PMID- 30023361
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 5
DP  - 2018
TI  - Pooled Platelet-Rich Plasma Lysate Therapy Increases Synoviocyte Proliferation 
      and Hyaluronic Acid Production While Protecting Chondrocytes From 
      Synoviocyte-Derived Inflammatory Mediators.
PG  - 150
LID - 10.3389/fvets.2018.00150 [doi]
LID - 150
AB  - Platelet-rich plasma (PRP) preparations are being used with moderate success to 
      treat osteoarthritis (OA) in humans and in veterinary species. Such preparations 
      are hindered, however, by being autologous in nature and subject to tremendous 
      patient and processing variability. For this reason, there has been increasing 
      interest in the use of platelet lysate preparations instead of traditional PRP. 
      Platelet lysate preparations are acellular, thereby reducing concerns over 
      immunogenicity, and contain high concentrations of growth factors and cytokines. 
      In addition, platelet lysate preparations can be stored frozen for readily 
      available use. The purpose of this study was to evaluate the effects of a pooled 
      allogeneic platelet-rich plasma lysate (PRP-L) preparation on equine synoviocytes 
      and chondrocytes challenged with inflammatory mediators in-vitro to mimic the OA 
      joint environment. Our hypothesis was that PRP-L treatment of inflamed 
      synoviocytes would protect chondrocytes challenged with synoviocyte conditioned 
      media by reducing synoviocyte pro-inflammatory cytokine production while 
      increasing synoviocyte anti-inflammatory cytokine production. Synoviocytes were 
      stimulated with either interleukin-1beta (IL-1beta) or lipopolysaccharide (LPS) for 24 
      h followed by no treatment or treatment with platelet-poor plasma lysate (PPP-L) 
      or PRP-L for 48 h. Synoviocyte growth was evaluated at the end of the treatment 
      period and synoviocyte conditioned media was assessed for concentrations of 
      hyaluronic acid (HA), IL-1beta, tumor necrosis factor alpha (TNF-alpha), and 
      interleukin-6 (IL-6). Chondrocytes were then challenged for 48 h with synoviocyte 
      conditioned media from each stimulation and treatment group and examined for gene 
      expression of collagen types I (COL1A1), II (COL2A1), and III (COL3A1), aggrecan 
      (ACAN), lubricin (PRG4), and matrix metallopeptidase 3 (MMP-3) and 13 (MMP-13). 
      Treatment of inflamed synoviocytes with PRP-L resulted in increased synoviocyte 
      growth and increased synoviocyte HA and IL-6 production. Challenge of 
      chondrocytes with conditioned media from PRP-L treated synoviocytes resulted in 
      increased collagen type II and aggrecan gene expression as well as decreased 
      MMP-13 gene expression. The results of this study support continued investigation 
      into the use of pooled PRP-L for the treatment of osteoarthritis and warrant 
      further in-vitro studies to discern the mechanisms of action of PRP-L.
FAU - Gilbertie, Jessica M
AU  - Gilbertie JM
AD  - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina 
      State University, Raleigh, NC, United States.
AD  - Comparative Medicine Institute, North Carolina State University, Raleigh, NC, 
      Unites States.
FAU - Long, Julie M
AU  - Long JM
AD  - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina 
      State University, Raleigh, NC, United States.
FAU - Schubert, Alicia G
AU  - Schubert AG
AD  - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina 
      State University, Raleigh, NC, United States.
FAU - Berglund, Alix K
AU  - Berglund AK
AD  - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina 
      State University, Raleigh, NC, United States.
AD  - Comparative Medicine Institute, North Carolina State University, Raleigh, NC, 
      Unites States.
FAU - Schaer, Thomas P
AU  - Schaer TP
AD  - Department of Clinical Studies New Bolton Center, School of Veterinary Medicine, 
      University of Pennsylvania, Kennett Square, PA, United States.
FAU - Schnabel, Lauren V
AU  - Schnabel LV
AD  - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina 
      State University, Raleigh, NC, United States.
AD  - Comparative Medicine Institute, North Carolina State University, Raleigh, NC, 
      Unites States.
LA  - eng
PT  - Journal Article
DEP - 20180704
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC6039577
OTO - NOTNLM
OT  - HA
OT  - IL-1beta
OT  - LPS
OT  - MMP-13
OT  - aggrecan
OT  - collagen type II
OT  - osteoarthritis
OT  - platelet-rich plasma lysate
EDAT- 2018/07/20 06:00
MHDA- 2018/07/20 06:01
PMCR- 2018/01/01
CRDT- 2018/07/20 06:00
PHST- 2018/03/16 00:00 [received]
PHST- 2018/06/15 00:00 [accepted]
PHST- 2018/07/20 06:00 [entrez]
PHST- 2018/07/20 06:00 [pubmed]
PHST- 2018/07/20 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2018.00150 [doi]
PST - epublish
SO  - Front Vet Sci. 2018 Jul 4;5:150. doi: 10.3389/fvets.2018.00150. eCollection 2018.