PMID- 30033772
OWN - NLM
STAT- MEDLINE
DCOM- 20190618
LR  - 20190618
IS  - 2169-141X (Electronic)
IS  - 2169-1401 (Linking)
VI  - 46
IP  - sup3
DP  - 2018
TI  - Poly-l-lysine-coated superparamagnetic nanoparticles: a novel method for the 
      transfection of pro-BDNF into neural stem cells.
PG  - S125-S132
LID - 10.1080/21691401.2018.1489272 [doi]
AB  - Poly-l-lysine-coated superparamagnetic iron oxide nanoparticles (SPIONs-PLL) were 
      prepared and used as a novel-carrier for the transfer of brain-derived 
      neurotrophic factor (BDNF) into neural stem cells (NSCs) under the beneficial 
      influence of an external magnetic field. Pro-BDNF, a gene from human brain cDNA 
      libraries, was obtained by polymerase chain reaction and constructed in a 
      mammalian expression vector (PSecTag2/HygroB). The nanoparticles (NPs) were 
      examined using Fourier transform infrared spectroscopy, zeta potential, and 
      Transmission electron microscopy. From the results, the levels of BDNF among the 
      transfected and untransfected cells were 30.326 +/- 5.9 and 5.85 +/- 3.11 pg/mL, 
      respectively, as detected by an ELISA method. Moreover, the enhanced green 
      fluorescent protein vector was used to evaluate the gene expression efficiency 
      for SPIONs-PLL as a non-viral carrier in NSCs. This was performed under the 
      influence of a magnetic field and the transfection reagents (such as 
      Lipofectamine 2000), which served as a positive control. The histological 
      analysis revealed that the concentration of intracellular NPs was significantly 
      higher than intercellular NPs. These results suggest that SPIONs-PLL can serve as 
      a novel alternative for the transfection of BDNF-NSCs and could be used in gene 
      therapy.
FAU - Albukhaty, Salim
AU  - Albukhaty S
AD  - a Department of Basic Science, College of Nursing , University of Misan , Maysan 
      , Iraq.
FAU - Naderi-Manesh, Hossein
AU  - Naderi-Manesh H
AD  - b Department of Nanobiotechnology, Faculty of Biological Sciences , Tarbiat 
      Modares University , Tehran , Iran.
FAU - Tiraihi, Taki
AU  - Tiraihi T
AD  - c Department of Anatomical Science , Tarbiat Modares University , Tehran , Iran.
FAU - Sakhi Jabir, Majid
AU  - Sakhi Jabir M
AD  - d Division of Biotechnology , University of technology , Baghdad , Iraq ; 
      Department of Biotechnology , University of technology , Baghdad , Iraq.
LA  - eng
PT  - Journal Article
DEP - 20180722
PL  - England
TA  - Artif Cells Nanomed Biotechnol
JT  - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Protein Precursors)
RN  - 0 (brain-derived neurotrophic factor precursor)
RN  - 25104-18-1 (Polylysine)
SB  - IM
MH  - Animals
MH  - *Brain-Derived Neurotrophic Factor/biosynthesis/genetics
MH  - Genetic Therapy/methods
MH  - Magnetite Nanoparticles/*chemistry
MH  - Neural Stem Cells/cytology/*metabolism
MH  - *Polylysine/chemistry/pharmacology
MH  - *Protein Precursors/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transfection/*methods
OTO - NOTNLM
OT  - NSCs
OT  - SPIONs-PLL
OT  - pro-BDNF
OT  - transfection
EDAT- 2018/07/24 06:00
MHDA- 2019/06/19 06:00
CRDT- 2018/07/24 06:00
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2019/06/19 06:00 [medline]
PHST- 2018/07/24 06:00 [entrez]
AID - 10.1080/21691401.2018.1489272 [doi]
PST - ppublish
SO  - Artif Cells Nanomed Biotechnol. 2018;46(sup3):S125-S132. doi: 
      10.1080/21691401.2018.1489272. Epub 2018 Jul 22.