PMID- 30015907
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20220121
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 42
IP  - 4
DP  - 2018 Oct
TI  - miR‑330‑5p inhibits H2O2‑induced adipogenic differentiation of MSCs by regulating 
      RXRgamma.
PG  - 2042-2052
LID - 10.3892/ijmm.2018.3773 [doi]
AB  - The elucidation of the underlying molecular mechanism of H2O2‑induced adipocyte 
      differentiation in mesenchymal stem cells (MSCs) is important for the development 
      of treatments for metabolic diseases. The aim of the present study was to 
      identify microRNA (miR)‑330‑5p, which targets retinoid X receptor gamma (RXRgamma) and to 
      determine the function of H2O2‑induced adipogenic differentiation of MSCs. During 
      differentiation of MSCs into adipocytes induced by H2O2, miR‑330‑5p expression 
      was decreased with a concomitant increase in RXRgamma expression. A luciferase assay 
      with RXRgamma 3'‑untranslated region (UTR) reporter plasmid, including the 
      miR‑330‑5p‑binding sequences, identified that the introduction of miR‑330‑5p 
      decreases luciferase activity. However, it did not affect the activity of mutated 
      RXRgamma 3'‑UTR reporter. Enforced expression of miR‑330‑5p significantly inhibited 
      adipocyte differentiation by decreasing RXRgamma mRNA and protein levels. In 
      contrast, inhibition of the endogenous miR‑330‑5p promoted the formation of lipid 
      droplets by rescuing RXRgamma expression. Furthermore, the effects of inhibition of 
      RXRgamma were similar to those of overexpression of miR‑330‑5p on H2O2‑induced 
      adipogenic differentiation from MSCs. miR‑330‑5p inhibits H2O2‑induced adipogenic 
      differentiation of MSCs, and this is dependent on RXRgamma. Taken together, the 
      results of the present study revealed that miR‑330‑5p acts as a critical 
      regulator of RXRgamma, and is able to determinate the fate of MSCs to differentiate 
      into adipocytes. This suggests that miR‑330‑5p and RXRgamma may be target molecules 
      for controlling metabolic diseases.
FAU - Huang, Weiping
AU  - Huang W
AD  - Research Center of Integrative Medicine, School of Basic Medical Science, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. 
      China.
FAU - Li, Ke
AU  - Li K
AD  - Research Center of Integrative Medicine, School of Basic Medical Science, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. 
      China.
FAU - Liu, Aijun
AU  - Liu A
AD  - Research Center of Integrative Medicine, School of Basic Medical Science, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. 
      China.
FAU - Yang, Zeyu
AU  - Yang Z
AD  - Research Center of Integrative Medicine, School of Basic Medical Science, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. 
      China.
FAU - Hu, Chenxia
AU  - Hu C
AD  - School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510006, P.R. China.
FAU - Chen, Dongfeng
AU  - Chen D
AD  - Research Center of Integrative Medicine, School of Basic Medical Science, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. 
      China.
FAU - Wang, Hongqi
AU  - Wang H
AD  - Research Center of Integrative Medicine, School of Basic Medical Science, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. 
      China.
LA  - eng
PT  - Journal Article
DEP - 20180712
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (MIRN330 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Retinoid X Receptor gamma)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Gene Expression Regulation/*drug effects
MH  - Hydrogen Peroxide/*pharmacology
MH  - Mesenchymal Stem Cells/*metabolism
MH  - MicroRNAs/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinoid X Receptor gamma/*biosynthesis
PMC - PMC6108853
EDAT- 2018/07/18 06:00
MHDA- 2018/11/09 06:00
PMCR- 2018/07/12
CRDT- 2018/07/18 06:00
PHST- 2018/02/06 00:00 [received]
PHST- 2018/07/06 00:00 [accepted]
PHST- 2018/07/18 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/07/18 06:00 [entrez]
PHST- 2018/07/12 00:00 [pmc-release]
AID - ijmm-42-04-2042 [pii]
AID - 10.3892/ijmm.2018.3773 [doi]
PST - ppublish
SO  - Int J Mol Med. 2018 Oct;42(4):2042-2052. doi: 10.3892/ijmm.2018.3773. Epub 2018 
      Jul 12.