PMID- 30016639
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR  - 20191001
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 442
IP  - 1
DP  - 2018 Oct 1
TI  - The phenotypic and functional properties of mouse yolk-sac-derived embryonic 
      macrophages.
PG  - 138-154
LID - S0012-1606(18)30461-5 [pii]
LID - 10.1016/j.ydbio.2018.07.009 [doi]
AB  - Macrophages are well characterized as immune cells. However, in recent years, a 
      multitude of non-immune functions have emerged many of which play essential roles 
      in a variety of developmental processes (Wynn et al., 2013; DeFalco et al., 
      2014). In adult animals, macrophages are derived from circulating monocytes 
      originating in the bone marrow, but much of the tissue-resident population arise 
      from erythro-myeloid progenitors (EMPs) in the extra-embryonic yolk sac, 
      appearing around the same time as primitive erythroblasts (Schulz et al., 2012; 
      Kierdorf et al., 2013; McGrath et al., 2015; Gomez Perdiguero et al., 2015; Mass 
      et al., 2016). Of particular interest to our group, macrophages have been shown 
      to act as pro-angiogenic regulators during development (Wynn et al., 2013; 
      DeFalco et al., 2014; Hsu et al., 2015), but there is still much to learn about 
      these early cells. The goal of the present study was to isolate and expand 
      progenitors of yolk-sac-derived Embryonic Macrophages (EMs) in vitro to generate 
      a new platform for mechanistic studies of EM differentiation. To accomplish this 
      goal, we isolated pure (>98%) EGFP(+) populations by flow cytometry from 
      embryonic day 9.5 (E9.5) Csf1r-EGFP(+/tg) mice, then evaluated the angiogenic 
      potential of EMs relative to Bone Marrow-Derived Macrophages (BMDMs). We found 
      that EMs expressed more pro-angiogenic and less pro-inflammatory macrophage 
      markers than BMDMs. EMs also promoted more endothelial cell (EC) cord formation 
      in vitro, as compared to BMDMs in a manner that required direct cell-to-cell 
      contact. Importantly, EMs preferentially matured into microglia when co-cultured 
      with mouse Neural Stem/Progenitor Cells (NSPCs). In conclusion, we have 
      established a protocol to isolate and propagate EMs in vitro, have further 
      defined specialized properties of yolk-sac-derived macrophages, and have 
      identified EM-EC and EM-NSPC interactions as key inducers of EC tube formation 
      and microglial cell maturation, respectively.
CI  - Copyright (c) 2018 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Yosef, Nejla
AU  - Yosef N
AD  - Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 
      Houston, TX, USA.
FAU - Vadakkan, Tegy J
AU  - Vadakkan TJ
AD  - Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 
      Houston, TX, USA.
FAU - Park, June-Hee
AU  - Park JH
AD  - Department of Neurology, Yale University, New Haven, CT, USA.
FAU - Poche, Ross A
AU  - Poche RA
AD  - Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 
      Houston, TX, USA.
FAU - Thomas, Jean-Leon
AU  - Thomas JL
AD  - Department of Neurology, Yale University, New Haven, CT, USA; Sorbonne 
      Universites UPMC Univ Paris 06, Inserm, CNRS, APHP, Institut du Cerveau et de la 
      Moelle epiniere (ICM), GH Pitie-Salpetriere, Paris, France.
FAU - Dickinson, Mary E
AU  - Dickinson ME
AD  - Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 
      Houston, TX, USA; Department of Molecular and Human Genetics, Baylor College of 
      Medicine, Houston, TX, USA; Cardiovascular Research Institute, Baylor College of 
      Medicine, Houston, TX, USA; Department of Bioengineering, Rice University, 
      Houston, TX, USA. Electronic address: mdickins@bcm.edu.
LA  - eng
GR  - R01 EB016629/EB/NIBIB NIH HHS/United States
GR  - R01 HL128064/HL/NHLBI NIH HHS/United States
GR  - P30 CA125123/CA/NCI NIH HHS/United States
GR  - P30 AI036211/AI/NIAID NIH HHS/United States
GR  - S10 RR024574/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180730
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/physiology
MH  - Coculture Techniques/methods
MH  - Erythroid Precursor Cells/*physiology
MH  - Flow Cytometry/methods
MH  - Hematopoietic Stem Cells/physiology
MH  - Macrophages/cytology/*physiology
MH  - Mice/embryology
MH  - Myeloid Progenitor Cells/*physiology
MH  - Phenotype
MH  - Yolk Sac/cytology
PMC - PMC6190604
MID - NIHMS1503299
OTO - NOTNLM
OT  - Angiogenesis
OT  - Endothelial Cells
OT  - Macrophages
OT  - Microglia
OT  - Neural Stem/Progenitor Cells
OT  - Yolk sac
EDAT- 2018/07/18 06:00
MHDA- 2018/10/03 06:00
PMCR- 2019/10/01
CRDT- 2018/07/18 06:00
PHST- 2018/07/04 00:00 [received]
PHST- 2018/07/11 00:00 [accepted]
PHST- 2018/07/18 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/07/18 06:00 [entrez]
PHST- 2019/10/01 00:00 [pmc-release]
AID - S0012-1606(18)30461-5 [pii]
AID - 10.1016/j.ydbio.2018.07.009 [doi]
PST - ppublish
SO  - Dev Biol. 2018 Oct 1;442(1):138-154. doi: 10.1016/j.ydbio.2018.07.009. Epub 2018 
      Jul 30.