PMID- 30048989
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 177
IP  - 3
DP  - 2018
TI  - MicroRNA-126 Deficiency Affects the Development of Thymus CD4+ Single-Positive 
      Cells through Elevating IRS-1.
PG  - 207-218
LID - 10.1159/000490710 [doi]
AB  - BACKGROUND: MicroRNA-126 (miR-126), a distinct miRNA family member, has been 
      reported to be involved in the development and function of some types of immune 
      cells. However, the potential role of miR-126 in the development of CD4+ T cells 
      remains to be elucidated. OBJECTIVES: To investigate the potential role of 
      miR-126 in the development of CD4+ T cells in the thymus and explore its 
      significance. METHODS: The relative expression level of miR-126 in thymus CD4+ 
      single-positive (SP) cells was detected by Real-Time PCR assay. The possible 
      change in thymus tissue was assessed by histopathology. The total cell number of 
      thymocytes and the expression of activation-associated molecules including CD62L, 
      CD69, and CD44, as well as proliferation-associated nuclear antigen Ki-67, in 
      CD4+ SP cells were assessed by flow cytometric analysis. The expression of IRS-1 
      and related signaling pathways including Akt and Erk were determined by flow 
      cytometric analysis. RESULTS: Compared with that in wild-type (WT) mice, the 
      total cell number of thymocytes in miR-126 knockdown (KD) mice increased 
      significantly. Moreover, the proportion and absolute cell number of thymic CD4+ 
      SP cells decreased significantly in miR-126 KD mice. Further analysis showed that 
      the frequencies of activation-associated molecules including CD62L, CD69, and 
      CD44, as well as proliferation-associated nuclear antigen Ki-67 in CD4+ SP cells 
      also changed significantly, respectively. Mechanism aspect, the expression level 
      of IRS-1, a putative target of miR-126, increased significantly in CD4+ SP cells 
      in miR-126 KD mice. Moreover, the expression levels of the signaling molecules 
      phosphorylated (p)-Akt and p-Erk also changed significantly. CONCLUSIONS: Our 
      work is the first to reveal a previously unknown role of miR-126 in the 
      development of CD4+ SP cells in the thymus, which might ultimately benefit 
      studies on development of thymocytes.
CI  - (c) 2018 S. Karger AG, Basel.
FAU - Hu, Lin
AU  - Hu L
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Xu, Hualin
AU  - Xu H
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Lu, Jia
AU  - Lu J
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Zhou, Ya
AU  - Zhou Y
AD  - Department of Medical Physics, Zunyi Medical University, Zunyi, China.
FAU - Chu, Fengyun
AU  - Chu F
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Zheng, Wen
AU  - Zheng W
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Lei, Liangyu
AU  - Lei L
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Zhao, Juanjuan
AU  - Zhao J
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Wang, Hairong
AU  - Wang H
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Guo, Mengmeng
AU  - Guo M
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Chen, Chao
AU  - Chen C
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
FAU - Xu, Lin
AU  - Xu L
AD  - Special Key Laboratory of Gene Detection & Therapy of Guizhou Provincial 
      Education Department, Zunyi, China.
AD  - Department of Immunology & Talent Base of Biological Therapy of Guizhou Province, 
      Zunyi Medical University, Zunyi, China.
LA  - eng
PT  - Journal Article
DEP - 20180726
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD69 antigen)
RN  - 0 (Cd44 protein, mouse)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, mouse)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Lectins, C-Type)
RN  - 0 (MIRN126 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mki67 protein, mouse)
RN  - 126880-86-2 (L-Selectin)
SB  - IM
MH  - Animals
MH  - Antigens, CD/biosynthesis
MH  - Antigens, Differentiation, T-Lymphocyte/biosynthesis
MH  - CD4-Positive T-Lymphocytes/*cytology/*immunology
MH  - Cell Differentiation/*genetics/immunology
MH  - Flow Cytometry
MH  - Hyaluronan Receptors/biosynthesis
MH  - Insulin Receptor Substrate Proteins/metabolism
MH  - Ki-67 Antigen/biosynthesis
MH  - L-Selectin/biosynthesis
MH  - Lectins, C-Type/biosynthesis
MH  - Lymphocyte Activation/*genetics/immunology
MH  - Mice
MH  - Mice, Knockout
MH  - MicroRNAs/*genetics
MH  - Thymocytes/cytology/immunology
MH  - Thymus Gland/cytology
OTO - NOTNLM
OT  - IRS-1
OT  - Knockdown
OT  - MicroRNA-126
OT  - SP cells
OT  - Thymus
EDAT- 2018/07/27 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/07/27 06:00
PHST- 2018/04/03 00:00 [received]
PHST- 2018/06/07 00:00 [accepted]
PHST- 2018/07/27 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/07/27 06:00 [entrez]
AID - 000490710 [pii]
AID - 10.1159/000490710 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2018;177(3):207-218. doi: 10.1159/000490710. Epub 2018 
      Jul 26.