PMID- 30030840 OWN - NLM STAT- MEDLINE DCOM- 20191126 LR - 20191126 IS - 1943-3670 (Electronic) IS - 0022-3492 (Linking) VI - 90 IP - 1 DP - 2019 Jan TI - BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment of ligature-induced periodontitis in dogs. PG - 98-109 LID - 10.1002/JPER.18-0070 [doi] AB - BACKGROUND: Recently, brain-derived neurotrophic factor (BDNF)/high molecular weight hyaluronic acid (HMW-HA) complex with flap surgery has been shown to promote periodontal tissue regeneration. The objective of this study was to evaluate the effects of local subgingival application of BDNF/HMW-HA complex adjunctive to scaling and root planning (SRP) on ligature-induced periodontitis in dogs. METHODS: The dogs were divided into four treatment groups: no treatment (control), SRP alone, SRP followed by local application of HMW-HA (SRP+HMW-HA), and SRP followed by local application of BDNF (500 mug/ml)/ HMW-HA complex (SRP+BDNF/HMW-HA). HMW-HA or BDNF/HMW-HA complex was topically applied to periodontal pockets using a syringe without surgery. Two weeks after treatment, clinical parameters (gingival index, clinical attachment level, periodontal pocket depth and bleeding on probing) were recorded and specimens were collected from anesthetized animals for histological analysis. RESULTS: The SRP+BDNF/HMW-HA group showed significant improvement in all clinical parameters compared to other treatment groups. Histologic analysis showed greater suppression of apical migration of epithelial tissue and milder inflammatory cell infiltration in the SRP+BDNF/HMW-HA group than in the other treatment groups. Furthermore, new cementum and alveolar bone were regenerated, and collagen fibers were inserted into them in the SRP+BDNF/HMW-HA group. CONCLUSION: BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment has the potential to reduce excess inflammation. Further investigation will be needed to clarify periodontal tissue regenerative effects of BDNF/HMW-HA complex in a nonsurgical setting. CI - (c) 2018 American Academy of Periodontology. FAU - Sasaki, Shinya AU - Sasaki S AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Takeda, Katsuhiro AU - Takeda K AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Takewaki, Manabu AU - Takewaki M AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Ouhara, Kazuhisa AU - Ouhara K AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Kajiya, Mikihito AU - Kajiya M AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Mizuno, Noriyoshi AU - Mizuno N AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Fujita, Tsuyoshi AU - Fujita T AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Kurihara, Hidemi AU - Kurihara H AD - Department of Periodontal Medicine, Graduate school of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. LA - eng GR - JP25463218/Japan Society for the Promotion of Science/International PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180830 PL - United States TA - J Periodontol JT - Journal of periodontology JID - 8000345 RN - 0 (Brain-Derived Neurotrophic Factor) SB - IM MH - Animals MH - *Brain-Derived Neurotrophic Factor MH - Dental Scaling MH - Dogs MH - Periodontal Attachment Loss MH - Periodontal Pocket MH - *Periodontitis MH - Root Planing OTO - NOTNLM OT - cytokine OT - periodontitis OT - root planing OT - scaling EDAT- 2018/07/22 06:00 MHDA- 2019/11/27 06:00 CRDT- 2018/07/22 06:00 PHST- 2018/01/31 00:00 [received] PHST- 2018/05/14 00:00 [revised] PHST- 2018/05/16 00:00 [accepted] PHST- 2018/07/22 06:00 [pubmed] PHST- 2019/11/27 06:00 [medline] PHST- 2018/07/22 06:00 [entrez] AID - 10.1002/JPER.18-0070 [doi] PST - ppublish SO - J Periodontol. 2019 Jan;90(1):98-109. doi: 10.1002/JPER.18-0070. Epub 2018 Aug 30.