PMID- 30041062
OWN - NLM
STAT- MEDLINE
DCOM- 20181002
LR  - 20181004
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 213
DP  - 2018 Nov
TI  - Silencing of HIF-1 in WSSV-infected white shrimp: Effect on viral load and 
      antioxidant enzymes.
PG  - 19-26
LID - S1532-0456(18)30094-2 [pii]
LID - 10.1016/j.cbpc.2018.07.004 [doi]
AB  - Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes 
      involved in glucose metabolism. HIF-1 is formed by a regulatory alpha-subunit 
      (HIF-1alpha) and a constitutive beta-subunit (HIF-1beta). The white spot syndrome virus 
      (WSSV) induces a shift in glucose metabolism and oxidative stress. HIF-1alpha is 
      associated with the induction of metabolic changes in tissues of WSSV-infected 
      shrimp. However, the contributions of HIF-1 to viral load and antioxidant 
      responses in WSSV-infected shrimp have been not examined. In this study, the 
      effect of HIF-1 silencing on viral load and the expression and activity of 
      antioxidant enzymes (superoxide dismutase-SOD, glutathione S-transferase-GST, and 
      catalase) along with oxidative damage (lipid peroxidation and protein carbonyl) 
      in tissues of white shrimp infected with the WSSV were studied. The viral load 
      increased in hepatopancreas and muscle after WSSV infection, and the accumulative 
      mortality was of 100% at 72 h post-infection. The expression and activity of SOD, 
      catalase, and GST decreased in each tissue evaluated after WSSV infection. 
      Protein carbonyl concentrations increased in each tissue after WSSV infection, 
      while lipid peroxidation increased in hepatopancreas, but not in muscle. 
      Silencing of HIF-1alpha decreased the WSSV viral load in hepatopancreas and muscle of 
      infected shrimp along with shrimp mortality. Silencing of HIF-1alpha ameliorated the 
      antioxidant response in a tissue-specific manner, which translated to a decrease 
      in oxidative damage. These results suggest that HIF-1 is essential for restoring 
      the antioxidant response, which counters the oxidative injury associated with 
      WSSV infection.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Miranda-Cruz, Melissa M
AU  - Miranda-Cruz MM
AD  - Universidad de Sonora, Departamento de Ciencias Quimico-Biologicas y 
      Agropecuarias, Universidad de Sonora Unidad Regional Sur, Apartado Postal 85880, 
      Navojoa, Sonora, Mexico.
FAU - Poom-Llamas, Jennifer J
AU  - Poom-Llamas JJ
AD  - Universidad de Sonora, Departamento de Ciencias Quimico-Biologicas y 
      Agropecuarias, Universidad de Sonora Unidad Regional Sur, Apartado Postal 85880, 
      Navojoa, Sonora, Mexico.
FAU - Godoy-Lugo, Jose A
AU  - Godoy-Lugo JA
AD  - School of Natural Sciences, University of California Merced, 5200 N Lake Road, 
      Merced, CA 95343, USA.
FAU - Ortiz, Rudy M
AU  - Ortiz RM
AD  - School of Natural Sciences, University of California Merced, 5200 N Lake Road, 
      Merced, CA 95343, USA.
FAU - Gomez-Jimenez, Silvia
AU  - Gomez-Jimenez S
AD  - Centro de Investigacion en Alimentacion y Desarrollo (CIAD), Carretera a la 
      Victoria KM. 0.6. Hermosillo, Sonora, C.P. 83304, Mexico.
FAU - Rosas-Rodriguez, Jesus A
AU  - Rosas-Rodriguez JA
AD  - Universidad de Sonora, Departamento de Ciencias Quimico-Biologicas y 
      Agropecuarias, Universidad de Sonora Unidad Regional Sur, Apartado Postal 85880, 
      Navojoa, Sonora, Mexico.
FAU - Moran-Palacio, Edgar F
AU  - Moran-Palacio EF
AD  - Universidad de Sonora, Departamento de Ciencias Quimico-Biologicas y 
      Agropecuarias, Universidad de Sonora Unidad Regional Sur, Apartado Postal 85880, 
      Navojoa, Sonora, Mexico.
FAU - Sonanez-Organis, Jose G
AU  - Sonanez-Organis JG
AD  - Universidad de Sonora, Departamento de Ciencias Quimico-Biologicas y 
      Agropecuarias, Universidad de Sonora Unidad Regional Sur, Apartado Postal 85880, 
      Navojoa, Sonora, Mexico. Electronic address: jorganis@navojoa.uson.mx.
LA  - eng
PT  - Journal Article
DEP - 20180721
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (DNA, Viral)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Double-Stranded)
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - DNA, Viral/isolation & purification
MH  - *Gene Expression Regulation, Developmental
MH  - Gene Silencing
MH  - Hepatopancreas/growth & development/metabolism/virology
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*antagonists & 
      inhibitors/genetics/metabolism
MH  - Injections, Intramuscular
MH  - Lipid Peroxidation
MH  - Mexico
MH  - Muscles/metabolism/virology
MH  - Organ Specificity
MH  - Oxidative Stress
MH  - Oxidoreductases/genetics/metabolism
MH  - Penaeidae/growth & development/metabolism/*virology
MH  - Protein Carbonylation
MH  - RNA Interference
MH  - RNA, Double-Stranded/administration & dosage/metabolism
MH  - Viral Load
MH  - White spot syndrome virus 1/isolation & purification/*pathogenicity/physiology
OTO - NOTNLM
OT  - Antioxidant enzymes
OT  - Gene expression
OT  - Gene silencing
OT  - HIF-1alpha
OT  - Viral load
OT  - WSSV
EDAT- 2018/07/25 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/07/25 06:00
PHST- 2018/05/24 00:00 [received]
PHST- 2018/07/17 00:00 [revised]
PHST- 2018/07/18 00:00 [accepted]
PHST- 2018/07/25 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/07/25 06:00 [entrez]
AID - S1532-0456(18)30094-2 [pii]
AID - 10.1016/j.cbpc.2018.07.004 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2018 Nov;213:19-26. doi: 
      10.1016/j.cbpc.2018.07.004. Epub 2018 Jul 21.