PMID- 30021208
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 177
IP  - 3
DP  - 2018
TI  - Degradation of Monocyte Chemoattractant Protein-1 by Tryptase Co-Released in 
      Immunoglobulin E-Dependent Activation of Primary Human Cultured Mast Cells.
PG  - 199-206
LID - 10.1159/000490533 [doi]
AB  - BACKGROUND: Mast cells are key immune effector cells which release chemokines, 
      proteases, and other inflammatory mediators upon activation by immunological 
      stimuli. The aim of this study was to investigate the effects of co-releasing 
      proteases on the kinetics of release of the chemokine monocyte chemoattractant 
      protein-1 (MCP-1) in immunoglobulin E (IgE)-mediated activation of human mast 
      cells. METHODS: Homogenous populations of mature and functional primary human 
      mast cells were generated from CD34+ progenitors originated from buffy coats of 
      healthy adult donors. The releases of MCP-1 from human mast cells in basal 
      conditions and in response to FcepsilonRI cross-linking were assessed at different time 
      points. The effects of different types of protease inhibitors on MCP-1 release 
      from these mast cells under stimulated or unstimulated conditions were also 
      investigated. RESULTS: Cultured human mast cells released MCP-1 in basal 
      conditions and its levels increased in a time-dependent manner. When stimulated 
      by FcepsilonRI cross-linking, the levels of MCP-1 detected in the medium gradually 
      decreased over time after the initial peak induction. Such a decline in MCP-1 
      levels after IgE-dependent activation was completely prevented by pretreatment 
      with a cocktail of protease inhibitors or the specific tryptase inhibitor APC366. 
      CONCLUSIONS: Direct regulation of MCP-1 expression by co-release of tryptase in 
      cultured human mast cells upon IgE-dependent activation demonstrates a role of 
      the serglycin:serine protease axis in modulation of inflammatory reactions 
      through proteolytic degradation of mediators such as chemokines.
CI  - (c) 2018 S. Karger AG, Basel.
FAU - Tam, Issan Yee San
AU  - Tam IYS
AD  - School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong 
      Kong, Shatin, China.
FAU - Ng, Chun Wai
AU  - Ng CW
AD  - School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong 
      Kong, Shatin, China.
FAU - Lau, Hang Yung Alaster
AU  - Lau HYA
AD  - School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong 
      Kong, Shatin, China.
FAU - Tam, See-Ying
AU  - Tam SY
AD  - Department of Pathology, Stanford University School of Medicine, Stanford, 
      California, USA.
AD  - Sean N. Parker Center for Allergy and Asthma Research, Stanford University School 
      of Medicine, Stanford, California, USA.
LA  - eng
PT  - Journal Article
DEP - 20180718
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Proteoglycans)
RN  - 0 (Receptors, IgE)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (serglycin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.- (Serine Proteases)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Cell Degranulation/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Histamine Release/immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Mast Cells/*immunology/physiology
MH  - Protease Inhibitors/pharmacology
MH  - Proteoglycans/*metabolism
MH  - Receptors, IgE/immunology/metabolism
MH  - Serine Proteases/*metabolism
MH  - Tryptases/antagonists & inhibitors/*metabolism
MH  - Vesicular Transport Proteins/*metabolism
OTO - NOTNLM
OT  - Human mast cells
OT  - Immunoglobulin E
OT  - Mast cell tryptase
OT  - Mediator release
OT  - Monocyte chemoattractant protein-1
EDAT- 2018/07/19 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/07/19 06:00
PHST- 2018/04/12 00:00 [received]
PHST- 2018/05/29 00:00 [accepted]
PHST- 2018/07/19 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/07/19 06:00 [entrez]
AID - 000490533 [pii]
AID - 10.1159/000490533 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2018;177(3):199-206. doi: 10.1159/000490533. Epub 2018 
      Jul 18.