PMID- 30033721 OWN - NLM STAT- MEDLINE DCOM- 20190408 LR - 20190408 IS - 1520-6882 (Electronic) IS - 0003-2700 (Linking) VI - 90 IP - 16 DP - 2018 Aug 21 TI - Upconversion Fluorescent Aptasensor for Polychlorinated Biphenyls Detection Based on Nicking Endonuclease and Hybridization Chain Reaction Dual-Amplification Strategy. PG - 9936-9942 LID - 10.1021/acs.analchem.8b02159 [doi] AB - A novel upconversion fluorescent aptasensor based on hybridization chain reaction and nicking endonuclease has been developed for detection of polychlorinated biphenyls (PCBs). It combined the dual advantages of UCNPs and HCR. Two harpins (H1 and H2) were first designed according to the partial complementary sequence (cDNA) of the PCB72/106. Since the aptamer specifically recognized the target, the cDNA was detached from the magnetic microspheres (MMPs). The cDNA could initiate hybridization chain reaction (HCR) and open the stems of H1 and H2. After the addition of nicking endonuclease, UCNPs were further away from the quenchers (BHQ-1). Hence, the fluorescence intensity of upconversion nanoparticals (UCNPs) could be restored via fluorescence resonance energy transfer (FRET). Therefore, the fluorescence of UCNPs was directly proportional to concentration of PCB72/106, which was the basis for the quantification of PCB72/106. PCB72/106 could be analyzed within the ranges of 0.004 to 800 ng/mL with a detection limit of 0.0035 ng/mL ( S/ N = 3). The aptasensor was also used for the detection of water and soil samples, and the average recoveries ranged from 93.4% to 109.7% and 83.2% to 118.5%, respectively. The relative standard deviations (RSDs) were all below 3.2%. The signal was first amplified through HCR and further amplified with the help of nicking endonuclease. This work also provided the opportunity to develop fluorescent aptasensors for other targets using this dual-amplification strategy. FAU - Wang, Yu AU - Wang Y AUID- ORCID: 0000-0001-7285-8004 AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Bai, Jialei AU - Bai J AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Huo, Bingyang AU - Huo B AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. AD - College of Food Science and Engineering , Jilin University , Changchun 130022 , People's Republic of China. FAU - Yuan, Shuai AU - Yuan S AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Zhang, Man AU - Zhang M AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Sun, Xuan AU - Sun X AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Peng, Yuan AU - Peng Y AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Li, Shuang AU - Li S AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Wang, Jiang AU - Wang J AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Ning, Baoan AU - Ning B AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. FAU - Gao, Zhixian AU - Gao Z AD - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science , Academy of Military Science . Tianjin 300050 , People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180803 PL - United States TA - Anal Chem JT - Analytical chemistry JID - 0370536 RN - 0 (Aptamers, Nucleotide) RN - 9007-49-2 (DNA) RN - DFC2HB4I0K (Polychlorinated Biphenyls) RN - EC 3.1.- (Endodeoxyribonucleases) SB - IM MH - Aptamers, Nucleotide/*chemistry/genetics MH - Base Sequence MH - Biosensing Techniques/*methods MH - DNA/*chemistry/genetics MH - Endodeoxyribonucleases/*chemistry MH - Inverted Repeat Sequences MH - Limit of Detection MH - Metal Nanoparticles/chemistry MH - Nucleic Acid Amplification Techniques/*methods MH - Nucleic Acid Hybridization MH - Polychlorinated Biphenyls/*analysis EDAT- 2018/07/24 06:00 MHDA- 2019/04/09 06:00 CRDT- 2018/07/24 06:00 PHST- 2018/07/24 06:00 [pubmed] PHST- 2019/04/09 06:00 [medline] PHST- 2018/07/24 06:00 [entrez] AID - 10.1021/acs.analchem.8b02159 [doi] PST - ppublish SO - Anal Chem. 2018 Aug 21;90(16):9936-9942. doi: 10.1021/acs.analchem.8b02159. Epub 2018 Aug 3.