PMID- 30036465
OWN - NLM
STAT- MEDLINE
DCOM- 20190917
LR  - 20190917
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 52
IP  - 16
DP  - 2018 Aug 21
TI  - In Situ Real-Time Study on Dynamics of Microbially Induced Calcium Carbonate 
      Precipitation at a Single-Cell Level.
PG  - 9266-9276
LID - 10.1021/acs.est.8b02660 [doi]
AB  - Ureolytic microbially induced calcium carbonate precipitation (MICP) is a 
      promising green technique for addressing a variety of environmental and 
      architectural concerns. However, the dynamics of MICP especially at the 
      microscopic level remains relatively unexplored. In this work, by applying a 
      bacterial tracking technique, the growth dynamics of micrometer-sized calcium 
      carbonate precipitates induced by Sporosarcina pasteurii were studied at a 
      single-cell resolution. The growth of micrometer-scale precipitates and the 
      occurrence and dissolution of many unstable submicrometer calcium carbonate 
      particles were observed in the precipitation process. More interestingly, we 
      observed that micrometer-sized precipitated crystals did not grow on negatively 
      charged cell surfaces nor on other tested polystyrene microspheres with different 
      negatively charged surface modifications, indicating that a negatively charged 
      surface was not a sufficient property for nucleating the growth of precipitates 
      in the MICP process under the conditions used in this study. Our observations 
      imply that the frequently cited model of bacterial cell surfaces as nucleation 
      sites for precipitates during MICP is oversimplified. In addition, additional 
      growth of calcium carbonates was observed on old precipitates collected from 
      previous runs. The presence of bacterial cells was also shown to affect both 
      morphologies and crystalline structures of precipitates, and both calcite and 
      vaterite precipitates were found when cells physically coexisted with 
      precipitates. This study provides new insights into the regulation of MICP 
      through dynamic control of precipitation.
FAU - Zhang, Wenchao
AU  - Zhang W
AD  - Key Laboratory of Systems Bioengineering (Ministry of Education), School of 
      Chemical Engineering and Technology , Tianjin University , Tianjin 300072 , 
      China.
FAU - Ju, Ying
AU  - Ju Y
AD  - Key Laboratory of Systems Bioengineering (Ministry of Education), School of 
      Chemical Engineering and Technology , Tianjin University , Tianjin 300072 , 
      China.
FAU - Zong, Yiwu
AU  - Zong Y
AD  - Key Laboratory of Systems Bioengineering (Ministry of Education), School of 
      Chemical Engineering and Technology , Tianjin University , Tianjin 300072 , 
      China.
FAU - Qi, Hao
AU  - Qi H
AD  - Key Laboratory of Systems Bioengineering (Ministry of Education), School of 
      Chemical Engineering and Technology , Tianjin University , Tianjin 300072 , 
      China.
FAU - Zhao, Kun
AU  - Zhao K
AUID- ORCID: 0000-0003-3928-1981
AD  - Key Laboratory of Systems Bioengineering (Ministry of Education), School of 
      Chemical Engineering and Technology , Tianjin University , Tianjin 300072 , 
      China.
AD  - Collaborative Innovation Centre of Chemical Science and Engineering , Tianjin 
      University , Tianjin 300072 , China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180806
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - *Calcium Carbonate
MH  - Chemical Precipitation
MH  - *Sporosarcina
MH  - Time and Motion Studies
EDAT- 2018/07/24 06:00
MHDA- 2019/09/19 06:00
CRDT- 2018/07/24 06:00
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2019/09/19 06:00 [medline]
PHST- 2018/07/24 06:00 [entrez]
AID - 10.1021/acs.est.8b02660 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2018 Aug 21;52(16):9266-9276. doi: 10.1021/acs.est.8b02660. 
      Epub 2018 Aug 6.