PMID- 30036800
OWN - NLM
STAT- MEDLINE
DCOM- 20190327
LR  - 20190327
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 101
DP  - 2018 Sep
TI  - Th17 activation by dendritic cells stimulated with gamma-irradiated Streptococcus 
      pneumoniae.
PG  - 344-352
LID - S0161-5890(18)30576-5 [pii]
LID - 10.1016/j.molimm.2018.07.023 [doi]
AB  - Dendritic cells (DCs) play an important role in antigen presentation, which is an 
      essential step for the induction of antigen-specific adaptive immunity. 
      Inactivated bacterial whole cell vaccines have been widely used to prevent many 
      bacterial infections because they elicit good immunogenicity due to the presence 
      of various antigens and are relatively inexpensive and easy to manufacture. 
      Recently, gamma-irradiated whole cells of nonencapsulated Streptococcus 
      pneumoniae were developed as a broad-spectrum and serotype-independent 
      multivalent vaccine. In the present study, we generated gamma-irradiated S. 
      pneumoniae (r-SP) and investigated its capacity to stimulate mouse bone 
      marrow-derived DCs (BM-DCs) in comparison with heat-inactivated and 
      formalin-inactivated S. pneumoniae (h-SP and f-SP, respectively). r-SP showed an 
      attenuated binding and internalization level to BM-DCs when compared to h-SP or 
      f-SP. r-SP weakly induced the expression of CD80, CD83, CD86, MHC class I, and 
      PD-L2 compared with h-SP or f-SP. Furthermore, r-SP less potently induced IL-6, 
      TNF-alpha, and IL-23 expression than h-SP or f-SP but more potently induced IL-1beta 
      expression than h-SP or f-SP in BM-DCs. Since Th17-mediated immune responses are 
      known to be important for the protection against pneumococcal infections, 
      r-SP-primed DCs were co-cultured with splenocytes or splenic CD4(+) T cells. 
      Interestingly, r-SP-sensitized BM-DCs markedly induced IL-17A(+) CD4(+) T cells 
      whereas h-SP- or f-SP-sensitized BM-DCs weakly induced them. Collectively, these 
      results suggest that r-SP could be an effective pneumococcal vaccine candidate 
      eliciting Th17-mediated immune responses by stimulation of DCs.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Kim, Hyun Young
AU  - Kim HY
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Kim, Sun Kyung
AU  - Kim SK
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Seo, Ho Seong
AU  - Seo HS
AD  - Research Division for Biotechnology, Korea Atomic Energy Research Institute, 
      Jeongeup 56212, Republic of Korea.
FAU - Jeong, Soyoung
AU  - Jeong S
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Ahn, Ki Bum
AU  - Ahn KB
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea; 
      Research Division for Biotechnology, Korea Atomic Energy Research Institute, 
      Jeongeup 56212, Republic of Korea.
FAU - Yun, Cheol-Heui
AU  - Yun CH
AD  - Department of Agricultural Biotechnology and Research Institute for Agriculture 
      and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Han, Seung Hyun
AU  - Han SH
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea. 
      Electronic address: shhan-mi@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180721
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (B7-H1 Antigen)
RN  - 0 (Biomarkers)
RN  - 0 (Cd274 protein, mouse)
RN  - 0 (Cytokines)
RN  - 0 (Pdcd1lg2 protein, mouse)
RN  - 0 (Programmed Cell Death 1 Ligand 2 Protein)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Animals
MH  - B7-H1 Antigen/metabolism
MH  - Bacterial Adhesion/radiation effects
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/immunology
MH  - Cell Differentiation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Endocytosis
MH  - Formaldehyde
MH  - *Gamma Rays
MH  - Hot Temperature
MH  - Lymphocyte Activation/*immunology
MH  - Mice
MH  - Programmed Cell Death 1 Ligand 2 Protein/metabolism
MH  - Receptors, Antigen, T-Cell, gamma-delta/metabolism
MH  - Spleen/metabolism
MH  - Streptococcus pneumoniae/*immunology/*radiation effects
MH  - Th17 Cells/*immunology
OTO - NOTNLM
OT  - Dendritic cell
OT  - Gamma-Irradiation
OT  - Streptococcus pneumoniae
OT  - Th17
OT  - Vaccine
EDAT- 2018/07/24 06:00
MHDA- 2019/03/28 06:00
CRDT- 2018/07/24 06:00
PHST- 2018/04/04 00:00 [received]
PHST- 2018/06/22 00:00 [revised]
PHST- 2018/07/15 00:00 [accepted]
PHST- 2018/07/24 06:00 [pubmed]
PHST- 2019/03/28 06:00 [medline]
PHST- 2018/07/24 06:00 [entrez]
AID - S0161-5890(18)30576-5 [pii]
AID - 10.1016/j.molimm.2018.07.023 [doi]
PST - ppublish
SO  - Mol Immunol. 2018 Sep;101:344-352. doi: 10.1016/j.molimm.2018.07.023. Epub 2018 
      Jul 21.