PMID- 30040218
OWN - NLM
STAT- MEDLINE
DCOM- 20190405
LR  - 20210109
IS  - 1934-368X (Electronic)
IS  - 1934-3671 (Print)
IS  - 1934-3671 (Linking)
VI  - 121
IP  - 1
DP  - 2018 Apr
TI  - Isolation of Murine Skin Resident and Migratory Dendritic Cells via Enzymatic 
      Digestion.
PG  - e45
LID - 10.1002/cpim.45 [doi]
AB  - Dendritic cells (DCs) are a highly specialized subset of professional 
      antigen-presenting cells (APCs) that reside in peripheral and lymphoid tissues. 
      DCs capture antigen in the periphery and migrate to the lymph node where they 
      prime naive T cells. In addition, DCs have been recently appreciated to have 
      function in innate immunity within tissues. In the skin, heterogeneous 
      populations of DCs reside within the epidermis and the dermis. Analysis of the 
      cutaneous DC subsets is complicated by requirements of distinct enzymatic 
      digestion protocols for isolation of APCs from distinct anatomical compartments 
      of the skin. Here, specific approaches for isolation of DCs from the epidermis, 
      dermis, and the skin-draining lymph nodes of mice are described. (c) 2018 by John 
      Wiley & Sons, Inc.
CI  - Copyright (c) 2018 John Wiley & Sons, Inc.
FAU - Kashem, Sakeen W
AU  - Kashem SW
AD  - Center for Immunology, Department of Dermatology, University of Minnesota, 
      Minneapolis, Minnesota.
FAU - Kaplan, Daniel H
AU  - Kaplan DH
AD  - Department of Dermatology, Department of Immunology, University of Pittsburgh, 
      Pittsburgh, Pennsylvania.
LA  - eng
GR  - R01 AR060744/AR/NIAMS NIH HHS/United States
GR  - R01 AR067187/AR/NIAMS NIH HHS/United States
GR  - R01 AR071720/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Curr Protoc Immunol
JT  - Current protocols in immunology
JID - 9101651
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Cell Separation
MH  - Dendritic Cells/*cytology
MH  - Female
MH  - Lymph Nodes/cytology
MH  - Mice
MH  - Skin/*cytology/immunology
PMC - PMC6060630
MID - NIHMS918505
OTO - NOTNLM
OT  - Langerhans cells
OT  - antigen-presenting cell
OT  - dendritic cell
OT  - skin
EDAT- 2018/07/25 06:00
MHDA- 2019/04/06 06:00
PMCR- 2019/04/01
CRDT- 2018/07/25 06:00
PHST- 2018/07/25 06:00 [entrez]
PHST- 2018/07/25 06:00 [pubmed]
PHST- 2019/04/06 06:00 [medline]
PHST- 2019/04/01 00:00 [pmc-release]
AID - 10.1002/cpim.45 [doi]
PST - ppublish
SO  - Curr Protoc Immunol. 2018 Apr;121(1):e45. doi: 10.1002/cpim.45.