PMID- 30036111
OWN - NLM
STAT- MEDLINE
DCOM- 20191001
LR  - 20191001
IS  - 1557-7465 (Electronic)
IS  - 1079-9907 (Linking)
VI  - 38
IP  - 8
DP  - 2018 Aug
TI  - KLF15 Regulates the Expression of MMP-3 in Human Chondrocytes.
PG  - 356-362
LID - 10.1089/jir.2017.0135 [doi]
AB  - Imbalance of metabolism on catabolic and anabolic molecules in chondrocytes has 
      been associated with the cartilage damage in osteoarthritis (OA). Matrix 
      metalloproteinase-3 (MMP-3), one of the most important catabolic factors, acts as 
      a cartilage-degrading enzyme, which is associated with the degradation of type II 
      collagen and aggrecan. Kruppel-like factor 15 (KLF15), an important member of the 
      KLFs family, possesses a variety of biological functions. However, the 
      physiological roles of KLF15 in chondrocytes and the pathological progression of 
      OA remain unknown. In the current study, we report that KLF15 is expressed in 
      primary chondrocytes as well as ATDC5 and SW1353 chondrogenic cell lines. 
      Interestingly, KLF15 expression was significantly lower in chondrocytes from OA 
      patients compared with those from normal subjects. Also, we found that tumor 
      necrosis factor alpha (TNF-alpha) treatment reduced the expression of KLF15 mediated by 
      p53 in human chondrocytes. Notably, it was shown that KLF15 reduced TNF-alpha-induced 
      expression of MMP-3 at the transcriptional level. Mechanistically, the chromatin 
      immunoprecipitation assay displayed that KLF15 could bind to the promoter region 
      of MMP-3. Our results suggest that KLF15 might be a novel therapeutic target of 
      OA.
FAU - Li, Yishuo
AU  - Li Y
AD  - 1 Department of Rheumatology and Immunology, The First Hospital of China Medical 
      University , Shenyang, China .
FAU - Zhao, Min
AU  - Zhao M
AD  - 2 Department of Rheumatology and Immunology, The First Affiliated Hospital of 
      Dalian Medical University , Dalian, China .
FAU - Xiao, Weiguo
AU  - Xiao W
AD  - 1 Department of Rheumatology and Immunology, The First Hospital of China Medical 
      University , Shenyang, China .
LA  - eng
PT  - Journal Article
DEP - 20180723
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (KLF15 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Cells, Cultured
MH  - Chondrocytes/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Kruppel-Like Transcription Factors/antagonists & inhibitors/genetics/*metabolism
MH  - Matrix Metalloproteinase 3/*genetics/metabolism
MH  - Nuclear Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Osteoarthritis/drug therapy/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
OTO - NOTNLM
OT  - Kruppel-like factor 15
OT  - chondrocytes
OT  - matrix metalloproteinase-3
OT  - tumor necrosis factor alpha
EDAT- 2018/07/24 06:00
MHDA- 2019/10/02 06:00
CRDT- 2018/07/24 06:00
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2019/10/02 06:00 [medline]
PHST- 2018/07/24 06:00 [entrez]
AID - 10.1089/jir.2017.0135 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2018 Aug;38(8):356-362. doi: 10.1089/jir.2017.0135. 
      Epub 2018 Jul 23.