PMID- 30022471
OWN - NLM
STAT- MEDLINE
DCOM- 20190812
LR  - 20191001
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 194
IP  - 1
DP  - 2018 Oct
TI  - Functional status of immune cells in patients with long-lasting type 2 diabetes 
      mellitus.
PG  - 125-136
LID - 10.1111/cei.13187 [doi]
AB  - Although patients with diabetes contract infectious diseases at higher 
      frequencies, and in more severe forms, compared to non-diabetics, the underlying 
      defects of the immune function have not been defined clearly. To address this, we 
      designed an immune monitoring protocol and analysed the functional status of 
      various immune cells. Peripheral blood mononuclear cells (PBMCs) were stimulated 
      with the proper ligands and the functional reactivity of each lineage of cells 
      was subsequently measured. Patients with type 2 diabetes mellitus (T2DM) had PBMC 
      composition ratios comparable to healthy controls, except for a higher frequency 
      of B cell and effector T cell fractions. The capacity of myeloid cells to secrete 
      proinflammatory cytokines was not diminished in terms of the sensitivity and 
      magnitude of the response. Furthermore, cytolytic activity and interferon (IFN)-gamma 
      production of natural killer (NK) cells and CD8(+) T cells were not decreased in 
      T2DM patients. Phenotypical maturation of dendritic cells, indicated by the 
      up-regulation of major histocompatibility complex (MHC) proteins and 
      co-stimulatory molecules in response to lipopolysaccharide (LPS), was slightly 
      enhanced in T2DM patients. Finally, the functional differentiation profiles of 
      CD4(+) T cells did not differ between T2DM patients and the control group. These 
      data indicate that patients with long-lasting T2DM do not have any gross 
      functional defects in immune cells, at least in circulating monocytes, dendritic 
      cells, NK cells and T lymphocytes.
CI  - (c) 2018 British Society for Immunology.
FAU - Nam, H W
AU  - Nam HW
AD  - Division of Endocrinology, Department of Internal Medicine, Seoul, Korea.
FAU - Cho, Y J
AU  - Cho YJ
AD  - Division of Endocrinology, Department of Internal Medicine, Seoul, Korea.
FAU - Lim, J A
AU  - Lim JA
AD  - Division of Endocrinology, Department of Internal Medicine, Seoul, Korea.
FAU - Kim, S J
AU  - Kim SJ
AD  - Department of Family Medicine, National Medical Center, Seoul, Korea.
FAU - Kim, H
AU  - Kim H
AD  - Center for Chronic Diseases, Seoul, Korea.
FAU - Sim, S Y
AU  - Sim SY
AD  - Research Institute, National Medical Center, Seoul, Korea.
FAU - Lim, D G
AU  - Lim DG
AD  - Center for Chronic Diseases, Seoul, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180912
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Cytokines)
RN  - 0 (IFNG protein, human)
RN  - 82115-62-6 (Interferon-gamma)
MH  - Aged
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/cytology/*immunology
MH  - Diabetes Mellitus, Type 2/*immunology
MH  - Female
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Killer Cells, Natural/*immunology
MH  - Leukocytes, Mononuclear/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
PMC - PMC6156808
OTO - NOTNLM
OT  - immune cells
OT  - immune function
OT  - immune monitoring
OT  - type 2 diabetes mellitus
EDAT- 2018/07/20 06:00
MHDA- 2019/08/14 06:00
PMCR- 2019/10/01
CRDT- 2018/07/20 06:00
PHST- 2018/07/11 00:00 [accepted]
PHST- 2018/07/20 06:00 [pubmed]
PHST- 2019/08/14 06:00 [medline]
PHST- 2018/07/20 06:00 [entrez]
PHST- 2019/10/01 00:00 [pmc-release]
AID - CEI13187 [pii]
AID - 10.1111/cei.13187 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2018 Oct;194(1):125-136. doi: 10.1111/cei.13187. Epub 2018 Sep 
      12.