PMID- 30014638
OWN - NLM
STAT- MEDLINE
DCOM- 20190212
LR  - 20190215
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 49
IP  - 3
DP  - 2018 May
TI  - [Effects of Thapsigargin on Intracellular Ca(2+) and Caspase-3 Protein Expression 
      in Mouse Lung Fibroblast].
PG  - 380-383
AB  - OBJECTIVE: To explore the effects of calcium pump inhibitor thapsigargin (TG) on 
      intracellular Ca(2+) and the expression of caspase-3 protein in mouse lung 
      fibroblast. METHODS: Mice lung fibroblast cells were divided into three groups: 
      the blank control group,transforming growth factor-beta(1) (TGF-beta(1)) group and TG 
      treated group. The cells were induced by 5 ng/mL TGF-beta(1) for 24 h in TGF-beta(1) 
      group and TG group,4 mumol/L TG was added in TG group for 24 h. After 48 h,the 
      cells were collected,and the cell structure was observed by transmission electron 
      microscope,intracellular Ca(2+) level was detected with laser confocal 
      microscope,the protein expression of caspase-3 was examined using 
      immunohistochemistry method. RESULTS: Transmission electron microscopy showed 
      that the cells in the blank control group had obvious nucleolus,complete 
      organelles and less apoptosis. In TGF-beta(1) group,the cell morphology was 
      intact,chromatin was evenly distributed,and no apoptotic cells were found. In TG 
      group,there were a large number of apoptosis of fibroblasts,chromatin clumps in 
      nuclei and a small amount of collagen fibers. The level of Ca(2+) in TGF-beta(1) 
      group was significantly lower than that in control group (P<0.05),and which in TG 
      group was significantly higher (P<0.05 ). The protein expression of caspase-3 in 
      TGF-beta(1) group were significantly lower than that in blank control group 
      (P<0.05),which in TG group increased obviously (P<0.05). CONCLUSION: TG could 
      cause intracellular calcium dysregulation in mouse lung fibroblasts,increase 
      caspase-3 protein expression and promote cell apoptosis.
CI  - Copyright(c) by Editorial Board of Journal of Sichuan University (Medical Science 
      Edition).
FAU - Yan, Yu
AU  - Yan Y
AD  - College of Clinical Medicine,North China University of Science and 
      Technology,Tangshan 063000,China.
FAU - Xiao, Kun
AU  - Xiao K
AD  - College of Public Health,North China University of Science and 
      Technology,Tangshan 063000,China.
FAU - Liang, Xiao-Fei
AU  - Liang XF
AD  - College of Public Health,North China University of Science and 
      Technology,Tangshan 063000,China.
FAU - Zhang, Meng-Rou
AU  - Zhang MR
AD  - College of Clinical Medicine,North China University of Science and 
      Technology,Tangshan 063000,China.
FAU - Mao, Kai-Xuan
AU  - Mao KX
AD  - College of Clinical Medicine,North China University of Science and 
      Technology,Tangshan 063000,China.
FAU - Feng, Fan
AU  - Feng F
AD  - College of Clinical Medicine,North China University of Science and 
      Technology,Tangshan 063000,China.
FAU - Xiao, Yong-Hong
AU  - Xiao YH
AD  - College of Public Health,North China University of Science and 
      Technology,Tangshan 063000,China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Calcium/metabolism
MH  - Caspase 3/*metabolism
MH  - Cells, Cultured
MH  - Fibroblasts/*drug effects
MH  - Lung/cytology
MH  - Mice
MH  - RNA, Messenger
MH  - Thapsigargin/*pharmacology
MH  - Transforming Growth Factor beta1/pharmacology
OTO - NOTNLM
OT  - Calcium
OT  - Caspase-3
OT  - Lung fibroblasts
OT  - Thapsigargin
EDAT- 2018/07/18 06:00
MHDA- 2019/02/13 06:00
CRDT- 2018/07/18 06:00
PHST- 2018/07/18 06:00 [entrez]
PHST- 2018/07/18 06:00 [pubmed]
PHST- 2019/02/13 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 May;49(3):380-383.