PMID- 30013731
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220318
IS  - 2005-9159 (Print)
IS  - 2093-0569 (Electronic)
IS  - 2005-9159 (Linking)
VI  - 31
IP  - 3
DP  - 2018 Jul
TI  - Orexin-A inhibits capsaicin-induced changes in cyclooxygenase-2 and brain-derived 
      neurotrophic factor expression in trigeminal nucleus caudalis of rats.
PG  - 174-182
LID - 10.3344/kjp.2018.31.3.174 [doi]
AB  - BACKGROUND: The trigeminal nucleus caudalis (Vc) is a primary central site for 
      trigeminal transmitting. Noxious stimulation of the trigeminal nociceptors alters 
      the central synaptic releases and neural expression of some inflammatory and 
      trophic agents. Orexin-A and the orexin 1 receptor (OX1R) are expressed in pain 
      pathways including trigeminal pain transmission. However, the the mechanism(s) 
      underling orexin-A effects on trigeminal pain modulation have not been fully 
      clarified. METHODS: Trigeminal pain was induced by subcutaneous injection of 
      capsaicin in the upper lip in rats. The effect of trigeminal pain on 
      cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) expression 
      in the Vc of animals was determined by immunofluorescence. Subsequently, OX1R 
      agonist (orexin-A) and antagonist (SB-334867-A) was administrated in the Vc to 
      investigate the possible roles of the Vc OX1R on changes in COX-2 and BDNF levels 
      following pain induction. RESULTS: The data indicated an increase in COX-2 and 
      decrease in BDNF immuno-reactivity in the Vc of capsaicin, and capsaicin- 
      pretreated with SB-334867-A (80 nM), groups of rat. However, the effect of 
      capsaicin on COX-2 and BDNF expressions was reversed by a Vc microinjection of 
      orexin-A (100 pM). CONCLUSIONS: Overall, the present data reveals that orexin-A 
      can attenuate capsaicin-induced trigeminal pain through the modulation of pain 
      effects on COX-2 and BDNF expressions in the Vc of rats.
FAU - Kooshki, Razieh
AU  - Kooshki R
AD  - Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, 
      Kerman, Iran.
FAU - Abbasnejad, Mehdi
AU  - Abbasnejad M
AD  - Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, 
      Kerman, Iran.
FAU - Esmaeili Mahani, Saeed
AU  - Esmaeili Mahani S
AD  - Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, 
      Kerman, Iran.
FAU - Raoof, Maryam
AU  - Raoof M
AD  - Endodontology Research Center, Kerman University of Medical Sciences, Kerman, 
      Iran.
AD  - Laboratory of Molecular Neuroscience, Neuroscience Research Center, Institute of 
      Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
FAU - Moeini Aghtaei, Mohammad Mehdi
AU  - Moeini Aghtaei MM
AD  - Pathology and Stem Cell Research Center, Department of Pathology, Afzalipour 
      Kerman University of Medical Science, Kerman, Iran.
FAU - Dabiri, Shahriar
AU  - Dabiri S
AD  - Pathology and Stem Cell Research Center, Department of Pathology, Afzalipour 
      Kerman University of Medical Science, Kerman, Iran.
LA  - eng
PT  - Journal Article
DEP - 20180702
PL  - Korea (South)
TA  - Korean J Pain
JT  - The Korean journal of pain
JID - 101528125
PMC - PMC6037813
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor (BDRF)
OT  - Capsaicin
OT  - Cyclooxygenase 2 (COX 2)
OT  - Orexin receptor antagonists
OT  - Orexin-A
OT  - Orofacial pain
OT  - Pain measurement
OT  - Pain perception
OT  - Rats
OT  - Trigeminal caudal nucleus
OT  - Trigeminal neuralgia
COIS- CONFLICTS OF INTEREST: The authors declared that they have no conflict to 
      interest.
EDAT- 2018/07/18 06:00
MHDA- 2018/07/18 06:01
PMCR- 2018/07/01
CRDT- 2018/07/18 06:00
PHST- 2018/03/30 00:00 [received]
PHST- 2018/06/03 00:00 [revised]
PHST- 2018/06/12 00:00 [accepted]
PHST- 2018/07/18 06:00 [entrez]
PHST- 2018/07/18 06:00 [pubmed]
PHST- 2018/07/18 06:01 [medline]
PHST- 2018/07/01 00:00 [pmc-release]
AID - 10.3344/kjp.2018.31.3.174 [doi]
PST - ppublish
SO  - Korean J Pain. 2018 Jul;31(3):174-182. doi: 10.3344/kjp.2018.31.3.174. Epub 2018 
      Jul 2.