PMID- 30036791
OWN - NLM
STAT- MEDLINE
DCOM- 20181112
LR  - 20220409
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 171
DP  - 2018 Nov 1
TI  - The effect of surface charge on the cytotoxicity and uptake of carbon quantum 
      dots in human umbilical cord derived mesenchymal stem cells.
PG  - 241-249
LID - S0927-7765(18)30481-8 [pii]
LID - 10.1016/j.colsurfb.2018.07.034 [doi]
AB  - Carbon quantum dots (CQDs) are emerging as an ideal agent for efficient stem cell 
      labeling. In current study, we synthesized a series of CQDs carrying different 
      surface charges by changing the mass ratio of diammonium citrate (DC) and 
      spermidine (Spd), and evaluated the effects of different surface charges on the 
      cytotoxicity, cellular uptake, stability in human umbilical cord derived 
      mesenchymal stem cells (hUCMSCs). We ascertained the optimal labeling time (24 h) 
      and subtoxic concentration (50 mug/mL) of all different charged CQDs. Our results 
      demonstrated that, although positively charged CQDs are more cytotoxic and have 
      lower photoluminescence (PL) compared to negative CQDs, they still have higher 
      labeling efficiency for their higher uptake capacity. We found that relatively 
      weak positive surface charges enabled CQDs to possess good biocompatibility and 
      labeling efficiency in hUCMSCs. This work will helpfully contribute to the design 
      and optimization of CQDs for tracking stem cells and further benefit to clinical 
      research and application.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Yan, Junyan
AU  - Yan J
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China; School 
      of Life Science, Shaoxing University, Zhejiang, 312000, China.
FAU - Hou, Shulan
AU  - Hou S
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China; School 
      of Pharmacy, Xi'an Jiaotong University, Xi'an, Shannxi, 710061, China.
FAU - Yu, Yanzhen
AU  - Yu Y
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China; 
      University of Science and Technology of China, Hefei, Anhui, 230026, China.
FAU - Qiao, Yong
AU  - Qiao Y
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China.
FAU - Xiao, Tongqian
AU  - Xiao T
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China; 
      University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Mei, Yan
AU  - Mei Y
AD  - Greepharma Inc., Nanjing, Jiangsu, 211100, China.
FAU - Zhang, Zhijun
AU  - Zhang Z
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China.
FAU - Wang, Bin
AU  - Wang B
AD  - Center for Clinic Stem Cell Research, The Affiliated Drum Tower Hospital of 
      Nanjing University Medical School, Nanjing, Jiangsu, 210008, China.
FAU - Huang, Chih-Ching
AU  - Huang CC
AD  - Department of Bioscience and Biotechnology, National Taiwan Ocean University, 
      Keelung, 20224, Taiwan.
FAU - Lin, Chia-Hua
AU  - Lin CH
AD  - Department of Biotechnology, National Formosa University, Yunlin, 63208, Taiwan. 
      Electronic address: vicchlin@nfu.edu.tw.
FAU - Suo, Guangli
AU  - Suo G
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China. 
      Electronic address: glsuo2013@sinano.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20180717
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Reactive Oxygen Species)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Carbon/chemistry/*pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/*drug effects
MH  - Particle Size
MH  - Quantum Dots/*chemistry
MH  - Reactive Oxygen Species/analysis/metabolism
MH  - *Static Electricity
MH  - Surface Properties
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Carbon quantum dots
OT  - Cytotoxicity
OT  - Human umbilical cord derived mesenchymal stem cells
OT  - Labeling efficiency
OT  - Surface charge
EDAT- 2018/07/24 06:00
MHDA- 2018/11/13 06:00
CRDT- 2018/07/24 06:00
PHST- 2018/04/18 00:00 [received]
PHST- 2018/06/22 00:00 [revised]
PHST- 2018/07/16 00:00 [accepted]
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2018/11/13 06:00 [medline]
PHST- 2018/07/24 06:00 [entrez]
AID - S0927-7765(18)30481-8 [pii]
AID - 10.1016/j.colsurfb.2018.07.034 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2018 Nov 1;171:241-249. doi: 
      10.1016/j.colsurfb.2018.07.034. Epub 2018 Jul 17.