PMID- 30045250
OWN - NLM
STAT- MEDLINE
DCOM- 20180731
LR  - 20210109
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 97
IP  - 30
DP  - 2018 Jul
TI  - Associations of human leukocyte antigen and interleukin-18 gene polymorphisms 
      with viral load in patients with hepatitis B infection.
PG  - e11249
LID - 10.1097/MD.0000000000011249 [doi]
LID - e11249
AB  - This study aimed to assess the associations of human leukocyte antigen (HLA)-DR 
      and interleukin (IL)-18 gene polymorphisms with hepatitis B virus (HBV).Clinical 
      data were retrospectively reviewed between December 2006 and December 2015 at 
      Xiangyang Central Hospital. HBV patients were assigned to the high and low viral 
      load groups, respectively, according to HBV copies. HLA-DRB1*03 polymorphisms and 
      IL-18 polymorphisms were detected by sequence-specific primer-polymerase chain 
      reaction (PCR-SSP) and PCR-ligase detection reaction (PCR-LDR), respectively. T 
      cell subgroups were identified by flow cytometry, and IL-18, IL-12, interferon-gamma 
      (IFN-gamma), IL-4, and IL-10 expression levels were assessed by ELISA. A total of 630 
      subjects were included in the analysis.Compared with healthy controls, the 
      chronic HBV group showed significantly lower IL-18 (P < .001), IL-12 (P < .001), 
      and IFN-gamma (P < .001) expression levels, and markedly increased IL-4 (P < .001) 
      and IL-10 (P < .001) amounts. Th2 cytokine expression was high in HLA-DRB1*03 
      positive (+) HBV patients, with low Th1 cytokine levels. The ratios of CD4+/CD8+ 
      and Th1/Th2 cells decreased with increasing HBV DNA levels. The chronic HBV group 
      showed a relatively high frequency of -137G in the IL-18 gene, while IL-18 
      expression was low in homozygous GG genotype individuals.Polymorphisms in the 
      HLA-DRB1*03 and IL-18 genes are associated with viral load in HBV. HLA-DRB1 and 
      IL-18 gene polymorphisms are involved in the regulation of the Th1/Th2 balance 
      and expression of relevant cytokines that influence immune responses in HBV.
FAU - Jiang, Hua
AU  - Jiang H
AD  - Department of Clinical Laboratory Department of Intensive Care Unit Department of 
      Gastroenterology, Hospital Affiliated to Hubei University of Arts and Science, 
      Xiangyang, Hubei, People's Republic of China.
FAU - Cao, Fengsheng
AU  - Cao F
FAU - Cao, Hong
AU  - Cao H
FAU - Rao, Qun
AU  - Rao Q
FAU - Yang, Ying
AU  - Yang Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (HLA-DR Antigens)
RN  - 0 (IL18 protein, human)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Adult
MH  - China
MH  - Female
MH  - Gene Frequency
MH  - HLA-DR Antigens/*genetics
MH  - Hepatitis B virus/isolation & purification
MH  - *Hepatitis B, Chronic/diagnosis/genetics
MH  - Humans
MH  - Interleukin-18/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
MH  - Viral Load/*genetics
PMC - PMC6078658
COIS- The authors declare no conflicts of interest.
EDAT- 2018/07/26 06:00
MHDA- 2018/08/01 06:00
PMCR- 2018/07/27
CRDT- 2018/07/26 06:00
PHST- 2018/07/26 06:00 [entrez]
PHST- 2018/07/26 06:00 [pubmed]
PHST- 2018/08/01 06:00 [medline]
PHST- 2018/07/27 00:00 [pmc-release]
AID - 00005792-201807270-00003 [pii]
AID - MD-D-17-06310 [pii]
AID - 10.1097/MD.0000000000011249 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2018 Jul;97(30):e11249. doi: 10.1097/MD.0000000000011249.