PMID- 30004652
OWN - NLM
STAT- MEDLINE
DCOM- 20180731
LR  - 20180731
IS  - 1000-8721 (Print)
IS  - 1000-8721 (Linking)
VI  - 32
IP  - 6
DP  - 2016 Nov
TI  - [Isolation and Identification of the Nam Dinh Virus from Mosquitoes on the 
      China-Laos-Myanmar Border].
PG  - 782-9
AB  - Three strains of an insect nidovirus, the Nam Dinh virus (NDiV), isolated in 
      Yunnan Province, China, have been identified. Aedes albopictus C6/36 cells were 
      used to isolate NDiV from mosquitoes collected in Yunnan Province in 2012.Culture 
      supernatants with a positive cytopathic effect were harvested for virus 
      identification by sequence-independent single primer amplification. Transmission 
      electron microscopy revealed virion structure to be spherical with a diameter of 
      60~80nm.Reverse transcription-polymerase chain reaction was applied to amplify 
      sequences of RNA-dependent RNA-polymerase (RdRp), HEL1(superfamily 1helicase)and 
      spike protein. The amino-acid sequences of three isolates from Yunnan Province 
      showed>98% homology with NDiV strains. Phylogenetic analyses showed that these 
      three isolates, along with NDiV, could be classified into the family 
      Mesoniviridae.
FAU - Lei, Wenwen
AU  - Lei W
FAU - Guo, Xiaofang
AU  - Guo X
FAU - Fu, Shihong
AU  - Fu S
FAU - Feng, Yun
AU  - Feng Y
FAU - Song, Jingdong
AU  - Song J
FAU - Zhou, Hongning
AU  - Zhou H
FAU - Liang, Guodong
AU  - Liang G
LA  - chi
PT  - Journal Article
PL  - China
TA  - Bing Du Xue Bao
JT  - Bing du xue bao = Chinese journal of virology
JID - 8803009
SB  - IM
MH  - Animals
MH  - China
MH  - Culicidae/classification/*virology
MH  - Humans
MH  - Laos
MH  - Mosquito Vectors/classification/*virology
MH  - Nidovirales/classification/genetics/*isolation & purification/ultrastructure
MH  - Nidovirales Infections/transmission/*virology
MH  - Phylogeny
EDAT- 2016/11/01 00:00
MHDA- 2018/08/01 06:00
CRDT- 2018/07/14 06:00
PHST- 2018/07/14 06:00 [entrez]
PHST- 2016/11/01 00:00 [pubmed]
PHST- 2018/08/01 06:00 [medline]
PST - ppublish
SO  - Bing Du Xue Bao. 2016 Nov;32(6):782-9.