PMID- 30008849
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 16
IP  - 2
DP  - 2018 Aug
TI  - Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 
      and the GJIC function in gastric epithelial cells and their association by 
      promoter analysis.
PG  - 1650-1658
LID - 10.3892/ol.2018.8796 [doi]
AB  - The present study aimed to explore the effects of Helicobacter pylori (H. pylori) 
      infection on the expression of transcription factor GATA binding protein 3 
      (GATA-3) and connexin 32 (Cx32) in cultured gastric mucosa cells, and their 
      association with each other. GES-1 cells were co-cultured with East Asian type 
      cytotoxin-associated gene A(+) H. pylori in the H. pylori group, and without H. 
      pylori culture in the control group. Additionally, Mongolian gerbils were gavaged 
      with H. pylori, and later the gastric antrum tissues were collected. The GATA-3 
      and Cx32 mRNA and protein expression levels were detected by a reverse 
      transcription-quantitative polymerase chain reaction and western blot analysis, 
      respectively. The scratch labeling fluorescent dye tracer (SLDT) technique was 
      used to detect the gap junctional intercellular communication (GJIC) function. 
      GATA-3 small interfering RNA (siRNA) was transfected into BGC823 cells and its 
      effect on Cx32 expression levels was detected. The impact of GATA-3 on Cx32 
      promoter transcriptional activity was detected using a dual luciferase reporter 
      assay. The results revealed that H. pylori infection increased GATA-3 expression 
      and decreased Cx32 expression in GES-1 cells and in animal gastric tissues 
      compared with their respective controls, whilst in BGC823 cells, GATA-3 siRNA 
      increased Cx32 expression compared with the control. In the SLDT experiment of 
      GES-1 cells with H. pylori infection, the fluorescent dye was primarily limited 
      to a single cell row close to the scratch, and only a limited amount of dye 
      passing to the second cell row, indicating that the GJIC function was 
      substantially reduced or absent compared with the control group, where the 
      fluorescence dye transferred to the neighboring cells of 3-4 rows, indicating a 
      stronger GJIC function comparatively. GATA-3 inhibited the expression of the 
      luciferase reporter gene, compared with the controls, suggesting that GATA-3 
      inhibited the expression of Cx32 by binding to Cx32 promoter sites. These results 
      indicated that H. pylori-increased GATA-3 expression, which downregulated Cx32 
      expression, may serve an important function in gastric carcinogenesis, and GATA-3 
      siRNA may serve a function in the prevention and treatment of gastric cancer.
FAU - Huang, Lihua
AU  - Huang L
AD  - Center for Medical Experiments, Third Xiangya Hospital of Central South 
      University, Changsha, Hunan 410013, P.R. China.
FAU - Guo, Yinjie
AU  - Guo Y
AD  - Department of Gastroenterology, Third Xiangya Hospital of Central South 
      University, Changsha, Hunan 410013, P.R. China.
FAU - Cao, Dan
AU  - Cao D
AD  - Department of Gastroenterology, Third Xiangya Hospital of Central South 
      University, Changsha, Hunan 410013, P.R. China.
FAU - Liu, Xiaoming
AU  - Liu X
AD  - Department of Gastroenterology, Third Xiangya Hospital of Central South 
      University, Changsha, Hunan 410013, P.R. China.
FAU - Zhang, Linfang
AU  - Zhang L
AD  - Department of Gastroenterology, Third Xiangya Hospital of Central South 
      University, Changsha, Hunan 410013, P.R. China.
FAU - Cao, Ke
AU  - Cao K
AD  - Department of Oncology, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Hu, Tingzi
AU  - Hu T
AD  - Department of Gastroenterology, Third Xiangya Hospital of Central South 
      University, Changsha, Hunan 410013, P.R. China.
FAU - Qi, Yong
AU  - Qi Y
AD  - Clinical Laboratory, Third Xiangya Hospital of Central South University, 
      Changsha, Hunan 410013, P.R. China.
FAU - Xu, Canxia
AU  - Xu C
AD  - Department of Gastroenterology, Third Xiangya Hospital of Central South 
      University, Changsha, Hunan 410013, P.R. China.
AD  - Department of Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Third 
      Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180524
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6036278
OTO - NOTNLM
OT  - Helicobacter pylori
OT  - connexin 32
OT  - gap junctional intercellular communication
OT  - gene promoter
EDAT- 2018/07/17 06:00
MHDA- 2018/07/17 06:01
PMCR- 2018/05/24
CRDT- 2018/07/17 06:00
PHST- 2016/07/06 00:00 [received]
PHST- 2018/03/29 00:00 [accepted]
PHST- 2018/07/17 06:00 [entrez]
PHST- 2018/07/17 06:00 [pubmed]
PHST- 2018/07/17 06:01 [medline]
PHST- 2018/05/24 00:00 [pmc-release]
AID - OL-0-0-8796 [pii]
AID - 10.3892/ol.2018.8796 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Aug;16(2):1650-1658. doi: 10.3892/ol.2018.8796. Epub 2018 May 
      24.