR) assays. Various genomic analyses using microarray and comparative genomic hybridization (aCGH)-based single nucleotide polymorphism (SNP) and copy number variation (CNV) were also conducted. These 10 HLA-homozygous iPSC lines match 41.07% of the Korean population. Comparative analysis of HLA population data shows that they are also of use in other Asian populations, such as Japan, with some limited utility in ethnically diverse populations, such as the UK. Taken together, the generation of the 10 most frequent Korean HLA-homozygous iPSC lines serves as a useful pointer for the development of optimal methods for iPSC generation and quality control and indicates the benefits and limitations of collaborative HLA driven selection of donors for future stocking of worldwide iPSC haplobanks. Stem Cells 2018;36:1552-1566. CI - (c) 2018 The Authors Stem Cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. FAU - Lee, Suji AU - Lee S AD - Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea. FAU - Huh, Ji Young AU - Huh JY AD - Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea. FAU - Turner, David M AU - Turner DM AD - Histocompatibility and Immunogenetics Laboratory, Royal Infirmary of Edinburgh, Edinburgh, UK. AD - Advanced Therapeutics, Scottish National Blood Transfusion Service, Edinburgh, UK. FAU - Lee, Soohyeon AU - Lee S AD - Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea. FAU - Robinson, James AU - Robinson J AD - HLA Informatics Group, Anthony Nolan Research Institute, Royal Free Campus, London, UK. AD - UCL Cancer Institute, University College London, London, UK. FAU - Stein, Jeremy E AU - Stein JE AD - HLA Informatics Group, Anthony Nolan Research Institute, Royal Free Campus, London, UK. FAU - Shim, Sung Han AU - Shim SH AD - Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea. FAU - Hong, Chang Pyo AU - Hong CP AD - Bioinformatics Team, Theragen Etex Bio Institute, Suwon-si, Gyeonggi-do, Republic of Korea. FAU - Kang, Myung Seo AU - Kang MS AD - Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea. FAU - Nakagawa, Masato AU - Nakagawa M AD - Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. FAU - Kaneko, Shin AU - Kaneko S AD - Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. FAU - Nakanishi, Mahito AU - Nakanishi M AD - Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan. FAU - Rao, Mahendra S AU - Rao MS AD - New York Stem Cell Foundation Research Institute, New York, New York, USA. FAU - Kurtz, Andreas AU - Kurtz A AD - Berlin-Brandenburg Center for Regenerative Therapies, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Stacey, Glyn N AU - Stacey GN AD - International Stem Cell Banking Initiative, Hertfordshire, UK. FAU - Marsh, Steven G E AU - Marsh SGE AD - HLA Informatics Group, Anthony Nolan Research Institute, Royal Free Campus, London, UK. AD - UCL Cancer Institute, University College London, London, UK. FAU - Turner, Marc L AU - Turner ML AD - Advanced Therapeutics, Scottish National Blood Transfusion Service, Edinburgh, UK. AD - Global Alliance for iPSC Therapies, The Jack Copland Centre, Edinburgh, UK. FAU - Song, Jihwan AU - Song J AUID- ORCID: 0000-0002-6597-8208 AD - Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea. AD - Global Alliance for iPSC Therapies, The Jack Copland Centre, Edinburgh, UK. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180729 PL - England TA - Stem Cells JT - Stem cells (Dayton, Ohio) JID - 9304532 RN - 0 (HLA Antigens) RN - 0 (Histocompatibility Antigens Class II) SB - IM MH - Blood Banking/*methods MH - Genomic Instability/*genetics MH - HLA Antigens/*metabolism MH - Haplotypes MH - Histocompatibility Antigens Class II MH - Humans MH - Induced Pluripotent Stem Cells/*metabolism OTO - NOTNLM OT - Cord blood banks OT - Genomic stability OT - Haplobanking OT - Human leukocyte antigen OT - Immune rejection OT - Induced pluripotent stem cells EDAT- 2018/07/14 06:00 MHDA- 2019/11/28 06:00 CRDT- 2018/07/14 06:00 PHST- 2018/02/01 00:00 [received] PHST- 2018/06/17 00:00 [revised] PHST- 2018/05/02 00:00 [accepted] PHST- 2018/07/14 06:00 [pubmed] PHST- 2019/11/28 06:00 [medline] PHST- 2018/07/14 06:00 [entrez] AID - 10.1002/stem.2865 [doi] PST - ppublish SO - Stem Cells. 2018 Oct;36(10):1552-1566. doi: 10.1002/stem.2865. Epub 2018 Jul 29.