PMID- 30045257
OWN - NLM
STAT- MEDLINE
DCOM- 20180731
LR  - 20220419
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 97
IP  - 30
DP  - 2018 Jul
TI  - Fluorescence in situ hybridization microscopic detection of Bacilli Calmette 
      Guerin mycobacteria in aortic lesions: A case report.
PG  - e11321
LID - 10.1097/MD.0000000000011321 [doi]
LID - e11321
AB  - RATIONALE: To improve the diagnosis of life-threatening Bacilli Calmette Guerin 
      (BCG) arterial aneurysm in patients treated by intravesical instillation of BCG 
      vaccine as adjunctive therapy for non-muscular bladder carcinoma, is a 
      life-threatening condition. Its diagnosis remains cumbersome. PATIENT CONCERNS: 
      One patient with a history of intravesical BCG installation presented with aortic 
      aneurysm with routine microscopic examination after Ziehl-Neelsen staining 
      remaining negative. DIAGNOSES: We used fluorescence in situ hybridization (FISH) 
      to target the Mycobacterium tuberculosis complex rpob gene in a fresh aortic 
      specimen. FISH yielded fluorescent mycobacteria in aortic lesions; mycobacteria 
      were further confirmed as Mycobacterium bovis BCG mycobacteria by polymerase 
      chain reaction (PCR) sequencing. INTERVENTIONS: The patient benefited from an 
      antituberculous treatment combining rifampicin, isoniazid, and ethambunol. 
      OUTCOME: A 9-month follow-up indicated a favorable outcome. LESSONS: This case 
      report teaches that FISH targeting the M tuberculosis complex rpoB gene should be 
      incorporated in the laboratory investigation of aortic aneurysm in patients with 
      a history of bladder carcinoma.
FAU - Darriet, Florent
AU  - Darriet F
AD  - Microbiology Laboratory Infectious Disease Department, Assistance Publique 
      -Hopitaux de Marseille Aix-Marseille University, Institut de Recherche pour le 
      Developpement, Microbes, Evolution, Phylogenie, Infections, Institut Hospitalier 
      Universitaire Mediterranee-Infection, Marseille, France.
FAU - Bernioles, Paola
AU  - Bernioles P
FAU - Loukil, Ahmed
AU  - Loukil A
FAU - Saidani, Nadia
AU  - Saidani N
FAU - Eldin, Carole
AU  - Eldin C
FAU - Drancourt, Michel
AU  - Drancourt M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (BCG Vaccine)
SB  - IM
MH  - Adjuvants, Immunologic/administration & dosage/adverse effects
MH  - Administration, Intravesical
MH  - Aged
MH  - *Aortic Aneurysm, Abdominal/diagnosis/etiology/microbiology/physiopathology
MH  - *BCG Vaccine/administration & dosage/adverse effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Microscopy, Fluorescence/*methods
MH  - Mycobacterium bovis/*isolation & purification
MH  - Reproducibility of Results
MH  - Urinary Bladder Neoplasms/*drug therapy
PMC - PMC6078740
COIS- The authors declare that there are no competing interests regarding the 
      publication of this paper.
EDAT- 2018/07/26 06:00
MHDA- 2018/08/01 06:00
PMCR- 2018/07/27
CRDT- 2018/07/26 06:00
PHST- 2018/07/26 06:00 [entrez]
PHST- 2018/07/26 06:00 [pubmed]
PHST- 2018/08/01 06:00 [medline]
PHST- 2018/07/27 00:00 [pmc-release]
AID - 00005792-201807270-00010 [pii]
AID - MD-D-18-01011 [pii]
AID - 10.1097/MD.0000000000011321 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2018 Jul;97(30):e11321. doi: 10.1097/MD.0000000000011321.