PMID- 30015261
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1879-3460 (Electronic)
IS  - 0168-1605 (Linking)
VI  - 285
DP  - 2018 Nov 20
TI  - Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier 
      Transform Infrared Spectroscopy: A comprehensive analysis.
PG  - 34-41
LID - S0168-1605(18)30346-5 [pii]
LID - 10.1016/j.ijfoodmicro.2018.07.005 [doi]
AB  - Simpler, quick and low-cost methods for routine Salmonella enterica typing are 
      required for epidemiologic surveillance of this important zoonotic pathogen. In 
      this study, using a comprehensive isolate collection, we investigated the 
      potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the 
      most clinically-relevant serogroups and serotypes of non-typhoid Salmonella. 
      Moreover, the role of O-units composition on the FTIRS Salmonella discrimination 
      was also explored. S. enterica isolates (n = 325; 2002-2015; different sources 
      and countries), of 57 serotypes and 15 serogroups [including the most frequent 
      ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS. 
      Infrared spectra were analysed by Partial Least Square Discriminant Analysis 
      (PLSDA) and/or Principal Component Analysis (PCA). The polysaccharides region 
      provided the spectral sharpest differences being used in the subsequent 
      Salmonella typing. Serogroups (B, C, D and E) discrimination was achieved with 
      high accuracy (99.6% of correct assignments; PLSDA model). Differences in the 
      O-unit structures composition of those serogroups are likely justifying the 
      discrimination achieved. Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were 
      correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 
      isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2). In fact, 
      O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 
      with the remaining serogroups presenting marked differences in this cellular 
      component. The sub-serogroups discrimination was successfully achieved for C1, C2 
      and C3 (using PCA), and for E1-E2-E3 and E4 (by PLSDA). Appropriate serotype 
      discrimination was obtained for most of S. Rissen from the remaining C1 serotypes 
      (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes 
      (93.4%-PLSDA). The lack of available O-unit composition for particular serotypes 
      prevents the elucidation of the role of this cellular component on the 
      discrimination at serotype level obtained. FTIRS was able to discriminate 
      relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and 
      E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. 
      Senftenberg). Further studies on O-antigen composition would clarify the 
      fundaments of discrimination obtained by FTIRS.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Campos, Joana
AU  - Campos J
AD  - UCIBIO/REQUIMTE, Laboratorio de Microbiologia, Faculdade de Farmacia, 
      Universidade do Porto, Portugal.
FAU - Sousa, Clara
AU  - Sousa C
AD  - LAQV/REQUIMTE, Departamento de Ciencias Quimicas, Faculdade de Farmacia, 
      Universidade do Porto, Portugal.
FAU - Mourao, Joana
AU  - Mourao J
AD  - UCIBIO/REQUIMTE, Laboratorio de Microbiologia, Faculdade de Farmacia, 
      Universidade do Porto, Portugal.
FAU - Lopes, Joao
AU  - Lopes J
AD  - Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmacia, 
      Universidade de Lisboa, Lisboa, Portugal.
FAU - Antunes, Patricia
AU  - Antunes P
AD  - UCIBIO/REQUIMTE, Laboratorio de Microbiologia, Faculdade de Farmacia, 
      Universidade do Porto, Portugal; Faculdade de Ciencias da Nutricao e Alimentacao, 
      Universidade do Porto, Portugal.
FAU - Peixe, Luisa
AU  - Peixe L
AD  - UCIBIO/REQUIMTE, Laboratorio de Microbiologia, Faculdade de Farmacia, 
      Universidade do Porto, Portugal. Electronic address: lpeixe@ff.up.pt.
LA  - eng
PT  - Journal Article
DEP - 20180710
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (O Antigens)
SB  - IM
MH  - O Antigens/chemistry
MH  - Salmonella enterica/*classification
MH  - Salmonella enteritidis/*classification
MH  - Serogroup
MH  - Serotyping/*methods
MH  - *Spectroscopy, Fourier Transform Infrared
OTO - NOTNLM
OT  - Bacterial identification methods
OT  - Fourier Transform Infrared Spectroscopy-Attenuated Total Reflectance
OT  - Multivariate data analysis
OT  - O-antigen
OT  - Salmonella enterica
OT  - Typing
EDAT- 2018/07/18 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/07/18 06:00
PHST- 2018/03/19 00:00 [received]
PHST- 2018/05/30 00:00 [revised]
PHST- 2018/07/03 00:00 [accepted]
PHST- 2018/07/18 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/07/18 06:00 [entrez]
AID - S0168-1605(18)30346-5 [pii]
AID - 10.1016/j.ijfoodmicro.2018.07.005 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 2018 Nov 20;285:34-41. doi: 
      10.1016/j.ijfoodmicro.2018.07.005. Epub 2018 Jul 10.