PMID- 30042495
OWN - NLM
STAT- MEDLINE
DCOM- 20191023
LR  - 20191023
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Jul 24
TI  - The Impact of Ageing on 11C-Hydroxyephedrine Uptake in the Rat Heart.
PG  - 11120
LID - 10.1038/s41598-018-29509-0 [doi]
LID - 11120
AB  - We aimed to explore the impact of ageing on 11C-hydroxyephedrine (11C-HED) uptake 
      in the healthy rat heart in a longitudinal setting. To investigate a potential 
      cold mass effect, the influence of specific activity on cardiac 11C-HED uptake 
      was evaluated: 11C-HED was synthesized by N-methylation of (-)-metaraminol as the 
      free base (radiochemical purity >95%) and a wide range of specific activities 
      (0.2-141.9 GBq/mumol) were prepared. (11)C-HED (48.7 +/- 9.7MBq, ranged 
      0.2-60.4 mug/kg cold mass) was injected in healthy Wistar Rats. Dynamic 23-frame 
      PET images were obtained over 30 min. Time activity curves were generated for the 
      blood input function and myocardial tissue. Cardiac 11C-HED retention index 
      (%/min) was calculated as myocardial tissue activity at 20-30 min divided by the 
      integral of the blood activity curves. Additionally, the impact of ageing on 
      myocardial 11C-HED uptake was investigated longitudinally by PET studies at 
      different ages of healthy Wistar Rats. A dose-dependent reduction of cardiac 
      11C-HED uptake was observed: The estimated retention index as a marker of 
      norepinephrine function decreased at a lower specific activity (higher amount of 
      cold mass). This observed high affinity of 11C-HED to the neural norepinephrine 
      transporter triggered a subsequent study: In a longitudinal setting, the 11C-HED 
      retention index decreased with increasing age. An age-related decline of cardiac 
      sympathetic innervation could be demonstrated. The herein observed cold mass 
      effect might increase in succeeding scans and therefore, 11C-HED microPET studies 
      should be planned with extreme caution if one single radiosynthesis is scheduled 
      for multiple animals.
FAU - Werner, Rudolf A
AU  - Werner RA
AD  - Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany.
AD  - The Russell H. Morgan Department of Radiology and Radiological Science, Division 
      of Nuclear Medicine and Molecular Imaging, Johns Hopkins University School of 
      Medicine, Baltimore, MD, United States.
AD  - Comprehensive Heart Failure Center (CHFC), University of Wurzburg, Wurzburg, 
      Germany.
FAU - Chen, Xinyu
AU  - Chen X
AD  - Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany.
AD  - Comprehensive Heart Failure Center (CHFC), University of Wurzburg, Wurzburg, 
      Germany.
FAU - Maya, Yoshifumi
AU  - Maya Y
AD  - Research Centre, Nihon Medi-Physics Co., Ltd., Chiba, Japan.
FAU - Eissler, Christoph
AU  - Eissler C
AD  - Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany.
FAU - Hirano, Mitsuru
AU  - Hirano M
AD  - Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany.
AD  - Comprehensive Heart Failure Center (CHFC), University of Wurzburg, Wurzburg, 
      Germany.
FAU - Nose, Naoko
AU  - Nose N
AD  - Department of Biomedical Imaging, National Cerebral and Cardiovascular Center, 
      Suita, Japan.
FAU - Wakabayashi, Hiroshi
AU  - Wakabayashi H
AD  - Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany.
AD  - Comprehensive Heart Failure Center (CHFC), University of Wurzburg, Wurzburg, 
      Germany.
FAU - Lapa, Constantin
AU  - Lapa C
AD  - Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany.
FAU - Javadi, Mehrbod S
AU  - Javadi MS
AD  - The Russell H. Morgan Department of Radiology and Radiological Science, Division 
      of Nuclear Medicine and Molecular Imaging, Johns Hopkins University School of 
      Medicine, Baltimore, MD, United States.
FAU - Higuchi, Takahiro
AU  - Higuchi T
AD  - Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany. 
      thiguchi@me.com.
AD  - Comprehensive Heart Failure Center (CHFC), University of Wurzburg, Wurzburg, 
      Germany. thiguchi@me.com.
AD  - Department of Biomedical Imaging, National Cerebral and Cardiovascular Center, 
      Suita, Japan. thiguchi@me.com.
LA  - eng
GR  - 1789/3-3/Deutsche Forschungsgemeinschaft (German Research 
      Foundation)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180724
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Carbon Radioisotopes)
RN  - 3002-40-2 (3-hydroxyephedrine)
RN  - 818U2PZ2EH (Metaraminol)
RN  - GN83C131XS (Ephedrine)
SB  - IM
MH  - Aging/*metabolism/pathology
MH  - Animals
MH  - Carbon Radioisotopes/administration & dosage
MH  - Ephedrine/administration & dosage/*analogs & derivatives
MH  - Heart/*diagnostic imaging/innervation/physiology
MH  - Humans
MH  - Metaraminol/chemistry
MH  - Myocardium/*metabolism
MH  - Positron-Emission Tomography
MH  - Radionuclide Imaging/methods
MH  - Rats
MH  - Rats, Wistar
MH  - Sympathetic Nervous System
MH  - Tissue Distribution
PMC - PMC6057985
COIS- The authors declare no competing interests.
EDAT- 2018/07/26 06:00
MHDA- 2019/10/24 06:00
PMCR- 2018/07/24
CRDT- 2018/07/26 06:00
PHST- 2018/05/16 00:00 [received]
PHST- 2018/07/11 00:00 [accepted]
PHST- 2018/07/26 06:00 [entrez]
PHST- 2018/07/26 06:00 [pubmed]
PHST- 2019/10/24 06:00 [medline]
PHST- 2018/07/24 00:00 [pmc-release]
AID - 10.1038/s41598-018-29509-0 [pii]
AID - 29509 [pii]
AID - 10.1038/s41598-018-29509-0 [doi]
PST - epublish
SO  - Sci Rep. 2018 Jul 24;8(1):11120. doi: 10.1038/s41598-018-29509-0.