PMID- 30033329
OWN - NLM
STAT- MEDLINE
DCOM- 20181116
LR  - 20181116
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 214
IP  - 9
DP  - 2018 Sep
TI  - MiR-223-3p overexpression inhibits cell proliferation and migration by regulating 
      inflammation-associated cytokines in glioblastomas.
PG  - 1330-1339
LID - S0344-0338(18)30145-6 [pii]
LID - 10.1016/j.prp.2018.05.012 [doi]
AB  - Glioblastoma(GBM) is most common brain tumor in adults. Currently standard 
      treatments have limited effect to increase the survival, because there are still 
      largely unclear mechanisms in glioblastoma development. miR-223 was involved in 
      various types of cancer, however, the function of miR-223-3p in GBM was still 
      unclear. In our study, real-time PCR was performed to exam the expression level 
      of miR-223-3p and NLRP3 (Nucleotide-binding oligomerization domain(NOD)-like 
      receptor family PYRIN domain containing-3) in GBM tissues. Following that, mimic 
      or inhibitor of miR-223-3p were used to modulate miR-223-3p expression in GBM 
      cell lines respectively. Then, we analyzed cell proliferation and migration by 
      cell counting kit and transwell assay. Further, western blot was performed to 
      detect several inflammation-associated cytokines level in GBM cell lines. We 
      found that miR-223-3p was decreased but NLRP3 was increased in GBM tissues. 
      Treatment with miR-223-3p mimic inhibits cell proliferation and migration via 
      decreasing several inflammation-associated cytokines, including interleukin-1beta 
      (IL-1beta), monocyte chemoattractant protein-1 (MCP-1), IL-8 and IL-18. Importantly, 
      these effects induced by miR-223-3p could be attenuated by NLRP3 overexpression, 
      which was considered as one of target genes of miR-223-3p. In conclusion, these 
      results indicated that miR-223-3p might act as a suppressor and a potential 
      therapy target of GBM.
CI  - Copyright (c) 2018. Published by Elsevier GmbH.
FAU - Ding, Qiuping
AU  - Ding Q
AD  - Department of Surgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 313000, 
      Zhejiang, China.
FAU - Shen, Liang
AU  - Shen L
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Nie, Xiaohu
AU  - Nie X
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Lu, Bin
AU  - Lu B
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Pan, Xuyan
AU  - Pan X
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Su, Zhongzhou
AU  - Su Z
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Yan, Ai
AU  - Yan A
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Yan, Renfu
AU  - Yan R
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Zhou, Yue
AU  - Zhou Y
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China.
FAU - Li, Liqin
AU  - Li L
AD  - Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, 
      Zhejiang, China.
FAU - Xu, Jie
AU  - Xu J
AD  - Department of Neurosurgery, Huzhou Central Hospital, Hongqi Road 198, Huzhou, 
      313000, Zhejiang, China. Electronic address: chzxujie@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20180517
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Cytokines)
RN  - 0 (MIRN223 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRP3 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Brain Neoplasms/*pathology
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Cytokines/*biosynthesis/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Glioblastoma/*pathology
MH  - Humans
MH  - Inflammation/metabolism
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/biosynthesis
OTO - NOTNLM
OT  - GBM
OT  - Inflammation
OT  - MiR-223-3p
OT  - NLRP3
EDAT- 2018/07/24 06:00
MHDA- 2018/11/18 06:00
CRDT- 2018/07/24 06:00
PHST- 2018/02/10 00:00 [received]
PHST- 2018/05/01 00:00 [revised]
PHST- 2018/05/15 00:00 [accepted]
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2018/11/18 06:00 [medline]
PHST- 2018/07/24 06:00 [entrez]
AID - S0344-0338(18)30145-6 [pii]
AID - 10.1016/j.prp.2018.05.012 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2018 Sep;214(9):1330-1339. doi: 10.1016/j.prp.2018.05.012. Epub 
      2018 May 17.