PMID- 30029405
OWN - NLM
STAT- MEDLINE
DCOM- 20181018
LR  - 20181018
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 188
DP  - 2018 Oct 1
TI  - Disposable electrochemical immunosensor for cortisol determination in human 
      saliva.
PG  - 50-57
LID - S0039-9140(18)30507-1 [pii]
LID - 10.1016/j.talanta.2018.05.039 [doi]
AB  - Cortisol is a steroidal hormone and an important stress marker. Free serum 
      cortisol concentration has been identified to correlate well with free salivary 
      cortisol. In this present work an electrochemical immunosensor was developed to 
      determine cortisol concentration within the physiological concentration range 
      found in human saliva. The immunosensor is based on a direct competitive enzyme 
      linked immunoassay using a home-made cortisol-alkaline phosphatase (AP) conjugate 
      synthesized in our laboratory with disposable graphite screen-printed electrodes 
      (SPEs). 1-nalphtyl phosphate (1-NP) was used as an enzymatic substrate and a 
      square wave voltammetry (SWV) for electrochemical detection. To study method 
      suitability for use with saliva samples, calibration curves were performed both 
      in buffer and saliva. In buffer standard samples showed a limit of detection 
      (LOD) of 0.6 ng/ml and working range (WR) of 0.2-44.6 ng/ml with good 
      reproducibility (RSD 10%). Saliva matrix effect was removed effectively with 
      Salivette Cortisol collection device (polyethylene) and a calibration curve 
      showed similar characteristics as in buffer with LOD 1.7 ng/ml and WR 
      0.5-55.1 ng/ml (RSD 8%) demonstrating the possibility to determine human salivary 
      cortisol within the desired human physiological range. Spiked saliva samples were 
      analyzed with the developed immunosensor presenting excellent 92-114% recovery. 
      Comparison to liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method 
      showed strong 0.90 correlation between methods indicating good accuracy of the 
      developed immunosensor.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Kamarainen, Satu
AU  - Kamarainen S
AD  - University of Oulu, Unit of Measurement Technology (MITY), Kajaani University 
      Consortium, Kehraamontie 7, 87400 Kajaani, Finland; Dipartimento di Scienze e 
      Tecnologie Chimiche, Universita di Roma "Tor Vergata", Via della Ricerca 
      Scientifica, 00133 Roma, Italy.
FAU - Maki, Marianne
AU  - Maki M
AD  - University of Oulu, Unit of Measurement Technology (MITY), Kajaani University 
      Consortium, Kehraamontie 7, 87400 Kajaani, Finland; Dipartimento di Scienze e 
      Tecnologie Chimiche, Universita di Roma "Tor Vergata", Via della Ricerca 
      Scientifica, 00133 Roma, Italy.
FAU - Tolonen, Tiina
AU  - Tolonen T
AD  - University of Oulu, Unit of Measurement Technology (MITY), Kajaani University 
      Consortium, Kehraamontie 7, 87400 Kajaani, Finland; Dipartimento di Scienze e 
      Tecnologie Chimiche, Universita di Roma "Tor Vergata", Via della Ricerca 
      Scientifica, 00133 Roma, Italy.
FAU - Palleschi, Giuseppe
AU  - Palleschi G
AD  - University of Oulu, Unit of Measurement Technology (MITY), Kajaani University 
      Consortium, Kehraamontie 7, 87400 Kajaani, Finland; Dipartimento di Scienze e 
      Tecnologie Chimiche, Universita di Roma "Tor Vergata", Via della Ricerca 
      Scientifica, 00133 Roma, Italy.
FAU - Virtanen, Vesa
AU  - Virtanen V
AD  - University of Oulu, Unit of Measurement Technology (MITY), Kajaani University 
      Consortium, Kehraamontie 7, 87400 Kajaani, Finland; Dipartimento di Scienze e 
      Tecnologie Chimiche, Universita di Roma "Tor Vergata", Via della Ricerca 
      Scientifica, 00133 Roma, Italy.
FAU - Micheli, Laura
AU  - Micheli L
AD  - University of Oulu, Unit of Measurement Technology (MITY), Kajaani University 
      Consortium, Kehraamontie 7, 87400 Kajaani, Finland; Dipartimento di Scienze e 
      Tecnologie Chimiche, Universita di Roma "Tor Vergata", Via della Ricerca 
      Scientifica, 00133 Roma, Italy.
FAU - Sesay, Adama Marie
AU  - Sesay AM
AD  - University of Oulu, Unit of Measurement Technology (MITY), Kajaani University 
      Consortium, Kehraamontie 7, 87400 Kajaani, Finland; Dipartimento di Scienze e 
      Tecnologie Chimiche, Universita di Roma "Tor Vergata", Via della Ricerca 
      Scientifica, 00133 Roma, Italy.
LA  - eng
PT  - Journal Article
DEP - 20180516
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Naphthalenes)
RN  - 0 (Naphthols)
RN  - 0 (Organophosphorus Compounds)
RN  - 2A71EAQ389 (1-naphthol)
RN  - 33881-88-8 (naphthyl phosphate)
RN  - 7440-44-0 (Carbon)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Alkaline Phosphatase/chemistry
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Carbon/chemistry
MH  - Cattle
MH  - Electrochemical Techniques/*methods
MH  - Electrodes
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Goats
MH  - Humans
MH  - Hydrocortisone/*analysis/chemistry/immunology
MH  - Limit of Detection
MH  - Mice
MH  - Naphthalenes/chemistry
MH  - Naphthols/chemistry
MH  - Organophosphorus Compounds/chemistry
MH  - Reproducibility of Results
MH  - Saliva/*chemistry
OTO - NOTNLM
OT  - Cortisol
OT  - Immunoassay
OT  - Matrix effect
OT  - Saliva
OT  - Saliva sampling
OT  - Screen-printed electrode
EDAT- 2018/07/22 06:00
MHDA- 2018/10/20 06:00
CRDT- 2018/07/22 06:00
PHST- 2017/11/27 00:00 [received]
PHST- 2018/05/07 00:00 [revised]
PHST- 2018/05/09 00:00 [accepted]
PHST- 2018/07/22 06:00 [entrez]
PHST- 2018/07/22 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
AID - S0039-9140(18)30507-1 [pii]
AID - 10.1016/j.talanta.2018.05.039 [doi]
PST - ppublish
SO  - Talanta. 2018 Oct 1;188:50-57. doi: 10.1016/j.talanta.2018.05.039. Epub 2018 May 
      16.