PMID- 30041241
OWN - NLM
STAT- MEDLINE
DCOM- 20190919
LR  - 20211204
IS  - 1422-6421 (Electronic)
IS  - 1422-6405 (Linking)
VI  - 206
IP  - 1-2
DP  - 2018
TI  - Sulforaphane, a Chemopreventive Compound, Inhibits Cyclooxygenase-2 and 
      Microsomal Prostaglandin E Synthase-1 Expression in Human HT-29 Colon Cancer 
      Cells.
PG  - 46-53
LID - 10.1159/000490394 [doi]
AB  - BACKGROUND: A high expression of prostaglandin E2 (PGE2) is found in colorectal 
      cancer. Therefore, blocking of PGE2 generation has been identified as a promising 
      approach for anticancer therapy. Sulforaphane (SFN), an isothiocyanate derived 
      from glucosinolate, is used as the antioxidant and anticancer agents. METHODS: 
      HT-29 cells were treated with various concentrations of SFN and compared to 
      untreated cells for the expression of microsomal prostaglandin E synthase-1 
      (mPGES-1), cyclooxygenase 2 (COX-2), hypoxia-inducible factor-1 (HIF-1), C-X-C 
      chemokine receptor type 4 (CXCR4), vascular endothelial growth factor (VEGF), and 
      matrix metalloproteinase (MMP)-2 and MMP-9 at the mRNA level. The PGE2 level was 
      measured by ELISA assay. Apoptosis was evaluated by the proportion of sub-G1 
      cells. The activity of caspase-3 was determined using an enzymatic assay. HT-29 
      cell migration was assessed using a scratch test. RESULTS: SFN preconditioning 
      decreased the expression of COX-2, mPGES-1, HIF-1, VEGF, CXCR4, MMP-2, and MMP-9. 
      An apoptotic effect of SFN was preceded by the activation of caspase-3 as well as 
      accumulation of cells in the sub-G1 phase of the cell cycle. SFN decreased PGE2 
      generation and inhibited the in vitro motility/wound-healing activity of HT-29 
      cells. CONCLUSIONS: SFN anticancer effects are associated with antiproliferative, 
      antiangiogenic, and antimetastatic activities arising from the downregulation of 
      the COX-2/ mPGES-1 axis.
CI  - (c) 2018 S. Karger AG, Basel.
FAU - Tafakh, Maryam Sadat
AU  - Tafakh MS
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Saidijam, Massoud
AU  - Saidijam M
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Ranjbarnejad, Tayebeh
AU  - Ranjbarnejad T
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Malih, Sara
AU  - Malih S
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Mirzamohammadi, Solmaz
AU  - Mirzamohammadi S
AD  - Department of Pharmacology, Shahroud University of Medical Sciences, Sharoud, 
      Iran.
FAU - Najafi, Rezvan
AU  - Najafi R
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran, najafi2535@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180724
PL  - Switzerland
TA  - Cells Tissues Organs
JT  - Cells, tissues, organs
JID - 100883360
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (Cyclooxygenase 2 Inhibitors)
RN  - 0 (Isothiocyanates)
RN  - 0 (Sulfoxides)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 5.3.99.3 (PTGES protein, human)
RN  - EC 5.3.99.3 (Prostaglandin-E Synthases)
RN  - GA49J4310U (sulforaphane)
SB  - IM
MH  - Anticarcinogenic Agents/*pharmacology
MH  - Cell Survival/drug effects
MH  - Colonic Neoplasms/*drug therapy/genetics/metabolism/prevention & control
MH  - Cyclooxygenase 2/metabolism
MH  - Cyclooxygenase 2 Inhibitors/*pharmacology
MH  - Down-Regulation/drug effects
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - HT29 Cells
MH  - Humans
MH  - Isothiocyanates/*pharmacology
MH  - Prostaglandin-E Synthases/*antagonists & inhibitors/genetics
MH  - Sulfoxides
OTO - NOTNLM
OT  - Colorectal cancer
OT  - Cyclooxygenase-2
OT  - Microsomal prostaglandin E synthase-1
OT  - Prostaglandin E2
OT  - Sulforaphane
EDAT- 2018/07/25 06:00
MHDA- 2019/09/20 06:00
CRDT- 2018/07/25 06:00
PHST- 2017/09/24 00:00 [received]
PHST- 2018/05/25 00:00 [accepted]
PHST- 2018/07/25 06:00 [pubmed]
PHST- 2019/09/20 06:00 [medline]
PHST- 2018/07/25 06:00 [entrez]
AID - 000490394 [pii]
AID - 10.1159/000490394 [doi]
PST - ppublish
SO  - Cells Tissues Organs. 2018;206(1-2):46-53. doi: 10.1159/000490394. Epub 2018 Jul 
      24.