PMID- 30030770 OWN - NLM STAT- MEDLINE DCOM- 20181121 LR - 20181121 IS - 1573-6903 (Electronic) IS - 0364-3190 (Linking) VI - 43 IP - 9 DP - 2018 Sep TI - Temporal and Site-Specific Changes in Central Neuroimmune Factors During Rapid Weight Gain After Ovariectomy in Rats. PG - 1802-1813 LID - 10.1007/s11064-018-2596-6 [doi] AB - Systemic inflammation is present in obesity and emerging evidence, primarily from studies using male rodents fed high-fat diets, suggests neuroimmune signaling also is involved. We investigated early changes in neuroimmune signaling during the weight gain that follows ovariectomy in rats. Ovariectomized (OVX) rats were given standard rat chow and terminated 5 days (baseline), 4 or 8 weeks after ovariectomy. Levels of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) in plasma and periuterine adipose were not affected by ovariectomy. In contrast, compared to baseline levels, IL-6 expression in the arcuate nucleus (ARC) and dorsal vagal complex (DVC) decreased by 4 weeks after OVX, but was not affected in the paraventricular nucleus (PVN). MCP-1 expression decreased by 4 weeks in the ARC and by 8 weeks in the PVN, but was not affected in the DVC. Increased glial fibrillary acidic protein (GFAP) expression in the PVN indicated astrocyte activation; decreased toll-like receptor 4 (TLR4) expression in the ARC, but not other regions, suggested early effects on innate immune factors. Importantly, in reproductively intact rats, IL-6 and MCP-1 levels in plasma, periuterine adipose, and brain regions were not affected after 8 weeks. Unlike OVX rats, GFAP expression in the DVC of intact rats was decreased at 8 weeks, and TLR4 expression in the ARC was increased at 8 weeks. Taken together, these dynamic and selective changes in neuroimmune factors co-incident with post-ovariectomy weight gain provide insight into the role of neuroimmune signaling in obesity, particularly in females. FAU - Curtis, Kathleen S AU - Curtis KS AD - Oklahoma State University-Center for Health Sciences, Tulsa, OK, 74107, USA. FAU - McCracken, Kelly AU - McCracken K AD - Oklahoma State University-Center for Health Sciences, Tulsa, OK, 74107, USA. FAU - Espinosa, Enith AU - Espinosa E AD - Oklahoma State University-Center for Health Sciences, Tulsa, OK, 74107, USA. FAU - Ong, Johnson AU - Ong J AD - Oklahoma State University-Center for Health Sciences, Tulsa, OK, 74107, USA. FAU - Buck, Daniel J AU - Buck DJ AD - Oklahoma State University-Center for Health Sciences, Tulsa, OK, 74107, USA. FAU - Davis, Randall L AU - Davis RL AD - Oklahoma State University-Center for Health Sciences, Tulsa, OK, 74107, USA. randall.davis@okstate.edu. LA - eng GR - 8P20GM103447/National Institute of General Medical Sciences/ GR - HR12-196/Oklahoma Center for the Advancement of Science and Technology/ PT - Journal Article DEP - 20180720 PL - United States TA - Neurochem Res JT - Neurochemical research JID - 7613461 RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (RNA, Messenger) RN - 4TI98Z838E (Estradiol) SB - IM MH - Animals MH - Brain/*immunology/metabolism MH - Estradiol/metabolism MH - Female MH - Glial Fibrillary Acidic Protein/metabolism MH - Inflammation/immunology MH - Obesity/*etiology/immunology MH - Ovariectomy/*adverse effects MH - Paraventricular Hypothalamic Nucleus/immunology/*metabolism MH - RNA, Messenger/metabolism MH - Rats, Sprague-Dawley MH - Weight Gain/*immunology OTO - NOTNLM OT - Arcuate nucleus OT - Dorsal vagal complex OT - IL-6 OT - MCP-1 OT - Paraventricular nucleus OT - TLR4 EDAT- 2018/07/22 06:00 MHDA- 2018/11/22 06:00 CRDT- 2018/07/22 06:00 PHST- 2018/04/30 00:00 [received] PHST- 2018/07/16 00:00 [accepted] PHST- 2018/06/15 00:00 [revised] PHST- 2018/07/22 06:00 [pubmed] PHST- 2018/11/22 06:00 [medline] PHST- 2018/07/22 06:00 [entrez] AID - 10.1007/s11064-018-2596-6 [pii] AID - 10.1007/s11064-018-2596-6 [doi] PST - ppublish SO - Neurochem Res. 2018 Sep;43(9):1802-1813. doi: 10.1007/s11064-018-2596-6. Epub 2018 Jul 20.