PMID- 30049418
OWN - NLM
STAT- MEDLINE
DCOM- 20190625
LR  - 20191210
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 223
IP  - 11
DP  - 2018 Nov
TI  - PEP-1-PEA15 suppresses inflammatory responses by regulation of MAPK in 
      macrophages and animal models.
PG  - 709-717
LID - S0171-2985(18)30062-7 [pii]
LID - 10.1016/j.imbio.2018.07.019 [doi]
AB  - Phosphoprotein enriched in astrocytes 15 (PEA15) plays a multi-functional role in 
      neuronal cell survival, however the effects of PEA15 against inflammation have 
      not been investigated yet. To examine the effects of PEP-1-PEA15 protein against 
      lipopolysaccharide (LPS)-induced inflammatory responses in Raw 264.7 cells and in 
      a 12-O-tetradecanoylphobol 13-acetate (TPA)-induced mouse model, we constructed 
      and purified PEP-1-PEA15 protein, which can transduce into cells or tissues. 
      PEP-1-PEA15 inhibited LPS-induced damage in cells including that caused by 
      reactive oxygen species (ROS) production and DNA fragmentation. PEP-1-PEA15 also 
      significantly suppressed activation of mitogen activated protein kinases (MAPKs), 
      pro-inflammatory mediator proteins and various cytokines. In a TPA-induced mouse 
      ear edema model, PEP-1-PEA15 significantly reduced ear weight and thickness as 
      well as MAPK activation as well as the expression levels of COX-2, iNOS, IL-6, 
      IL-1beta, and TNF-alpha. These results demonstrated that PEP-1-PEA15 showed 
      anti-inflammatory effect in cells and animal model suggesting that this fusion 
      protein protects cells or skin tissues from inflammatory response.
CI  - Copyright (c) 2018 Elsevier GmbH. All rights reserved.
FAU - Yong, Ji In
AU  - Yong JI
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Kim, Dae Won
AU  - Kim DW
AD  - Department of Biochemistry and Molecular Biology, Research Institute of Oral 
      Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung 
      25457, Republic of Korea.
FAU - Shin, Min Jea
AU  - Shin MJ
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Jo, Hyo Sang
AU  - Jo HS
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Park, Jung Hwan
AU  - Park JH
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Cho, Su Bin
AU  - Cho SB
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Lee, Chi Hern
AU  - Lee CH
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Yeo, Hyeon Ji
AU  - Yeo HJ
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Yeo, Eun Ji
AU  - Yeo EJ
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Choi, Yeon Joo
AU  - Choi YJ
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Kim, Ji An
AU  - Kim JA
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Hwang, Jung Soon
AU  - Hwang JS
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Kim, Duk-Soo
AU  - Kim DS
AD  - Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan-Si 
      31538, Republic of Korea.
FAU - Kim, Hyun Ah
AU  - Kim HA
AD  - Division of Rheumatology, Department of Internal Medicine, Hallym University 
      Sacred Heart Hospital, Kyunggi 14068, Republic of Korea.
FAU - Cho, Yong-Jun
AU  - Cho YJ
AD  - Department of Neurosurgery, Hallym University Medical Center, Chuncheon 24253, 
      Republic of Korea.
FAU - Lee, Keun Wook
AU  - Lee KW
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Han, Kyu Hyung
AU  - Han KH
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Park, Jinseu
AU  - Park J
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
FAU - Eum, Won Sik
AU  - Eum WS
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea. Electronic 
      address: wseum@hallym.ac.kr.
FAU - Choi, Soo Young
AU  - Choi SY
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea. Electronic 
      address: sychoi@hallym.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180718
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (PEA15 protein, human)
RN  - 0 (Pep-1 peptide)
RN  - 0 (Peptides)
RN  - 0 (Phosphoproteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 5UX2SD1KE2 (Cysteamine)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Apoptosis Regulatory Proteins
MH  - Cysteamine/*analogs & derivatives/metabolism
MH  - Cytokines/metabolism
MH  - DNA Fragmentation
MH  - Disease Models, Animal
MH  - Edema/*immunology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Humans
MH  - Inflammation/*immunology
MH  - Inflammation Mediators/metabolism
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Lipopolysaccharides/immunology
MH  - Macrophages/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Peptides/*metabolism
MH  - Phosphoproteins/*metabolism
MH  - RAW 264.7 Cells
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction
MH  - Tetradecanoylphorbol Acetate/immunology
OTO - NOTNLM
OT  - Cytokines
OT  - Inflammation
OT  - MAPK
OT  - PEP-1-PEA15
OT  - Protein therapy
EDAT- 2018/07/28 06:00
MHDA- 2019/06/27 06:00
CRDT- 2018/07/28 06:00
PHST- 2018/04/29 00:00 [received]
PHST- 2018/07/10 00:00 [revised]
PHST- 2018/07/17 00:00 [accepted]
PHST- 2018/07/28 06:00 [pubmed]
PHST- 2019/06/27 06:00 [medline]
PHST- 2018/07/28 06:00 [entrez]
AID - S0171-2985(18)30062-7 [pii]
AID - 10.1016/j.imbio.2018.07.019 [doi]
PST - ppublish
SO  - Immunobiology. 2018 Nov;223(11):709-717. doi: 10.1016/j.imbio.2018.07.019. Epub 
      2018 Jul 18.