PMID- 30031708
OWN - NLM
STAT- MEDLINE
DCOM- 20180918
LR  - 20181202
IS  - 1872-7786 (Electronic)
IS  - 0009-2797 (Linking)
VI  - 293
DP  - 2018 Sep 25
TI  - Artesunate ameliorates high glucose-induced rat glomerular mesangial cell injury 
      by suppressing the TLR4/NF-kappaB/NLRP3 inflammasome pathway.
PG  - 11-19
LID - S0009-2797(18)30795-6 [pii]
LID - 10.1016/j.cbi.2018.07.011 [doi]
AB  - Inflammatory response is important for the development and progression of 
      diabetic nephropathy (DN). Artesunate (ART), an antimalarial drug, possesses 
      anti-inflammatory effect and exhibits protective effect on chronic kidney 
      diseases. However, the effect of ART on DN is unknown. The aim of the present 
      study was to evaluate the effect and the molecular mechanism of ART on DN in an 
      in vitro model. The rat mesangial cell line, HBZY-1, was induced by high glucose 
      (HG; 30 mM d-glucose) in the presence or absence of ART (15 and 30 mug/ml) and 
      incubated for 24 h. We found that HG induced the proliferation of HBZY-1 cells, 
      while treatment with ART inhibited the cell proliferation. Treatment with ART 
      inhibited HG-induced inflammatory cytokines production and expression of 
      extracellular matrix (ECM). Besides, HG induced reactive oxygen species (ROS) and 
      malondialdehyde (MDA) levels, and inhibited the superoxide dismutase (SOD) 
      activity of HBZY-1 cells, and the effects were attenuated by ART treatment. ART 
      decreased HG-induced the expression levels of toll-like receptor 4 (TLR4), 
      myeloid differentiation primary response gene 88 (MyD88), nuclear factor kappaB 
      (NF-kappaB) p-p65, and nod-like receptor protein 3 (NLRP3). Inhibition of the 
      TLR4/NF-kappaB pathway suppressed NLRP3 inflammasome in HBZY-1 cells. In conclusion, 
      ART exhibited protective effect on HG-induced HBZY-1 cells by inhibiting the 
      inflammatory response, oxidative stress and ECM accumulation. The 
      TLR4/NF-kappaB/NLRP3 inflammasome pathway was involved in the protective effect of 
      ART. The results suggested that ART might be a potential therapy agent for the DN 
      treatment.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Sun, Zhiqiang
AU  - Sun Z
AD  - Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, 475000, 
      Henan, PR China.
FAU - Ma, Yali
AU  - Ma Y
AD  - Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, 475000, 
      Henan, PR China.
FAU - Chen, Fang
AU  - Chen F
AD  - Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, 475000, 
      Henan, PR China.
FAU - Wang, Shiying
AU  - Wang S
AD  - Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, 475000, 
      Henan, PR China.
FAU - Chen, Baoping
AU  - Chen B
AD  - Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, 475000, 
      Henan, PR China.
FAU - Shi, Jun
AU  - Shi J
AD  - Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, 475000, 
      Henan, PR China. Electronic address: shijun_hh@163.com.
LA  - eng
PT  - Journal Article
DEP - 20180719
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Antimalarials)
RN  - 0 (Artemisinins)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Myd88 protein, rat)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 60W3249T9M (Artesunate)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Antimalarials/*pharmacology
MH  - Artemisinins/*pharmacology
MH  - Artesunate
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Glucose/toxicity
MH  - Inflammasomes/drug effects/*metabolism
MH  - Interleukin-1beta/analysis
MH  - Interleukin-6/analysis
MH  - Malondialdehyde/metabolism
MH  - Mesangial Cells/cytology/drug effects/metabolism
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
MH  - NF-kappa B/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/*drug effects
MH  - Superoxide Dismutase/metabolism
MH  - Toll-Like Receptor 4/metabolism
MH  - Tumor Necrosis Factor-alpha/analysis
OTO - NOTNLM
OT  - Artesunate
OT  - Diabetic nephropathy
OT  - Extracellular matrix accumulation
OT  - Inflammation
OT  - Oxidative stress
EDAT- 2018/07/23 06:00
MHDA- 2018/09/19 06:00
CRDT- 2018/07/23 06:00
PHST- 2018/06/18 00:00 [received]
PHST- 2018/07/11 00:00 [revised]
PHST- 2018/07/16 00:00 [accepted]
PHST- 2018/07/23 06:00 [pubmed]
PHST- 2018/09/19 06:00 [medline]
PHST- 2018/07/23 06:00 [entrez]
AID - S0009-2797(18)30795-6 [pii]
AID - 10.1016/j.cbi.2018.07.011 [doi]
PST - ppublish
SO  - Chem Biol Interact. 2018 Sep 25;293:11-19. doi: 10.1016/j.cbi.2018.07.011. Epub 
      2018 Jul 19.