PMID- 30046953
OWN - NLM
STAT- MEDLINE
DCOM- 20181127
LR  - 20200324
IS  - 1476-5535 (Electronic)
IS  - 1367-5435 (Print)
IS  - 1367-5435 (Linking)
VI  - 45
IP  - 10
DP  - 2018 Oct
TI  - Surface-displayed porcine reproductive and respiratory syndrome virus from cell 
      culture onto gram-positive enhancer matrix particles.
PG  - 889-898
LID - 10.1007/s10295-018-2061-1 [doi]
AB  - Vaccine immunization is now one of the most effective ways to control porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of 
      the main factors affecting vaccine safety and efficacy. Here we present a novel 
      innovative PRRSV purification approach based on surface display technology. 
      First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial 
      factor in the purification process, was successfully produced in Escherichia coli 
      yielding 80 mg/L of broth culture. Then PRRSV purification was performed by 
      incubation of PA-GRFT with PRRSV and gram-positive enhancer matrix (GEM) 
      particles, followed by centrifugation to collect virions loaded onto GEM 
      particles. Our results showed that most of the bulk impurities had been removed, 
      and PA-GRFT could capture PRRSV onto GEM particles. Our lactic acid 
      bacteria-based purification method, which is promising as ease of operation, low 
      cost and easy to scale-up, may represent a candidate method for the large-scale 
      purification of this virus for vaccine production.
FAU - Li, Lan
AU  - Li L
AD  - National Research, Center of Engineering and Technology for Veterinary 
      Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, 
      China.
FAU - Qiao, Xuwen
AU  - Qiao X
AD  - National Research, Center of Engineering and Technology for Veterinary 
      Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, 
      China.
FAU - Chen, Jin
AU  - Chen J
AD  - National Research, Center of Engineering and Technology for Veterinary 
      Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, 
      China.
FAU - Zhang, Yuanpeng
AU  - Zhang Y
AD  - National Research, Center of Engineering and Technology for Veterinary 
      Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, 
      China.
FAU - Zheng, Qisheng
AU  - Zheng Q
AD  - National Research, Center of Engineering and Technology for Veterinary 
      Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, 
      China. njcvc1302@163.com.
FAU - Hou, Jibo
AU  - Hou J
AD  - National Research, Center of Engineering and Technology for Veterinary 
      Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, 
      China. houjiboccvv@163.com.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China. 
      houjiboccvv@163.com.
LA  - eng
GR  - 201303046/the Special Fund for Agro-scientific Research in the Public Interest/
GR  - cx (14)2089/the Independent Innovation of Agricultural Sciences Program of 
      Jiangsu Province/
PT  - Journal Article
DEP - 20180725
PL  - Germany
TA  - J Ind Microbiol Biotechnol
JT  - Journal of industrial microbiology & biotechnology
JID - 9705544
RN  - 0 (Lectins)
RN  - 0 (Vaccines)
RN  - 0 (Viral Vaccines)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA/analysis
MH  - Dose-Response Relationship, Drug
MH  - Escherichia coli/metabolism
MH  - Genetic Engineering
MH  - Immunization
MH  - Lactobacillales/chemistry
MH  - Lectins
MH  - Microscopy, Fluorescence
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Surface Properties
MH  - Swine
MH  - Temperature
MH  - Vaccines/chemistry
MH  - Viral Vaccines/*immunology
MH  - *Virus Cultivation
PMC - PMC7088258
OTO - NOTNLM
OT  - GEM particles
OT  - Griffithsin
OT  - Lactic acid bacteria
OT  - PRRSV
OT  - Purification
COIS- There is no conflict of interests.
EDAT- 2018/07/27 06:00
MHDA- 2018/11/28 06:00
PMCR- 2020/03/23
CRDT- 2018/07/27 06:00
PHST- 2018/01/25 00:00 [received]
PHST- 2018/07/13 00:00 [accepted]
PHST- 2018/07/27 06:00 [pubmed]
PHST- 2018/11/28 06:00 [medline]
PHST- 2018/07/27 06:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 10.1007/s10295-018-2061-1 [pii]
AID - 2061 [pii]
AID - 10.1007/s10295-018-2061-1 [doi]
PST - ppublish
SO  - J Ind Microbiol Biotechnol. 2018 Oct;45(10):889-898. doi: 
      10.1007/s10295-018-2061-1. Epub 2018 Jul 25.