PMID- 30019620
OWN - NLM
STAT- MEDLINE
DCOM- 20191101
LR  - 20200930
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 17
IP  - 10
DP  - 2018
TI  - An engineered novel lentivector specifically transducing dendritic cells and 
      eliciting robust HBV-specific CTL response by upregulating autophagy in T cells.
PG  - 1220-1234
LID - 10.1080/15384101.2018.1471312 [doi]
AB  - Dendritic cells (DCs) play a predominant role in initiating cell immune 
      responses. Here we generated a DC-targeting lentiviral vector 
      (LVDC-UbHBcAg-LIGHT) and evaluated its capacity to elicit HBV-specific cytotoxic 
      T lymphocyte (CTL) responses. DC-SIGN-mediated specific transduction using this 
      construct was confirmed in DC-SIGN-expressing 293T cells and ex vivo-cultured 
      bone marrow cells. LVDC-UbHBcAg-LIGHT-loaded DCs were highly effective in 
      inducing HBV-specific CTLs. Mechanistic studies demonstrated autophagy blocking 
      led to a significant increase in apoptosis and obvious inhibition of CD8 + T 
      cells entry into S-phase, correspondingly attenuated LVDC-UbHBcAg-LIGHT-loaded 
      DC-induced T cell responses. This observation was supported by accumulation of 
      pro-apoptotic proteins and the main negative cell cycle regulator-CDKN1B that 
      otherwise would be degraded in activated T cells where autophagy preferentially 
      occured. Our findings revealed an important role of autophagy in the activation 
      of T cells and suggested LVDC-UbHBcAg-LIGHT may potentially be used as a 
      therapeutic strategy to combat persistent HBV infection with higher security.
FAU - Ma, Siyuan
AU  - Ma S
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Chen, Xiaohua
AU  - Chen X
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Tan, Quanhui
AU  - Tan Q
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Li, Dan
AU  - Li D
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Dai, Shenglan
AU  - Dai S
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Wu, Shanshan
AU  - Wu S
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Yu, Yongsheng
AU  - Yu Y
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Zang, Guoqing
AU  - Zang G
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Tang, Zhenghao
AU  - Tang Z
AD  - a Department of Infectious Disease , Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180718
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Autophagy-Related Protein 5)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Hepatitis B Core Antigens)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Necrosis Factor Ligand Superfamily Member 14)
RN  - 0 (Ubiquitin)
RN  - 5142-23-4 (3-methyladenine)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - Adenine/analogs & derivatives/pharmacology
MH  - Animals
MH  - Apoptosis/drug effects
MH  - *Autophagy/drug effects
MH  - Autophagy-Related Protein 5/metabolism
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Adhesion Molecules/metabolism
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Genetic Engineering
MH  - Genetic Vectors/*metabolism
MH  - Hepatitis B Core Antigens/metabolism
MH  - Hepatitis B virus/*immunology
MH  - Humans
MH  - Lectins, C-Type/metabolism
MH  - Lentivirus/*genetics
MH  - Lymphocyte Activation/drug effects/immunology
MH  - Mice, Inbred BALB C
MH  - Receptors, Cell Surface/metabolism
MH  - S Phase/drug effects
MH  - T-Lymphocytes, Cytotoxic/cytology/drug effects/*immunology/ultrastructure
MH  - *Transduction, Genetic
MH  - Tumor Necrosis Factor Ligand Superfamily Member 14/metabolism
MH  - Ubiquitin/metabolism
MH  - *Up-Regulation/drug effects
PMC - PMC6110602
OTO - NOTNLM
OT  - Dendritic cell (DC)-targeting lentivector
OT  - autophagy
OT  - cytotoxic T lymphocyte (CTL)
EDAT- 2018/07/19 06:00
MHDA- 2019/11/02 06:00
PMCR- 2019/07/18
CRDT- 2018/07/19 06:00
PHST- 2018/07/19 06:00 [pubmed]
PHST- 2019/11/02 06:00 [medline]
PHST- 2018/07/19 06:00 [entrez]
PHST- 2019/07/18 00:00 [pmc-release]
AID - 1471312 [pii]
AID - 10.1080/15384101.2018.1471312 [doi]
PST - ppublish
SO  - Cell Cycle. 2018;17(10):1220-1234. doi: 10.1080/15384101.2018.1471312. Epub 2018 
      Jul 18.