PMID- 30007425
OWN - NLM
STAT- MEDLINE
DCOM- 20190327
LR  - 20190327
IS  - 1878-6146 (Print)
VI  - 122
IP  - 8
DP  - 2018 Aug
TI  - Characterization of extracellular proteins in members of the Paracoccidioides 
      complex.
PG  - 738-751
LID - S1878-6146(18)30062-X [pii]
LID - 10.1016/j.funbio.2018.04.001 [doi]
AB  - Paracoccidioides is a thermodimorphic fungus that causes Paracoccidioidomycosis 
      (PCM) - an endemic systemic mycosis in Latin America. The genus comprises several 
      phylogenetic species which present some genetic and serological differences. The 
      diversity presented among isolates of the same genus has been explored in several 
      microorganisms. There have also been attempts to clarify differences that might 
      be related to virulence existing in isolates that cause the same disease. In this 
      work, we analyzed the secretome of two isolates in the Paracoccidioides genus, 
      isolates Pb01 and PbEpm83, and performed infection assays in macrophages to 
      evaluate the influence of the secretomes of those isolates upon an in vitro model 
      of infection. The use of a label-free proteomics approach (LC-MS(E)) allowed us 
      to identify 92 proteins that are secreted by those strains. Of those proteins, 35 
      were differentially secreted in Pb01, and 36 in PbEpm83. According to the 
      functional annotation, most of the identified proteins are related to adhesion 
      and virulence processes. These results provide evidence that different members of 
      the Paracoccidioides complex can quantitatively secrete different proteins, which 
      may influence the characteristics of virulence, as well as host-related 
      processes.
CI  - Copyright (c) 2018 British Mycological Society. Published by Elsevier Ltd. All 
      rights reserved.
FAU - de Oliveira, Amanda Rodrigues
AU  - de Oliveira AR
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Oliveira, Lucas Nojosa
AU  - Oliveira LN
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil; Programa de Pos-graduacao em Patologia 
      Molecular, Faculdade de Medicina, Universidade de Brasilia, Brasilia, Distrito 
      Federal, Brazil.
FAU - Chaves, Edilania Gomes Araujo
AU  - Chaves EGA
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Weber, Simone Schneider
AU  - Weber SS
AD  - Instituto de Ciencias Exatas e Tecnologia, Universidade Federal do Amazonas, 
      Itacoatiara, Amazonas, Brazil; Faculdade de Ciencias Farmaceuticas, Alimentos e 
      Nutricao, Universidade Federal do Mato Grosso do Sul, Campo Grande, Mato Grosso 
      do Sul, Brazil.
FAU - Bailao, Alexandre Melo
AU  - Bailao AM
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Parente-Rocha, Juliana Alves
AU  - Parente-Rocha JA
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Baeza, Lilian Cristiane
AU  - Baeza LC
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - de Almeida Soares, Celia Maria
AU  - de Almeida Soares CM
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Borges, Clayton Luiz
AU  - Borges CL
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil. Electronic address: clbluiz2@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180411
PL  - Netherlands
TA  - Fungal Biol
JT  - Fungal biology
JID - 101524465
RN  - 0 (Fungal Proteins)
RN  - 0 (Proteome)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromatography, Liquid
MH  - Fungal Proteins/*analysis
MH  - Latin America
MH  - Macrophages/microbiology
MH  - Mass Spectrometry
MH  - Mice
MH  - Models, Biological
MH  - Paracoccidioides/*chemistry/growth & development/isolation & purification
MH  - Paracoccidioidomycosis/microbiology
MH  - Proteome/*analysis
MH  - Virulence Factors/analysis
OTO - NOTNLM
OT  - Label-free proteomics
OT  - Macrophage infection
OT  - Paracoccidioides species
OT  - Secretome
OT  - Virulence factors
EDAT- 2018/07/17 06:00
MHDA- 2019/03/28 06:00
CRDT- 2018/07/16 06:00
PHST- 2017/11/24 00:00 [received]
PHST- 2018/03/11 00:00 [revised]
PHST- 2018/04/03 00:00 [accepted]
PHST- 2018/07/16 06:00 [entrez]
PHST- 2018/07/17 06:00 [pubmed]
PHST- 2019/03/28 06:00 [medline]
AID - S1878-6146(18)30062-X [pii]
AID - 10.1016/j.funbio.2018.04.001 [doi]
PST - ppublish
SO  - Fungal Biol. 2018 Aug;122(8):738-751. doi: 10.1016/j.funbio.2018.04.001. Epub 
      2018 Apr 11.