PMID- 30034568
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1932-1058 (Print)
IS  - 1932-1058 (Electronic)
IS  - 1932-1058 (Linking)
VI  - 12
IP  - 4
DP  - 2018 Jul
TI  - Biological characterization of the modified poly(dimethylsiloxane) surfaces based 
      on cell attachment and toxicity assays.
PG  - 044105
LID - 10.1063/1.5035176 [doi]
LID - 044105
AB  - Poly(dimethylsiloxane) (PDMS) is a material applicable for tissue and biomedical 
      engineering, especially based on microfluidic devices. PDMS is a material used in 
      studies aimed at understanding cell behavior and analyzing the cell adhesion 
      mechanism. In this work, biological characterization of the modified PDMS 
      surfaces based on cell attachment and toxicity assays was performed. We studied 
      Balb 3T3/c, HMEC-1, and HT-29 cell adhesion on poly(dimethylsiloxane) surfaces 
      modified by different proteins, with and without pre-activation with plasma 
      oxygen and UV irradiation. Additionally, we studied how changing of a base and a 
      curing agent ratios influence cell proliferation. We observed that cell type has 
      a high impact on cell adhesion, proliferation, as well as viability after drug 
      exposure. It was tested that the carcinoma cells do not require a highly specific 
      microenvironment for their proliferation. Cytotoxicity assays with celecoxib and 
      oxaliplatin on the modified PDMS surfaces showed that normal cells, cultured on 
      the modified PDMS, are more sensitive to drugs than cancer cells. Cell adhesion 
      was also tested in the microfluidic systems made of the modified PDMS layers. 
      Thanks to that, we studied how the surface area to volume ratio influences cell 
      behavior. The results presented in this manuscript could be helpful for creation 
      of proper culture conditions during in vitro tests as well as to understand cell 
      response in different states of disease depending on drug exposure.
FAU - Jastrzebska, Elzbieta
AU  - Jastrzebska E
AD  - Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of 
      Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
FAU - Zuchowska, Agnieszka
AU  - Zuchowska A
AD  - Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of 
      Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
FAU - Flis, Sylwia
AU  - Flis S
AD  - Department of Pharmacology, National Medicines Institute, Chelmska 30/34, 00-725 
      Warsaw, Poland.
FAU - Sokolowska, Patrycja
AU  - Sokolowska P
AD  - Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of 
      Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
FAU - Bulka, Magdalena
AU  - Bulka M
AD  - Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of 
      Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
FAU - Dybko, Artur
AU  - Dybko A
AD  - Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of 
      Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
FAU - Brzozka, Zbigniew
AU  - Brzozka Z
AD  - Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of 
      Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20180710
PL  - United States
TA  - Biomicrofluidics
JT  - Biomicrofluidics
JID - 101293825
PMC - PMC6039296
EDAT- 2018/07/24 06:00
MHDA- 2018/07/24 06:01
PMCR- 2019/07/10
CRDT- 2018/07/24 06:00
PHST- 2018/04/13 00:00 [received]
PHST- 2018/06/17 00:00 [accepted]
PHST- 2018/07/24 06:00 [entrez]
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2018/07/24 06:01 [medline]
PHST- 2019/07/10 00:00 [pmc-release]
AID - 1.5035176 [pii]
AID - 004804BMF [pii]
AID - 10.1063/1.5035176 [doi]
PST - epublish
SO  - Biomicrofluidics. 2018 Jul 10;12(4):044105. doi: 10.1063/1.5035176. eCollection 
      2018 Jul.