PMID- 30015425
OWN - NLM
STAT- MEDLINE
DCOM- 20190606
LR  - 20190606
IS  - 1733-1331 (Print)
IS  - 1733-1331 (Linking)
VI  - 67
IP  - 1
DP  - 2018 Mar 9
TI  - A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic 
      Microbes.
PG  - 59-65
LID - 10.5604/01.3001.0011.6144 [doi]
AB  - Ureolysis-driven microbially induced carbonate precipitation (MICP) has recently 
      received attention for its potential biotechnological applications. However, 
      information on the enrichment and production of ureolytic microbes by using 
      bioreactor systems is limited. Here, we report a low-tech down-flow hanging 
      sponge (DHS) bioreactor system for the enrichment and production of ureolytic 
      microbes. Using this bioreactor system and a yeast extract-based medium 
      containing 0.17 M urea, ureolytic microbes with high potential urease activity (> 
      10 mumol urea hydrolyzed per min per ml of enrichment culture) were repeatedly 
      enriched under non-sterile conditions. In addition, the ureolytic enrichment 
      obtained in this study showed in vitro calcium carbonate precipitation. 
      Fluorescence in situ hybridization analysis showed the existence of bacteria of 
      the phylum Firmicutes in the bioreactor system. Our data demonstrate that this 
      DHS bioreactor system is a useful system for the enrichment and production of 
      ureolytic microbes for MICP applications.
FAU - Aoki, Masataka
AU  - Aoki M
AD  - Department of Civil Engineering, National Institute of Technology, Wakayama 
      College,Gobo, Wakayama,Japan.
FAU - Noma, Takuya
AU  - Noma T
AD  - Department of Civil Engineering, National Institute of Technology, Wakayama 
      College,Gobo, Wakayama,Japan.
FAU - Yonemitsu, Hiroshi
AU  - Yonemitsu H
AD  - Department of Applied Chemistry and Biochemistry, National Institute of 
      Technology, Wakayama College,Gobo, Wakayama,Japan.
FAU - Araki, Nobuo
AU  - Araki N
AD  - Department of Civil Engineering, National Institute of Technology, Nagaoka 
      College,Nagaoka, Niigata,Japan.
FAU - Yamaguchi, Takashi
AU  - Yamaguchi T
AD  - Department of Science of Technology Innovation, Nagaoka University of 
      Technology,Nagaoka, Niigata,Japan.
FAU - Hayashi, Kazuyuki
AU  - Hayashi K
AD  - Department of Civil Engineering, National Institute of Technology, Wakayama 
      College,Gobo, Wakayama,Japan.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Pol J Microbiol
JT  - Polish journal of microbiology
JID - 101229003
RN  - 8W8T17847W (Urea)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bioreactors/*microbiology
MH  - Calcium Carbonate
MH  - Chemical Precipitation
MH  - Firmicutes/classification/*growth & development
MH  - In Situ Hybridization, Fluorescence
MH  - Urea/*metabolism
MH  - Urease/analysis
OTO - NOTNLM
OT  - DHS bioreactor system
OT  - enrichment culture
OT  - microbially induced carbonate precipitation (MICP)
OT  - ureolytic microbes
EDAT- 2018/07/18 06:00
MHDA- 2019/06/07 06:00
CRDT- 2018/07/18 06:00
PHST- 2017/09/27 00:00 [accepted]
PHST- 2018/07/18 06:00 [entrez]
PHST- 2018/07/18 06:00 [pubmed]
PHST- 2019/06/07 06:00 [medline]
AID - 10.5604/01.3001.0011.6144 [doi]
PST - ppublish
SO  - Pol J Microbiol. 2018 Mar 9;67(1):59-65. doi: 10.5604/01.3001.0011.6144.