PMID- 30015884
OWN - NLM
STAT- MEDLINE
DCOM- 20181026
LR  - 20181114
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 18
IP  - 3
DP  - 2018 Sep
TI  - Investigation of the role of cullin 4A overexpression in human liver cancer.
PG  - 2531-2540
LID - 10.3892/mmr.2018.9233 [doi]
AB  - Cullin 4A (CUL4A) is the major component of cullin‑RING‑based E3 
      ubiquitin‑protein ligase complexes, which regulate the ubiquitination of target 
      proteins. The overexpression of CUL4A has been associated with the development 
      and progression of various cancer types. However, a detailed understanding of the 
      role of CUL4A in human liver cancer has not been determined by previous studies. 
      In the present study, the association between human liver cancer and CUL4A 
      expression was investigated. The expression of CUL4A in liver cancer tissues and 
      paracancerous tissues of patients was investigated by reverse 
      transcription‑quantitative polymerase chain reaction, western blotting and 
      immunohistochemical staining. Overexpression and knockdown of CUL4A were induced 
      with an overexpression vector and small interfering RNA transfection, 
      respectively, in human liver cancer cell lines, and the effects on cell 
      proliferation were analyzed by a Cell Counting Kit‑8 assay to investigate the 
      role of CUL4A in human liver cancer. Cell migration, invasion, apoptosis and the 
      cell cycle were also analyzed following transfection. The results of the present 
      study revealed that the mRNA and protein expression of CUL4A was increased in the 
      liver cancer tissues compared with the paracancerous tissues of 3 patients. 
      Additionally, the results demonstrated that downregulation of CUL4A expression 
      inhibited cell proliferation, migration and invasion, and increased the 
      percentage of cell apoptosis, in HEPG2 and MHCC97‑H cells, while CUL4A 
      overexpression led to the opposite effects. Therefore, the results of the current 
      study indicated that CUL4A may serve an important role in the development and 
      progression of human liver cancer, and highlights the potential of CUL4A as a 
      novel target in the diagnosis and treatment of human liver cancer and potentially 
      other cancer types.
FAU - Chen, Gang
AU  - Chen G
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Zhao, Xiongqi
AU  - Zhao X
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Tan, Zedan
AU  - Tan Z
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Wang, Dongdong
AU  - Wang D
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Luo, Ding
AU  - Luo D
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Zhang, Peiyao
AU  - Zhang P
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Cao, Jun
AU  - Cao J
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Wang, Fan
AU  - Wang F
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Liu, Qiyu
AU  - Liu Q
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
FAU - Li, Li
AU  - Li L
AD  - Department of Hepatobiliary Surgery, First People's Hospital of Kunming, Kunming, 
      Yunnan 650032, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180629
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (CUL4A protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Claudin-3)
RN  - 0 (Cullin Proteins)
RN  - 0 (Occludin)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Apoptosis
MH  - Cadherins/metabolism
MH  - Cell Cycle Checkpoints
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Claudin-3/metabolism
MH  - Cullin Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver/metabolism/pathology
MH  - Liver Neoplasms/metabolism/*pathology
MH  - Occludin/metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
PMC - PMC6102737
EDAT- 2018/07/18 06:00
MHDA- 2018/10/27 06:00
PMCR- 2018/06/29
CRDT- 2018/07/18 06:00
PHST- 2017/09/15 00:00 [received]
PHST- 2018/01/09 00:00 [accepted]
PHST- 2018/07/18 06:00 [pubmed]
PHST- 2018/10/27 06:00 [medline]
PHST- 2018/07/18 06:00 [entrez]
PHST- 2018/06/29 00:00 [pmc-release]
AID - mmr-18-03-2531 [pii]
AID - 10.3892/mmr.2018.9233 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Sep;18(3):2531-2540. doi: 10.3892/mmr.2018.9233. Epub 2018 Jun 
      29.