PMID- 30029434
OWN - NLM
STAT- MEDLINE
DCOM- 20181018
LR  - 20181018
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 188
DP  - 2018 Oct 1
TI  - Fluorescent reversible regulation based on photoinduced electron transfer from 
      DNA to quantum dots and intercalation binding of DNA intercalator to DNA.
PG  - 7-16
LID - S0039-9140(18)30500-9 [pii]
LID - 10.1016/j.talanta.2018.05.034 [doi]
AB  - Based on the fluorescent reversible regulation, a novel sensor platform was 
      designed for the detection of DNA intercalators utilizing the intercalation 
      binding of DNA intercalators to DNA as an inherent exhibition and the 
      fluorescence change of quantum dots (QDs) as an external manifestation. To prove 
      its feasibility, acridine orange (AO) was chosen as an example of DNA 
      intercalator. When different concentrations of herring sperm DNA (hsDNA) were 
      added to cysteamine (CA)-capped ZnSe QDs solution, the hsDNA bound with the QDs 
      through electrostatic interaction due to the photoinduced electron transfer from 
      hsDNA to QDs and formed QDs-hsDNA complexes with 1:1 ratio, leading to the 
      fluorescence quenching of the QDs; and upon addition of different concentrations 
      of AO to the QDs-hsDNA complex system, the AO first caused the release of the 
      hsDNA from the complexes and concomitantly bound with them through intercalation 
      binding and formed AO-hsDNA complexes with 1:3 ratio on account of the fact that 
      the intercalation binding constant between AO and hsDNA (1.932 x 10(5) L/mol) was 
      greater than the electrostatic interaction constant between QDs and hsDNA 
      (7.874 x 10(4) L/mol), resulting in the fluorescence recovery of the QDs. 
      Therefore, the detection of AO could be achieved through the relationship between 
      the fluorescence recovery yield of the QDs and the concentration of AO added. The 
      results illustrated that the fluorescence recovery yield of the QDs-hsDNA system 
      was linearly dependent to the concentration of AO in the range of 
      5.0-75.0 x 10(-5) mol/L with a detection limit (3sigma/K) of 1.5 x 10(-5) mol/L. This 
      dual-directional fluorescent regulation provided a novel method for the detection 
      of DNA intercalators such as polycyclic aromatic hydrocarbons and drugs 
      interfering with DNA-synthesis and possessed some potential applications in the 
      investigation of the interactions between DNA intercalators and DNA.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Zhang, Yeli
AU  - Zhang Y
AD  - College of Life Science, Northwest University, Xi'an 710069, People's Republic of 
      China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - College of Life Science, Northwest University, Xi'an 710069, People's Republic of 
      China.
FAU - Yang, Wenhui
AU  - Yang W
AD  - College of Life Science, Northwest University, Xi'an 710069, People's Republic of 
      China.
FAU - Bian, Liujiao
AU  - Bian L
AD  - College of Life Science, Northwest University, Xi'an 710069, People's Republic of 
      China. Electronic address: bianliujiao@sohu.com.
LA  - eng
PT  - Journal Article
DEP - 20180509
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Intercalating Agents)
RN  - 5UX2SD1KE2 (Cysteamine)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
RN  - H6241UJ22B (Selenium)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Acridine Orange/*analysis
MH  - Animals
MH  - Cysteamine/chemistry
MH  - DNA/*chemistry/radiation effects
MH  - *Electrons
MH  - Fishes/genetics
MH  - Fluorescence
MH  - Intercalating Agents/*analysis
MH  - Light
MH  - Limit of Detection
MH  - Male
MH  - Quantum Dots/*chemistry
MH  - Selenium/chemistry
MH  - Spectrometry, Fluorescence/*methods
MH  - Spermatozoa/chemistry
MH  - Zinc/chemistry
OTO - NOTNLM
OT  - DNA
OT  - DNA intercalator
OT  - Fluorescence
OT  - Quantum dots
OT  - Reversible regulation
EDAT- 2018/07/22 06:00
MHDA- 2018/10/20 06:00
CRDT- 2018/07/22 06:00
PHST- 2017/11/15 00:00 [received]
PHST- 2018/05/02 00:00 [revised]
PHST- 2018/05/08 00:00 [accepted]
PHST- 2018/07/22 06:00 [entrez]
PHST- 2018/07/22 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
AID - S0039-9140(18)30500-9 [pii]
AID - 10.1016/j.talanta.2018.05.034 [doi]
PST - ppublish
SO  - Talanta. 2018 Oct 1;188:7-16. doi: 10.1016/j.talanta.2018.05.034. Epub 2018 May 
      9.