PMID- 3001665
OWN - NLM
STAT- MEDLINE
DCOM- 19860219
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 6
IP  - 5
DP  - 1985 Sep-Oct
TI  - An investigation on pro-opiomelanocortin and processed peptides from the teleost 
      fish Prochilodus platensis.
PG  - 817-24
AB  - Acid extracts of carefully dissected proadenohypophysis (PA) and 
      metaadenohypophysis (MA) of the teleost Prochilodus platensis were subjected to 
      chromatography in Sephadex G-50 after which several pro-opiomelanocortin (POMC) 
      peptides were detected by means of three heterologous RIA systems: alpha-MSH, 
      ACTH and beta-endorphin. Parallelism among extracts displacement curves ranged 
      from 26% to 95% of those of the standard curves for the different systems 
      employed. In PA chromatograms, peaks of ACTH immunoreactivity (IR) were detected 
      at the positions of 30 kilodalton (K), 20K, 9K, a large 4.5K peak and 2K. Only 
      one peak of beta-endorphin IR was detected at 30K. In MA chromatograms, ACTH IR 
      detected similar peaks as in PA runs, but 4.5K peak was much smaller, whereas a 
      large 2K peak roughly coincided with all alpha-MSH detected in the chromatograms. 
      beta-Endorphin IR was detected mainly as a large peak coinciding with synthetic 
      beta-endorphin in MA runs. Bioactivity was detected in both PA and MA 4.5K ACTH 
      peaks, whereas little activity could be demonstrated associated with the 30K, 20K 
      and 9K ACTH IR peaks. Prochilodus PAs and MAs were incubated with tritiated 
      aminoacids and the extracts immunoprecipitated with ACTH, beta-endorphin and 
      N-terminal POMC (N-POMC) antisera. The dissociated complexes were run in SDS 
      polyacrylamide slab gel electrophoresis. The tritiated bands detected confirmed 
      the results obtained with Sephadex chromatography. N-POMC immunoprecipitated 
      peptides were located at 28K, 18K and 9K positions. The first two probably 
      accounted for POMC and the N-POMC/ACTH intermediate respectively; the third 
      corresponded to the mammalian 1-76N-POMC.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Estivariz, F E
AU  - Estivariz FE
FAU - Iturriza, F C
AU  - Iturriza FC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Endorphins)
RN  - 60617-12-1 (beta-Endorphin)
RN  - 66796-54-1 (Pro-Opiomelanocortin)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - 9002-79-3 (Melanocyte-Stimulating Hormones)
RN  - 9035-55-6 (beta-Lipotropin)
SB  - IM
MH  - Adrenocorticotropic Hormone/analysis
MH  - Animals
MH  - Endorphins/analysis
MH  - Fishes/*metabolism
MH  - Melanocyte-Stimulating Hormones/analysis
MH  - Pituitary Gland/analysis
MH  - Pro-Opiomelanocortin/*analysis
MH  - Protein Processing, Post-Translational
MH  - Radioimmunoassay
MH  - beta-Endorphin
MH  - beta-Lipotropin/analysis
EDAT- 1985/09/01 00:00
MHDA- 1985/09/01 00:01
CRDT- 1985/09/01 00:00
PHST- 1985/09/01 00:00 [pubmed]
PHST- 1985/09/01 00:01 [medline]
PHST- 1985/09/01 00:00 [entrez]
AID - 0196-9781(85)90307-9 [pii]
AID - 10.1016/0196-9781(85)90307-9 [doi]
PST - ppublish
SO  - Peptides. 1985 Sep-Oct;6(5):817-24. doi: 10.1016/0196-9781(85)90307-9.