PMID- 30027430
OWN - NLM
STAT- MEDLINE
DCOM- 20190312
LR  - 20240407
IS  - 1878-7479 (Electronic)
IS  - 1933-7213 (Print)
IS  - 1878-7479 (Linking)
VI  - 15
IP  - 4
DP  - 2018 Oct
TI  - A Dendritic Cell-Targeted Adenoviral Vector Facilitates Adaptive Immune Response 
      Against Human Glioma Antigen (CMV-IE) and Prolongs Survival in a Human Glioma 
      Tumor Model.
PG  - 1127-1138
LID - 10.1007/s13311-018-0650-3 [doi]
AB  - Antitumor immunotherapeutic strategies represent an especially promising set of 
      approaches with rapid translational potential considering the dismal clinical 
      context of high-grade gliomas. Dendritic cells (DCs) are the body's most 
      professional antigen-presenting cells, able to recruit and activate T cells to 
      stimulate an adaptive immune response. In this regard, specific loading of 
      tumor-specific antigen onto dendritic cells potentially represents one of the 
      most advanced strategies to achieve effective antitumor immunization. In this 
      study, we developed a DC-specific adenoviral (Ad) vector, named Ad5scFvDEC205FF, 
      targeting the DC surface receptor, DEC205. In vitro analysis shows that 60% of 
      DCs was infected by this vector while the infectivity of other control adenoviral 
      vectors was less than 10%, demonstrating superior infectivity on DCs. Moreover, 
      an average of 14% of DCs were infected by Ad5scFvDEC205FF-GFP, while less than 3% 
      of non-DCs were infected following in vivo administration, demonstrating highly 
      selective in vivo DC infection. Importantly, vaccination with this vehicle 
      expressing human glioma-specific antigen, Ad5scFvDEC205FF-CMV-IE, shows a 
      prolonged survival benefit in GL261(CMV-IE)-implanted murine glioma models 
      (p < 0.0007). Furthermore, when rechallenged, cancerous cells were completely 
      rejected. In conclusion, our novel, viral-mediated, DC-based immunization 
      approach has the significant therapeutic potential for patients with high-grade 
      gliomas.
FAU - Kim, Julius W
AU  - Kim JW
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Kane, J Robert
AU  - Kane JR
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Panek, Wojciech K
AU  - Panek WK
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Young, Jacob S
AU  - Young JS
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Rashidi, Aida
AU  - Rashidi A
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Yu, Dou
AU  - Yu D
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Kanojia, Deepak
AU  - Kanojia D
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Hasan, Tanwir
AU  - Hasan T
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Miska, Jason
AU  - Miska J
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Gomez-Lim, Miguel A
AU  - Gomez-Lim MA
AD  - Departamento de Ingenieria Genetica, CINVESTAV Irapuato, Km 9.6 Libramiento Norte 
      Carretera Irapuato-Leon, 36821, Irapuato, Guanajuato, Mexico.
FAU - Ulasov, Ilya V
AU  - Ulasov IV
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Balyasnikova, Irina V
AU  - Balyasnikova IV
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Ahmed, Atique U
AU  - Ahmed AU
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Wainwright, Derek A
AU  - Wainwright DA
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA.
FAU - Lesniak, Maciej S
AU  - Lesniak MS
AD  - Department of Neurological Surgery, Northwestern University, 676 North Saint 
      Clair Street, Suite 2210, Chicago, Illinois, 60611, USA. 
      maciej.lesniak@northwestern.edu.
LA  - eng
GR  - F32 NS073366/NS/NINDS NIH HHS/United States
GR  - R00 NS082381/NS/NINDS NIH HHS/United States
GR  - R01 NS093903/NS/NINDS NIH HHS/United States
GR  - R35CA197725/NH/NIH HHS/United States
GR  - R01NS093903/NH/NIH HHS/United States
GR  - K99 NS082381/NS/NINDS NIH HHS/United States
GR  - R01CA122930/NH/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurotherapeutics
JT  - Neurotherapeutics : the journal of the American Society for Experimental 
      NeuroTherapeutics
JID - 101290381
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Adaptive Immunity/*genetics
MH  - Adenoviridae/genetics
MH  - Analysis of Variance
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Antigens, Neoplasm/*metabolism
MH  - *Brain Neoplasms/immunology/pathology/therapy
MH  - Dendritic Cells/*immunology/virology
MH  - Disease Models, Animal
MH  - Genetic Vectors/metabolism
MH  - *Glioma/immunology/pathology/therapy
MH  - HEK293 Cells
MH  - Humans
MH  - Lectins, C-Type/*metabolism
MH  - Lymph Nodes/cytology
MH  - Mice
MH  - Minor Histocompatibility Antigens/*metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Spleen/cytology
MH  - Transduction, Genetic
MH  - Transfection
MH  - Xenograft Model Antitumor Assays
PMC - PMC6277295
OTO - NOTNLM
OT  - adenovirus
OT  - cytomegalovirus
OT  - dendritic cells
OT  - glioblastoma
OT  - vaccine
EDAT- 2018/07/22 06:00
MHDA- 2019/03/13 06:00
PMCR- 2019/10/01
CRDT- 2018/07/21 06:00
PHST- 2018/07/22 06:00 [pubmed]
PHST- 2019/03/13 06:00 [medline]
PHST- 2018/07/21 06:00 [entrez]
PHST- 2019/10/01 00:00 [pmc-release]
AID - S1878-7479(23)01037-1 [pii]
AID - 650 [pii]
AID - 10.1007/s13311-018-0650-3 [doi]
PST - ppublish
SO  - Neurotherapeutics. 2018 Oct;15(4):1127-1138. doi: 10.1007/s13311-018-0650-3.