PMID- 30050131
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20190726
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 9
IP  - 1
DP  - 2018 Jul 26
TI  - Concerted ESCRT and clathrin recruitment waves define the timing and morphology 
      of intraluminal vesicle formation.
PG  - 2932
LID - 10.1038/s41467-018-05345-8 [doi]
LID - 2932
AB  - The endosomal sorting complex required for transport (ESCRT) machinery mediates 
      cargo sorting, membrane deformation and membrane scission on the surface of 
      endosomes, generating intraluminal vesicles (ILVs) to degrade signaling 
      receptors. By live-cell imaging of individual endosomes in human cells, we find 
      that ESCRT proteins are recruited in a repetitive pattern: ESCRT-0 and -I show a 
      gradual and linear recruitment and dissociation, whereas ESCRT-III and its 
      regulatory ATPase VPS4 display fast and transient dynamics. Electron microscopy 
      shows that ILVs are formed consecutively, starting immediately after endocytic 
      uptake of cargo proteins and correlating with the repeated ESCRT recruitment 
      waves, unraveling the timing of ILV formation. Clathrin, recruited by ESCRT-0, is 
      required for timely ESCRT-0 dissociation, efficient ILV formation, correct ILV 
      size and cargo degradation. Thus, cargo sorting and ILV formation occur by 
      concerted, coordinated and repetitive recruitment waves of individual ESCRT 
      subcomplexes and are controlled by clathrin.
FAU - Wenzel, Eva Maria
AU  - Wenzel EM
AUID- ORCID: 0000-0002-5561-3344
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway. eva.wenzel@medisin.uio.no.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway. eva.wenzel@medisin.uio.no.
FAU - Schultz, Sebastian Wolfgang
AU  - Schultz SW
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway.
FAU - Schink, Kay Oliver
AU  - Schink KO
AUID- ORCID: 0000-0002-5903-4059
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway.
FAU - Pedersen, Nina Marie
AU  - Pedersen NM
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway.
FAU - Nahse, Viola
AU  - Nahse V
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway.
FAU - Carlson, Andreas
AU  - Carlson A
AUID- ORCID: 0000-0002-3068-9983
AD  - Department of Mathematics, University of Oslo, N-0316, Oslo, Norway.
FAU - Brech, Andreas
AU  - Brech A
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway.
FAU - Stenmark, Harald
AU  - Stenmark H
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway.
FAU - Raiborg, Camilla
AU  - Raiborg C
AUID- ORCID: 0000-0002-5406-5403
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Oslo 
      University Hospital, Montebello, N-0379, Oslo, Norway. 
      camilla.raiborg@rr-research.no.
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, N-0316, Oslo, Norway. 
      camilla.raiborg@rr-research.no.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180726
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Clathrin)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
SB  - IM
MH  - Biological Transport
MH  - Clathrin/*metabolism
MH  - Endosomal Sorting Complexes Required for Transport/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Multivesicular Bodies
MH  - Protein Transport
PMC - PMC6062606
COIS- The authors declare no competing interests.
EDAT- 2018/07/28 06:00
MHDA- 2018/12/18 06:00
PMCR- 2018/07/26
CRDT- 2018/07/28 06:00
PHST- 2017/12/09 00:00 [received]
PHST- 2018/06/29 00:00 [accepted]
PHST- 2018/07/28 06:00 [entrez]
PHST- 2018/07/28 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
PHST- 2018/07/26 00:00 [pmc-release]
AID - 10.1038/s41467-018-05345-8 [pii]
AID - 5345 [pii]
AID - 10.1038/s41467-018-05345-8 [doi]
PST - epublish
SO  - Nat Commun. 2018 Jul 26;9(1):2932. doi: 10.1038/s41467-018-05345-8.