PMID- 30036686
OWN - NLM
STAT- MEDLINE
DCOM- 20180904
LR  - 20181202
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 296
DP  - 2018 Oct 15
TI  - Placental mechanism of prenatal nicotine exposure-reduced blood cholesterol 
      levels in female fetal rats.
PG  - 31-38
LID - S0378-4274(18)31509-1 [pii]
LID - 10.1016/j.toxlet.2018.07.022 [doi]
AB  - Clinical studies showed that intrauterine growth retardation (IUGR) neonatus had 
      lower cholesterol concentrations in cord blood, which might be associated with 
      increased risk of metabolic syndrome and cardiovascular diseases in adulthood. We 
      previously observed lower blood cholesterol levels in prenatal nicotine exposure 
      (PNE)-induced IUGR fetal rats, and this study aimed to elucidate the placental 
      mechanism. Pregnant Wistar rats were subcutaneously injected with nicotine 
      (2.0 mg/kg⋅d) on gestational day 9-20. In vivo, PNE increased levels of total 
      cholesterol (TCH), high-density lipoprotein-cholesterol (HDL-C) and low-density 
      lipoprotein-cholesterol (LDL-C) in maternal serum, while decreased levels of TCH 
      and LDL-C in female fetal serum. Meanwhile, the expression of scavenger receptor 
      class B type 1 (SR-B1), ATP-binding cassette transporter A1 (ABCA1) and 
      ATP-binding cassette transporter G1 (ABCG1) were decreased, and the expression of 
      liver X receptor (LXR) alpha and beta were also decreased in female placentas. In vitro, 
      nicotine (0.1-10 muM) reduced the expression of LXRalpha, LXRbeta, SR-B1, ABCA1 and ABCG1 
      in a concentration dependent manner, which could be annulled by nAChR antagonist 
      and LXR agonist. Taken together, nicotine could inhibit the expression of SR-B1, 
      ABCA1 and ABCG1 via nAChR and LXR alpha/beta in female placentas, finally leading to 
      reduced blood cholesterol levels in fetal rats.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Zhang, Guohui
AU  - Zhang G
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      169 Donghu Road, Wuchang District, Wuhan 430071, China.
FAU - Zhou, Jin
AU  - Zhou J
AD  - Department of Pharmacology, Basic Medical School of Wuhan University, 185 Donghu 
      Road, Wuchang District, Wuhan 430071, China.
FAU - Huang, Wen
AU  - Huang W
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      169 Donghu Road, Wuchang District, Wuhan 430071, China.
FAU - Yu, Luting
AU  - Yu L
AD  - Department of Pharmacology, Basic Medical School of Wuhan University, 185 Donghu 
      Road, Wuchang District, Wuhan 430071, China.
FAU - Zhang, Yuanzhen
AU  - Zhang Y
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      169 Donghu Road, Wuchang District, Wuhan 430071, China; Hubei Provincial Key 
      Laboratory of Developmentally Originated Diseases, 185 Donghu Road, Wuchang 
      District, Wuhan 430071, China. Electronic address: zhangyuanzhen@vip.sina.com.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      169 Donghu Road, Wuchang District, Wuhan 430071, China; Department of 
      Pharmacology, Basic Medical School of Wuhan University, 185 Donghu Road, Wuchang 
      District, Wuhan 430071, China; Hubei Provincial Key Laboratory of Developmentally 
      Originated Diseases, 185 Donghu Road, Wuchang District, Wuhan 430071, China. 
      Electronic address: wanghui19@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180720
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (ABCA1 protein, rat)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (Abcg1 protein, rat)
RN  - 0 (Cholinergic Antagonists)
RN  - 0 (Liver X Receptors)
RN  - 0 (Scarb1 protein, rat)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 6M3C89ZY6R (Nicotine)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - ATP Binding Cassette Transporter 1/biosynthesis
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1/biosynthesis
MH  - Animals
MH  - Cell Line
MH  - Cholesterol/biosynthesis/*blood
MH  - Cholinergic Antagonists/pharmacology
MH  - Female
MH  - Fetal Growth Retardation/chemically induced/pathology
MH  - Fetus/metabolism
MH  - Humans
MH  - Liver X Receptors/agonists/biosynthesis
MH  - Nicotine/*pharmacology
MH  - Placenta/*drug effects/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Scavenger Receptors, Class B/biosynthesis
OTO - NOTNLM
OT  - Cholesterol transport
OT  - Intrauterine growth retardation
OT  - Liver X receptor (LXR)
OT  - Nicotinic acetylcholine receptor
OT  - Placenta
OT  - Prenatal nicotine exposure
EDAT- 2018/07/24 06:00
MHDA- 2018/09/05 06:00
CRDT- 2018/07/24 06:00
PHST- 2018/02/24 00:00 [received]
PHST- 2018/07/09 00:00 [revised]
PHST- 2018/07/19 00:00 [accepted]
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2018/09/05 06:00 [medline]
PHST- 2018/07/24 06:00 [entrez]
AID - S0378-4274(18)31509-1 [pii]
AID - 10.1016/j.toxlet.2018.07.022 [doi]
PST - ppublish
SO  - Toxicol Lett. 2018 Oct 15;296:31-38. doi: 10.1016/j.toxlet.2018.07.022. Epub 2018 
      Jul 20.